Pheromone receptor of the globally invasive quarantine pest of the palm tree, the red palm weevil (<i>Rhynchophorus ferrugineus</i>)

Antony, Binu; Johny, Jibin; Montagné, Nicolas; Jacquin‐Joly, Emmanuelle; Capoduro, Rémi; Cali, Khasim; Persaud, Krishna; Al-Saleh, M. A.; Pain, Arnab

doi:10.1111/mec.15874

Cited by 33 publications

(32 citation statements)

References 54 publications

(122 reference statements)

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“…To our knowledge, the functional characterization of ORs is relatively poorly understood in coleopterans. Only several ORs of M. caryae (Mitchell et al, 2012), I. typographus (Hou et al, 2020;Yuvaraj et al, 2021), and R. ferrugineus (Antony et al, 2021) are broadly known for aggregation pheromone receptors of beetles. Besides, two ORs of I. typographus specifically responding to host volatiles (Hou et al, 2020) and one OR of Holotrichia parallela responding to three volatiles released by the host tree have been reported (Wang et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The air was filtered through activated charcoal, and the entry flow to the olfactometer was 1.2 L/min controlled by using a vacuum pump. RPW was starved overnight, and the experiment was carried out during daytime between 9:00 and 17:00 h at a temperature of 27 • C ± 1 • C and a humidity of 75 ± 5% (Antony et al, 2021). During the experiment, the Y-tube olfactometer was washed with ethanol and heated every time, and the compound position was changed when every 10 adults were tested.…”

Section: Behavioral Experimentsmentioning

confidence: 99%

“…We chose the initial candidate RferOrs that belong to Group 2 family in beetle species according to previous studies (Antony et al, 2016(Antony et al, , 2021Mitchell et al, 2020). The phylogenetic trees were constructed with four beetle species including R. ferrugineus (Rfer, red), M. caryae (Mc, pink), I. typographus (Ityp, dark), and D. ponderosae (Dpon, blue) and analyzed by using the maximum-likelihood method (Figure 1A).…”

Section: Characteristics Of the Seven Ors In The Rfer-specific Cladementioning

confidence: 99%

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Non-palm Plant Volatile α-Pinene Is Detected by Antenna-Biased Expressed Odorant Receptor 6 in the Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Jia

et al. 2021

Front. Physiol.

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The majority of insects rely on a highly complex and precise olfactory system to detect various volatile organic compounds released by host and non-host plants in environments. The odorant receptors (ORs) are considered to play an important role in odor recognition and the molecular basis of ORs, particularly in coleopterans they are relatively poorly understood. The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae), is one of the most destructive pests of the global palm industry. Although feeding and egg oviposition behaviors of RPW can be repelled by some non-palm plant volatiles, such as α-pinene, geraniol, or 1-octen-3-ol, there is limited understanding of how RPW recognizes the non-host plant volatiles. In this study, three candidate RferOrs were identified from the Rfer-specific clade, and the tissue expression analysis used was mainly expressed in the antennae of both sexes. Functional characterization of RferOr6, RferOr40, and RferOr87 was analyzed by using the Xenopus oocyte expression system, and the results indicated that RferOr6/RferOrco was narrowly tuned to α-pinene. The behavioral experiment showed that α-pinene at the concentrations of 10 and 100 μg/μl can cause a significantly repelled behavioral response of RPW. In conclusion, this study reveals that RferOr6 is an antenna-biased expressed OR used by RPW to detect the volatile compound α-pinene in non-palm plants, and our results provide a foundation for further in vivo functional studies of Or6 in RPW, including in vivo knockout/knockdown and feeding/ovipositing behavioral studies of RPW and further pest control.

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Section: Discussionmentioning

confidence: 99%

Section: Behavioral Experimentsmentioning

confidence: 99%

Section: Characteristics Of the Seven Ors In The Rfer-specific Cladementioning

confidence: 99%

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Non-palm Plant Volatile α-Pinene Is Detected by Antenna-Biased Expressed Odorant Receptor 6 in the Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Jia

et al. 2021

Front. Physiol.

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“…The molecular basis of its chemoreception has been a center of research attention because of its potential for improving pheromone/kairomone bait and biosensors that help the early detection of this pest and mass trapping. Previous research included the identification of chemosensory-related genes [ 58 , 59 , 60 , 64 ], functional analysis of odorant binding protein and a conserved olfactory co-receptor [ 62 ], and recently identification of the aggregation pheromone receptor [ 63 ]. Our study contributes to the understanding of the complex nature of this beetle’s chemosensory function by characterizing all the gustatory receptor genes used for gustation—an under-researched area.…”

Section: Discussionmentioning

confidence: 99%

“…Expressing RferOR1 in Drosophila olfactory neurons shows that this receptor is narrowly tuned to the aggregation pheromone components ferrugineol and ferrugineone. Disrupting this gene using RNAi significantly reduces beetles’ responses to the aggregation pheromone [ 63 ]. Recently, genome data of the R. ferrugineus has become publicly available.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of the Gustatory Receptor Gene Family of the Invasive Pest, Red Palm Weevil, Rhynchophorus ferrugineus (Olivier, 1790)

Engsontia

Satasook

2021

Insects

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The red palm weevil (Rhynchophorus ferrugineus) is a highly destructive pest of oil palm, date, and coconut in many parts of Asia, Europe, and Africa. The Food and Agriculture Organization of the United Nations has called for international collaboration to develop a multidisciplinary strategy to control this invasive pest. Previous research focused on the molecular basis of chemoreception in this species, particularly olfaction, to develop biosensors for early detection and more effective bait traps for mass trapping. However, the molecular basis of gustation, which plays an essential role in discriminating food and egg-laying sites and chemical communication in this species, is limited because its complete gustatory receptor gene family still has not been characterized. We manually annotated the gene family from the recently available genome and transcriptome data and reported 50 gustatory receptor genes encoding 65 gustatory receptors, including 7 carbon dioxide, 9 sugar, and 49 bitter receptors. This study provides a platform for future functional analysis and comparative chemosensory study. A better understanding of gustation will improve our understanding of this species’ complex chemoreception, which is an important step toward developing more effective control methods.

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Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

Xiu-lin

Chen

et al. 2021

Arch Insect Biochem Physiol

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The olfactory system must detect and discriminate various semiochemicals in the environment. In response to such diversity, insects have evolved a family of odorant‐gated ion channels composed of a common receptor (coreceptor, Orco) and a ligand‐binding tuning odorant receptor (OR) that confers odour specificity. This study aims to examine the expression pattern of Orco gene of Grapholita molesta (GmolOrco) and to elucidate the role of GmolOrco in detecting G. molesta sex pheromone and green leaf volatiles by using gene silencing via RNA interference (RNAi) coupled antennal electrophysiological (EAG). Multiple sequence alignment showed that GmolOrco shared high sequence similarities with the Orco ortholog of lepidopterans. The results of real‐time quantitative PCR detection demonstrated that GmolOrco was predominantly expressed in adult antennae and had the highest expression quantity in adult period among the different developmental stages. Compared with the noninjected controls, GmolOrco expression in GmolOrcodouble‐stranded RNA (dsRNA)‐injected males was reduced to 39.92% and that in females was reduced to 40.43%. EAG assays showed that the responses of GmolOrco‐dsRNA injected males to sex pheromones (Z)‐8‐dodecenyl acetate (Z8‐12:OAc) and (Z)‐8‐dodecenyl alcohol (Z8‐12:OH) were significantly reduced, and the GmolOrco‐dsRNA‐injected female to green leaf volatile (Z)‐3‐hexenyl acetate also significantly declined. We inferred that Orco‐mediated olfaction was different in male and female G. molesta adults and was mainly involved in recognizing the sex pheromones released by female moths.

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Pheromone receptor of the globally invasive quarantine pest of the palm tree, the red palm weevil (Rhynchophorus ferrugineus)

Cited by 33 publications

References 54 publications

Non-palm Plant Volatile α-Pinene Is Detected by Antenna-Biased Expressed Odorant Receptor 6 in the Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Non-palm Plant Volatile α-Pinene Is Detected by Antenna-Biased Expressed Odorant Receptor 6 in the Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Genome-Wide Identification of the Gustatory Receptor Gene Family of the Invasive Pest, Red Palm Weevil, Rhynchophorus ferrugineus (Olivier, 1790)

Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae)

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