Phloretin-induced Apoptosis in B16 Melanoma 4A5 Cells and HL60 Human Leukemia Cells

Kobori, Masuko; Iwashita, Keiko; Shinmoto, Hiroshi; Tsushida, Tojiro

doi:10.1271/bbb.63.719

Cited by 71 publications

(47 citation statements)

References 30 publications

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“…Data are shown as the mean ± SD (n=3). have reported the anti-proliferative effects of PT on HL-60, HT29 and HepG2 cells, the effective dosages of PT were all >100 µM (18,20,22). Concordantly, our results showed that, in both SW620 and HCT116 cells, PT could not efficiently inhibit cell growth unless the dose was higher than 100 µM.…”

Section: Discussionsupporting

confidence: 80%

“…Phloretin (PT) is one of the most abundant phenolic phytochemicals in apples and apple products. Numerous studies have reported on the antitumor activities of PT, including its ability to suppress cell growth and induce apoptosis in human hepatoma cells, HL-60 human leukemia cells, B16 mouse 4A5 melanoma cells and HT29 human colon cancer cells (18)(19)(20)(21). Both in vitro and in vivo studies have revealed that PT could potentiate the antitumor effects of paclitaxel via the induction of cell apoptosis (22).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic inhibition of colon cancer cell growth by a combination of atorvastatin and phloretin

Zhou

Zheng

et al. 2017

Oncol Lett

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Abstract. Atorvastatin (ATST), a drug commonly used to reduce the levels of cholesterol and low-density lipoproteins, is a prospective agent for the prevention of colorectal cancer in patients with hyperlipidemia. ATST in combination with functional components is a promising strategy for cancer chemoprevention. In the present study, the growth inhibitory effect of ATST combined with phloretin (PT) on SW620 and HCT116 colon cancer cells was investigated. The results of MTT assays indicated that the combination of PT and ATST markedly reduced cell survival in both cell lines compared with PT or ATST treatment administered individually. The interaction indexes between PT and ATST, which were used to analyze their interaction pattern, were computed by the median-effect equation. The interaction indexes of each PT and ATST concentration pair were <1.0, which indicated a strong synergistic effect between the two compounds. The data obtained by flow cytometry and western blot analysis of cleaved-poly (ADP-ribose) polymerase indicated a synergistic effect resulted in apoptosis and cell cycle arrest at the G 2 /M checkpoint. Furthermore, combined treatment with PT and ATST markedly downregulated the expression of cyclin B and upregulated the expression of phospho-cdc2 and Myt1, which suggested that the activation of cdc2 was downregulated. This combined treatment strategy enhanced the anti-cancer activity of ATST at a relatively low dosage and suggested a possible method of preventing colorectal cancer in patients with hyperlipidemia.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Synergistic inhibition of colon cancer cell growth by a combination of atorvastatin and phloretin

Zhou

Zheng

et al. 2017

Oncol Lett

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“…Although the role of apoptosis in pathological situation for cancer suppression has been already evaluated [9][10][11] . This study demonstrates that antioxidants have the potential scavenging effect on intracellular oxidative damage directly induced by H 2 O 2 which, in turn, lead to an improvement in the percentage cell viability.…”

Section: Discussionmentioning

confidence: 99%

“…Apoptosis, in association with oxidative stress, is an active cell death mechanism, which occurs during several pathological situations for cancer suppression [9][10][11] . It can be induced by two different pathways, the deathreceptor pathway and mitochondrial pathway (mediated by caspase-8 and caspase-9, respectively).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anti–apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide

Rai

Mangal

Sahu

et al. 2011

Asian Pacific Journal of Tropical Biomedicine

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“…Therefore, the inhibition of cancer cell growth and the induction of apoptotic cancer cell death are important mechanisms of cancer prevention. Apoptosis is one of the major mechanisms of cancer suppression 5,7,10 . The apoptotic cells are fragmented and removed by phagocytosis.…”

Section: Introductionmentioning

confidence: 99%

In Vitro-Screening for Cancer-Suppressive Effect of Food Components

Kobori

2003

JARQ

Self Cite

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Some diets have been shown to be correlated with the development and progression of cancer. In contrast, many kinds of foods are expected to prevent the development and progression of cancer. To identify such cancer-preventive food components and elucidate their cancer-preventive functions, we investigated important anti-cancer effects, that is, growth-inhibitory and apoptosis-inducing effects of some food and food components on cancer cells. Among the vegetables studied, bitter gourd was the most effective in inhibiting the growth of HL60 human leukemia cells. Bitter gourd extract induced apoptosis in the cells. The bitter gourd extract also inhibited growth and induced apoptosis in B16 mouse melanoma 4A5 cells, whereas it inhibited the growth of BALBc/3T3 mouse fibroblast cells only to a small extent. Berries are rich sources of the naturally occurring phenolic pigments anthocyanins, which show a potent antioxidant activity. Bilberry ethanol extract strongly inhibited the growth of HL60 and HCT116 human colon carcinoma cells, and also induced apoptosis in HL60 cells but not in HCT116 cells. Of the berry extracts tested, that from bilberry contained the largest amount of phenolic compounds, including anthocyanins, and exhibited the highest DPPH radical-scavenging activity. The anthocyanidins, delphinidin and malvidin, inhibited cell growth and induced apoptosis in HL60 cells. Delphinidin, but not malvidin inhibited the HCT116 cell growth. These effects of food components probably contribute to cancer prevention.

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Phloretin-induced Apoptosis in B16 Melanoma 4A5 Cells and HL60 Human Leukemia Cells

Cited by 71 publications

References 30 publications

Synergistic inhibition of colon cancer cell growth by a combination of atorvastatin and phloretin

Synergistic inhibition of colon cancer cell growth by a combination of atorvastatin and phloretin

Evaluation of anti–apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide

In Vitro-Screening for Cancer-Suppressive Effect of Food Components

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