Phenylethanoid and iridoid glycosides production in Rehmannia elata N.E.Brown ex Prein. in vitro shoot cultures and their biological activity

Piątczak, Ewelina; Kuźma, Łukasz; Kozłowska, Weronika; Lisiecki, Paweł; Szemraj, Magdalena; Płachno, Bartosz J.; Gonciarz, Weronika; Chmiela, Magdalena; Matkowski, Adam; Zielińska, Sylwia

doi:10.1016/j.indcrop.2020.113050

Cited by 9 publications

(3 citation statements)

References 53 publications

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“…24 Similar effects were found in Hypericum perforatum and Rehmannia elata. 25,26 In the current study, changing light quality revealed a marked effect on the accumulation of secondary metabolites in buckwheat shoots. Initial culture trials showed that white and blue lights are superior to other lights in accumulation of rutin.…”

Section: Discussioncontrasting

confidence: 99%

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Gabr

Fayek

Mahmoud³

et al. 2022

ACS Omega

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Common buckwheat ( Fagopyrum esculentum Moench) seeds are important nutritious grains that are widely spread in several human food products and livestock feed. Their health benefits are mainly due to their bioactive phenolic compounds, especially rutin and quercetin, which have a positive impact on heart health, weight loss, and diabetes management. In this study, we evaluated different media and light treatments for the in vitro cultures of common buckwheat (CB) in order to find the most optimum one producing the highest yield with the highest purity of these compounds. The subcultured treated samples included in this study were shoots, leaves, stems, hairy roots, and calli. From the several treated samples and under different light stress conditions, the best production was achieved by growing the shoots of common buckwheat in hormone-free media containing activated charcoal and exposing to blue light, attaining 4.3 mg and 7.0 mg/g of extracts of rutin and quercetin, respectively, compared to 3.7 mg of rutin/g of extract and traces of quercetin in the seeds of CB. Continuous multiplication of CB shoots in the media containing charcoal and different concentrations of kinetin produced an extract with 161 mg/g of rutin and 26 mg/g of quercetin with an almost 20-fold increase in rutin content. The rutin content under these conditions reached up to 16% w/w of the extract. The hairy root cultures of the leaves exposed to red light showed a significantly high yield of quercetin attaining 10 mg/g of extract. Large-scale production of CB shootlets under the best conditions were carried out, which enabled the isolation of pure quercetin and rutin using a simple chromatographic procedure. The identity and purity of the isolated compounds were confirmed through NMR and HPLC analyses.

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Section: Discussioncontrasting

confidence: 99%

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Gabr

Fayek

Mahmoud³

et al. 2022

ACS Omega

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“…In addition, light quality can be applied for the elicitation of vital phytochemicals with pharmaceutical importance. Recently, the application of light quality for the enhancement of secondary metabolites with medicinal importance in a controlled environment such as plant factories, green houses, and in vitro culture environment is increasing ( Im Chung et al, 2020 ; Park et al, 2020 ; Pia̧tczak et al, 2020 ; Tohidi et al, 2020 ). Red and blue wavelengths (650 and 450 nm, respectively) are often associated with the enhancement of physiological processes in plants ( Kim et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement of in vitro Growth in Scrophularia kakudensis—A Potential Medicinal Plant

et al. 2021

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The current study has determined the effect of red and blue lights on the enhancement of growth, antioxidant property, phytochemical contents, and expression of proteins in Scrophularia kakudensis. In vitro-grown shoot tip explants of S. kakudensis were cultured on the plant growth regulator-free Murashige and Skoog (MS) medium and cultured under the conventional cool white fluorescent lamp (control), blue light-emitting diodes (LED) light, or red LED light. After 4 weeks, growth, stomatal ultrastructure, total phenols and flavonoids, activities of antioxidant enzymes, and protein expressions were determined. Interestingly, blue or red LED treatment increased the shoot length, shoot diameter, root length, and biomass on comparison with the control. In addition, the LED treatments enhanced the contents of phytochemicals in the extracts. The red LED treatment significantly elicited the accumulation of flavonoids in comparison with the control. In accordance with the secondary metabolites, the LED treatments modulated the activities of antioxidant enzymes. Moreover, the proteomic insights using two-dimensional gel electrophoresis system revealed the proteins involved in transcription and translation, carbohydrate mechanism, post-translational modification, and stress responses. Taken together, the incorporation of blue or red LED during in vitro propagation of S. kakudensis can be a beneficial way to increase the plant quality and medicinal values of S. kakudensis.

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“…For SEM, the hairy roots fragments were prepared according to previously published methodology (Piątczak et al 2020) using a Hitachi S-4700 scanning electron microscope (Hitachi, Tokyo, Japan), housed in the Institute of Geological Sciences, Jagiellonian University in Kraków).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Upscaling biomass production of rosmarinic acid-rich hairy root cultures of Agastache rugosa

Kozłowska

Piątczak

Kolniak‐Ostek

et al. 2023

Preprint

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Agastache rugosa (Fisch. & C.A.Mey.) Kuntze known as Korean Mint is a medicinal and aromatic plant containing rosmarinic acid as a major bioactive polyphenol but its content in this herbal drug is variable. Plant in vitro culture is one of the approaches used to overcome the complexity of environmental factors influencing phytochemical profiles in medicinal plants. In this study, hairy root cultures of A. rugosa were established through the infection of Rhizobium rhizogenes. Four hairy roots lines were selected on the basis of biomass production in liquid media. Transformation was confirmed by PCR using rolC specific primers. The hairy roots were successfully cultured in 300 mL conical flasks and scaled-up using three bioreactor types (nutrient sprinkle bioreactor – NSB and two modular temporary immersion systems – RITA® and Plantform®). The UPLC analysis of A. rugosa transformed roots methanol extracts showed the presence of 24 polyphenolic compounds with the predominance of rosmarinic acid (RA), which level ranged between 3.82 and 9.16 mg/g of dry weight. Nineteen compounds were identified in hairy roots growing in NSB system, 9 of them were identified in roots cultured in RITA® or Plantform® and 7 compounds were identified in roots from Erlenmeyer flasks. The A. rhizogenes infection (strain A4) was found to be an effective method of hairy root culture establishment of A. rugosa.

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Phenylethanoid and iridoid glycosides production in Rehmannia elata N.E.Brown ex Prein. in vitro shoot cultures and their biological activity

Cited by 9 publications

References 53 publications

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Physiological and Proteomic Insights Into Red and Blue Light-Mediated Enhancement of in vitro Growth in Scrophularia kakudensis—A Potential Medicinal Plant

Upscaling biomass production of rosmarinic acid-rich hairy root cultures of Agastache rugosa

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