Phenylethanoid and Phenylmethanoid Glycosides from the Leaves of Ligustrum robustum and Their Bioactivities

Lu, Shi-Hui; Zuo, Haojiang; Huang, Jing; Chen, Ran; Pan, Jiaping; Li, Xiuxia

doi:10.3390/molecules27217390

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…In addition, among the harmful effects of fatigue, oxidative stress and inflammation have also been observed [ 60 ]. Our findings enable deeper insight into the therapeutic potential of alternating the BALF microbiota, especially Vibrio , to alleviate fatigue in patients with silicosis [ 61 – 63 ]. However, the relationship between BALF Vibrio , fatigue, and haemoglobin remains to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Microbiota and mycobiota in bronchoalveolar lavage fluid of silicosis patients

Xie

Zhang

Gao

et al. 2023

J Occup Med Toxicol

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Background The contribution of bronchoalveolar lavage fluid (BALF) microbiota and mycobiota to silicosis has recently been noticed. However, many confounding factors can influence the accuracy of BALF microbiota and mycobiota studies, resulting in inconsistencies in the published results. In this cross-sectional study, we systematically investigated the effects of “sampling in different rounds of BALF” on its microbiota and mycobiota. We further explored the relationship between silicosis fatigue and the microbiota and mycobiota. Methods After obtaining approval from the ethics board, we collected 100 BALF samples from 10 patients with silicosis. Demographic data, clinical information, and blood test results were also collected from each patient. The characteristics of the microbiota and mycobiota were defined using next-generation sequencing. However, no non-silicosis referent group was examined, which was a major limitation of this study. Results Our analysis indicated that subsampling from different rounds of BALF did not affect the alpha- and beta-diversities of microbial and fungal communities when the centrifuged BALF sediment was sufficient for DNA extraction. In contrast, fatigue status significantly influenced the beta-diversity of microbes and fungi (Principal Coordinates Analysis, P = 0.001; P = 0.002). The abundance of Vibrio alone could distinguish silicosis patients with fatigue from those without fatigue (area under the curve = 0.938, 95% confidence interval [CI] 0.870–1.000). Significant correlations were found between Vibrio and haemoglobin levels (P < 0.001, ρ = -0.64). Conclusions Sampling in different rounds of BALF showed minimal effect on BALF microbial and fungal diversities; the first round of BALF collection was recommended for microbial and fungal analyses for convenience. In addition, Vibrio may be a potential biomarker for silicosis fatigue screening.

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Section: Discussionmentioning

confidence: 99%

Microbiota and mycobiota in bronchoalveolar lavage fluid of silicosis patients

Xie

Zhang

Gao

et al. 2023

J Occup Med Toxicol

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“…In the previous phytochemical studies [ 2 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ], about 90 chemical constituents, including trans - p -hydroxycinnamic acid, 34 trans - p -hydroxycinnamic acid esters ( Figure 1 ), flavonoid glycosides, lignan glycosides, and other compositions, were isolated and identified from the leaves of L. robustum .…”

Section: Introductionmentioning

confidence: 99%

“…In our previous biological investigations [ 14 , 15 , 16 , 17 ], the chemical compositions isolated from the leaves of L. robustum were tested, and the results showed that (1) trans - p -hydroxycinnamic acid displayed stronger α -glucosidase inhibitory activity than the positive control acarbose; (2) trans - p -hydroxycinnamic acid and several trans - p -hydroxycinnamic acid esters displayed no weaker fatty acid synthase (FAS) inhibitory activities than the positive control orlistat; (3) trans - p -hydroxycinnamic acid and a lot of trans - p -hydroxycinnamic acid esters revealed stronger 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) ammonium salt (ABTS) radical scavenging effects than the positive control L-(+)-ascorbic acid and revealed moderate α-amylase inhibitory activities. What is more, trans - p -hydroxycinnamic acid esters might be hydrolyzed with catalysis of carboxylesterase or gastric acid in vivo [ 18 , 19 ] and release trans - p -hydroxycinnamic acid, meaning that trans - p -hydroxycinnamic acid esters are the prodrugs of trans - p -hydroxycinnamic acid.…”

Section: Introductionmentioning

confidence: 99%

A New HPLC-UV Method Using Hydrolyzation with Sodium Hydroxide for Quantitation of Trans-p-Hydroxycinnamic Acid and Total Trans-p-Hydroxycinnamic Acid Esters in the Leaves of Ligustrum robustum

Lu,

Liang,

Nong

et al. 2023

Molecules

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Trans-p-hydroxycinnamic acid and its esters in the leaves of Ligustrum robustum might be a new resource to prevent diabetes and its complications. In the present study, a new HPLC-UV method using hydrolyzation with sodium hydroxide for quantitation of trans-p-hydroxycinnamic acid and total trans-p-hydroxycinnamic acid esters in the leaves of L. robustum was developed, since it was difficult and troublesome to analyze no less than 34 trans-p-hydroxycinnamic acid esters by usual HPLC. The extract of L. robustum was hydrolyzed with sodium hydroxide at 80 °C for 2 h, and then, hydrochloride was added. HPLC analysis was performed in reverse phase mode using a C-18 column, eluting with methanol-0.1% acetic acid aqueous solution (40:60, v/v) in isocratic mode at a flow rate of 1.0 mL·min−1 and detecting at 310 nm. The linear range for trans-p-hydroxycinnamic acid was 11.0–352.0 μg·mL−1 (r2 = 1.000). The limit of detection and limit of quantification were 2.00 and 6.07 μg·mL−1, respectively. The relative standard deviations of intra-day and inter-day variabilities for trans-p-hydroxycinnamic acid were less than 2%. The percentage recovery of trans-p-hydroxycinnamic acid was 103.3% ± 1.1%. It is the first HPLC method using hydrolyzation for quantification of many carboxylic esters. Finally, the method was used successfully to determine trans-p-hydroxycinnamic acid and total trans-p-hydroxycinnamic acid esters in various extracts of the leaves of L. robustum. The 60–70% ethanol extracts of L. robustum showed the highest contents of free trans-p-hydroxycinnamic acid (3.96–3.99 mg·g−1), and the 50–80% ethanol extracts of L. robustum displayed the highest contents of total trans-p-hydroxycinnamic acid esters (202.6–210.6 mg·g−1). The method can be applied also to the quality control of the products of L. robustum.

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Comment on the “Eco-friendly and efficient extraction of polyphenols from Ligustrum robustum by deep eutectic solvent assisted ultrasound”

Frański

2024

Food Chemistry

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Phenylethanoid and Phenylmethanoid Glycosides from the Leaves of Ligustrum robustum and Their Bioactivities

Cited by 4 publications

References 24 publications

Microbiota and mycobiota in bronchoalveolar lavage fluid of silicosis patients

Microbiota and mycobiota in bronchoalveolar lavage fluid of silicosis patients

A New HPLC-UV Method Using Hydrolyzation with Sodium Hydroxide for Quantitation of Trans-p-Hydroxycinnamic Acid and Total Trans-p-Hydroxycinnamic Acid Esters in the Leaves of Ligustrum robustum

Comment on the “Eco-friendly and efficient extraction of polyphenols from Ligustrum robustum by deep eutectic solvent assisted ultrasound”

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