Phenylethanoid glycosides accumulation and antiradical activity of fractionated extracts of Plantago ovata Forssk. callus cultures lines

Abstract

The main phenylethanoid glycosides in the Plantago genus are acteoside (verbascoside) and plantamajoside, compounds with broad biological effects. This is a report on Plantago ovata callus induction, proliferation and establishment as well as the content of those phenylethanoids in that cell biomass. In the experimental studies, callus initiated from various seedling explants (roots, hypocotyls and leaves) was cultured on MS (Murashige-Skoog) media augmented with 2,4-D (2,4-dichloroacetic acid) and KIN (kinetin) or NAA (α-naphthaleneacetic acid) and BAP (6-benzylaminopurine). Callus proliferating on MS without NH4NO3 (ammonium nitrate) supplemented with 2,4-D (1.0 mg/l) and KIN (0.5 mg/l or 1.0 mg/l) turned out to be a good growth system for biomass production—mean increase of fresh weigh calculated on three following passages was 9.1 ± 1.8. The phytochemical analyses and antiradical DPPH (1,1-diphenyl-2-picryl-hydrazyl) tests revealed that the antioxidant activity is due to the presence of phenylethanoid glycosides. The quantitative screening of the callus extract by TLC (thin-layer chromatography) video densitometric method showed the highest content of acteoside (9.58 ± 0.75 mg/g dry weight) in root-derived and plantamajoside (8.15 ± 0.81 mg/g d.w.) in hypocotyl-derived callus biomass. In in vitro redifferentiated cultures of P. ovata, compounds with a demonstrated therapeutic effect, can be obtained in a manner that is completely independent of cultivation or harvesting from the wild. Graphical abstract

Improvement of Bioactive Polyphenol Accumulation in Callus of Salvia atropatana Bunge

“…The concentration of verbascoside has also been found to range from 4.3 mg/g DW in callus of a leaf-derived line of Plantago ovata Forssk. to 9.6 mg/g DW in a root-derived line [33]. Since the secondary metabolite accumulation in plants is genotype-specific, the selection of high-producing culture lines is an important step in obtaining valuable material which could represent a beneficial source of metabolites for commercial application [3].…”