Phloem-Conducting Cells in Haustoria of the Root-Parasitic Plant Phelipanche aegyptiaca Retain Nuclei and Are Not Mature Sieve Elements

Ekawa, Minako; Aoki, Koh

doi:10.3390/plants6040060

Cited by 17 publications

(8 citation statements)

References 35 publications

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“…Infection assay with P. aegyptiaca was performed according to [ 63 ]. The seeds were surface sterilized, allowed to imbibe on filter paper for 7 days at 25 °C, and then cultured on MS medium containing 3% sucrose and 0.8% agar for 4 days.…”

Section: Methodsmentioning

confidence: 99%

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Hasegawa

Tsutsumi

Fukushima

et al. 2018

IJMS

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Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants, such as Phelipanche spp., infect tomato roots and cause economic damage in Europe and North Africa—hence why resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the “targeting induced local lesions in genomes” (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.

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Section: Methodsmentioning

confidence: 99%

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Hasegawa

Tsutsumi

Fukushima

et al. 2018

IJMS

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“…Auxin flow is critical for xylem continuity and determines the polarity of the haustorium to allow its function as a root, absorbing water and solutes [ 73 ]. In parallel with the formation of xylem-xylem connections, depending on the parasite species, there is the development of sieve elements in haustoria [ 74 ]. Some holoparasites such as Orobanche/Phelipanche species develop direct symplastic connections through plasmodesmata [ 75 ].…”

Section: Infection By Parasitic Weedsmentioning

confidence: 99%

Management of Infection by Parasitic Weeds: A Review

Fernández‐Aparicio

Delavault

Timko

2020

Plants

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Parasitic plants rely on neighboring host plants to complete their life cycle, forming vascular connections through which they withdraw needed nutritive resources. In natural ecosystems, parasitic plants form one component of the plant community and parasitism contributes to overall community balance. In contrast, when parasitic plants become established in low biodiversified agroecosystems, their persistence causes tremendous yield losses rendering agricultural lands uncultivable. The control of parasitic weeds is challenging because there are few sources of crop resistance and it is difficult to apply controlling methods selective enough to kill the weeds without damaging the crop to which they are physically and biochemically attached. The management of parasitic weeds is also hindered by their high fecundity, dispersal efficiency, persistent seedbank, and rapid responses to changes in agricultural practices, which allow them to adapt to new hosts and manifest increased aggressiveness against new resistant cultivars. New understanding of the physiological and molecular mechanisms behind the processes of germination and haustorium development, and behind the crop resistant response, in addition to the discovery of new targets for herbicides and bioherbicides will guide researchers on the design of modern agricultural strategies for more effective, durable, and health compatible parasitic weed control.

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“…Infection assay with Phelipanche aegyptiaca was performed according to [63]. The seeds were surface sterilized, allowed to imbibe on filter paper for 7 days at 25°C, and then cultured on MS medium containing 3% sucrose and 0.8% agar for 4 days.…”

Section: Root Infection With Phelipanche Aegyptiacamentioning

confidence: 99%

Low infection of Phelipanche aegyptiaca in Micro-Tom mutants deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Hasegawa¹,

Tsutsumi²,

Fukushima³

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Strigolactones (SLs), a group of plant hormones, induce germination of root-parasitic plants and inhibit shoot branching in many plants. Shoot branching is an important trait that affects the number and quality of flowers and fruits. Root-parasitic plants such as Phelipanche spp. infect tomato roots and cause economic damage in Europe and North Africa. Thus, resistant tomato cultivars are needed. In this study, we found carotenoid cleavage dioxygenase 8-defective mutants of Micro-Tom tomato (slccd8) by the "targeting induced local lesions in genomes" (TILLING) method. The mutants showed excess branching, which was suppressed by exogenously applied SL. Grafting shoot scions of the slccd8 mutants onto wild-type (WT) rootstocks restored normal branching in the scions. The levels of endogenous orobanchol and solanacol in WT were enough detectable, whereas that in the slccd8 mutants were below the detection limit of quantification analysis. Accordingly, root exudates of the slccd8 mutants hardly stimulated seed germination of root parasitic plants. In addition, SL deficiency did not critically affect the fruit traits of Micro-Tom. Using a rhizotron system, we also found that Phelipanche aegyptiaca infection was lower in the slccd8 mutants than in wild-type Micro-Tom because of the low germination. We propose that the slccd8 mutants might be useful as new tomato lines resistant to P. aegyptiaca.

show abstract

Phloem-Conducting Cells in Haustoria of the Root-Parasitic Plant Phelipanche aegyptiaca Retain Nuclei and Are Not Mature Sieve Elements

Cited by 17 publications

References 35 publications

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

Management of Infection by Parasitic Weeds: A Review

Low infection of Phelipanche aegyptiaca in Micro-Tom mutants deficient in CAROTENOID CLEAVAGE DIOXYGENASE 8

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