Phenylene–ethynylene trication as an efficient fluorescent signal transducer in an aptasensor for potassium ion

Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor

“…A linear calibration curve was attained between fluorescence intensity changes and the logarithm of K + ions concentrations (R 2  = 0.9810). The limit of detection for K + ions was 0.1 nM, which was much lower than the concentration reported in the previous studies 31, 36 . Based on the DNA-AgNCs fluorescence sensing platform, the whole assay procedure can be completed in 10 min.…”

“…A novel DNA sequence of 5′-GGTTGGTGTGGTTGG ATCCCCCCCCCCCC-3′ was synthesized by Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, China). 5′-CCCCCCCCCCCC-3′(dC 12 ) was the scaffold for synthesis of AgNCs and 5′-GGTTGGTGTGGTTGG-3′ was the K + ions aptamer which was widely used in a great deal of researches 30, 31 . A concentration of 20 mM phosphate-buffered (PB) was used throughout the experiments.…”

Versatile G-quadruplex-mediated strategies in label-free biosensors and logic systems

“…[63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79] In 2009, Dong's group first compared the sensitivity of distinct G4 sequences and obtained one optimal colorimetric method for K + determination using an AGRO100 G4-hemin complex and ABTS-H 2 O 2 reaction; 65 meanwhile, Kong and coworkers for the first time proposed the utilization of a G4-specific fluorescent probe, crystal violet (CV, a triphenylmethane dye), to monitor the G4 conformation change induced by K + . K + , Na + and Pb 2+ ) are able to promote the formation of the G4 structure and stabilize its specific conformation, which has been the basis for metal ionresponsive sensing and molecular logic systems.…”

A time-resolved luminescent competitive assay to detect L-selectin using aptamers as recognition elements

“…Aptamer-based FRET sensing systems have been employed for recognizing poisonous cations, e.g. Pb 2þ and Hg 2þ [21,22], K þ [19,23], low molecular weight targets such as cocaine [19,24], adenosine [19] or ATP [25] macromolecular markers such as thrombin [26,27], vascular endothelial growth factor (VEGF) [28], insulin [29], or even whole cancer cells [20]. Noteworthy, an original multifunctional quantum dot/aptamer/drug system was also developed that could simultaneously recognize the prostate specific membrane antigen, visualize prostate cancer cells and finally release an antineoplastic drug, namely doxorubicin [30].…”