Phenylurethane herbicides: Inhibitors of changes in metabolic state

Mann, Jay D.; Cota-Robles, E. H.; Yung, Kung-Hing; Pu, Minn; Haid, Heidi

doi:10.1016/0005-2787(67)90593-x

Cited by 27 publications

(13 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it may serve as a qualitative general model system for cytokinin-binding proteins (including the cytokinin receptor) since it binds all purine and nonpurine cytokinins tested as well as a structurally diverse range of ligands related to those recognized by other cytokinin-binding proteins. For example, the carbanilate herbicides such as CIPC can delay senescent degreening (20) and are antimitotic agents (13,14,16,21) that can inhibit cytokinin-induced cell division (16). The binding of the carbanilate esters tested to CBP (Table II and III) is consistent with the hypothesis that these antimitotics act antagonistically at the level of the actual cytokinin receptor, inasmuch as the receptor and CBP clearly have overlapping ligand specificities.…”

supporting

confidence: 70%

Ligand Specificity of a High Affinity Cytokinin-binding Protein

Polya

Bowman²

1979

Plant Physiol.

View full text Add to dashboard Cite

A soluble cytokinin-binding protein from wheat germ that has a high affinity for a range of purine cytokinins also interacts with a variety of nonpurine compounds that can affect cytokinin-modified processes in aninal or plant cels or which bind to proteins known to interact with certain cytokinins. A variety of structuraly disprate compounds which inhibit chloroplast photosystem 11 activity (including pbenylurea, carbanilate, and alkylamino-2-chloro-syn-triazine compounds) displace High affinity cytokinin-binding proteins are present in the ribosomal and postribosomal supernatant fractions from centrifugal fractionation of wheat germ homogenates (9, 10). Fox and Erion (10) have resolved an approximately 100,000 dalton high affinity cytokinin-binding protein (CBF-1) from the ribosomal fraction from wheat germ. They have also resolved a very similar protein (probably identical to CBF-1), an approximately 30,000 dalton high affinity cytokinin-binding protein (CBF-2) and a high mol wt low affinity cytokinin-binding protein (CBF-3) from the postribosomal supernatant fraction from wheat germ (10). Polya and Davis (25) Equilibrium Dialysis. Equilibrium dialysis was performed using 10-cm-long sacs ofsize 8/32 dialysis tubing (Visking Co., Chicago) enclosing I ml of a solution containing 0.5 M (NH4)2SO4, 50 mM Tris-HCl (pH 8.0), 0.1% (v/v) 2-mercaptoethanol, and 3.6 mg CBP. Dialysis sacs were suspended in 40 ml of a solution containing 0.5 M (NH4)2S04, 50 mm Tris-HCl (pH 8.0), 0.1% (v/v)

show abstract

supporting

confidence: 70%

Ligand Specificity of a High Affinity Cytokinin-binding Protein

Polya

Bowman²

1979

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…The effects of molinate on the metabolism of protein, on the other hand, may also interfere with the formation of some special protein such as C-phycocyanin or its link to other component parts, resulting in the decrease in biliprotein content (Table 3) and the passivation of some functional proteins related to cell division and electron transport in photosynthesis (Mattoo et al, 1984). It is speculated that molinate imparts its toxicity by inhibiting the ability to synthesize a specific enzyme (Mann et al, 1967) and by complexing with the Y-33 protein in complex B of photosystem II in thylakoidal membranes, thereby leading to a block in the electron transport chain which makes excitation energy unable to be effectively transported to central pigment P680 in thylakoidal membranes. A similar explanation was suggested by Schulz et al (1990) for the effect of triazine herbicides on higher plants.…”

Section: Discussionmentioning

confidence: 98%

Effects of the Herbicide Molinate on Mixotrophic Growth, Photosynthetic Pigments, and Protein Content ofAnabaena sphaericaunder Different Light Conditions

Yan

1997

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

“…Although microtubules, or microtubule associated material, are implicated as the target of IPC action in all of these studies, the wide variation in concentration of IPC used, the different assay methods, and the variable responses in these widely diverse organisms make it difficult to propose a uniform mode of action for IPC . In addition, earlier reports that the 3-chlorophenyl derivative of IPC, CIPC, inhibits protein synthesis in several plant systems (Mann et al ., 1965 ;1967) and the hypothesis that IPC and CIPC may inhibit messenger RNA transcription and hence, new protein synthesis (Mann et al ., 1967 ;Keitt, 1967) suggested that some of the responses listed above (e .g ., inhibition of cilia regeneration and flagellum morphogenesis) may be due to inhibition of protein synthesis, including the synthesis of microtubule protein .…”

Section: Discussionmentioning

confidence: 99%

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

Brown

Bouck

1974

The Journal of Cell Biology

View full text Add to dashboard Cite

The role of microtubules and microtubule nucleating sites in the unicell, Ochromonas has been examined through the use of two mitotic inhibitors, isopropyl N-phenylcarbamate (IPC) and isopropyl N-3-chlorophenyl carbamate (CIPC) . Although IPC and CIPC have little or no effect on intact microtubules, the assembly of three separate sets of microtubules in Ochromonas has been found to be differentially affected by IPC and CIPC . The assembly of flagellar microtubules after mechanical deflagellation is partially inhibited ; the reassembly of rhizoplast microtubules after pressure depolymerization is totally inhibited (however, macrotubules may form at the sites of microtubule initiation or elsewhere) ; and, the reassembly of the beak set of microtubules after pressure depolymerization may be unaffected although similar concentrations of IPC and CICP completely inhibit microtubule regeneration on the rhizoplast . These effects on microtubule assembly, either inhibitory or macrotubule inducing, are fully reversible . The kinetics of inhibition and reversal are found to be generally similar for both flagellar and cell shape regeneration . Incorporation data suggest that neither IPC nor CIPC has significant effects on protein synthesis in short term experiments . Conversely, inhibiting protein synthesis with cycloheximide has little effect on microtubule regeneration when IPC or CIPC is removed . Although the exact target for IPC and CIPC action remains uncertain, the available evidence suggests that the microtubule protein pool or the microtubule nucleating sites are specifically and reversibly affected . Comparative experiments using the mitotic inhibitor colchicine indicate some similarities and differences in its mode of action with respect to that of IPC and CIPC on assembly and disassembly of microtubules in these cells .In the first two papers of this series (Bouck and ently independent sets of cytoplasmic microBrown, 1973 ; Brown and Bouck, 1973) evidence tubules which (a) differ from each other in sensiwas presented that the characteristic asymmetric tivity to the microtubule depolymerizing agents shape of Ochromonas is mediated by two appar-colchicine and hydrostatic pressure, (b) are 514

show abstract

Phenylurethane herbicides: Inhibitors of changes in metabolic state

Cited by 27 publications

References 10 publications

Ligand Specificity of a High Affinity Cytokinin-binding Protein

Ligand Specificity of a High Affinity Cytokinin-binding Protein

Effects of the Herbicide Molinate on Mixotrophic Growth, Photosynthetic Pigments, and Protein Content ofAnabaena sphaericaunder Different Light Conditions

MICROTUBULE BIOGENESIS AND CELL SHAPE IN OCHROMONAS

Contact Info

Product

Resources

About