Phenylarsine oxide (PAO) induces apoptosis in HepG2 cells<i>via</i>ROS-mediated mitochondria and ER-stress dependent signaling pathways

Huang, Ping; Zhang, Yu Hua; Zheng, Xiaoying; Liu, Yu Jia; Zhang, Hong; Fang, Luo; Zhang, Yi Wen; Yang, Chang; Islam, Khairul; Wang, Chao; Naranmandura, Hua

doi:10.1039/c7mt00179g

Cited by 35 publications

(17 citation statements)

References 33 publications

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“…The change in lipid mobility evoked by PAO and the decrease in the CM amplitude are 382 consistent with the DX-52-1 findings; however PAO is unspecific (25) and will affect many 383 proteins found in stereocilia, which likely explains why the effects on sound-evoked motion 384 and on electromotility differ from those of DX-52-1. 385…”

supporting

confidence: 60%

Radixin modulates stereocilia function and contributes to cochlear amplification

Prasad

Vona

Diñeiro

et al. 2020

Preprint

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The stereocilia of the sensory cells in the inner ear contain high levels of the actin-binding protein radixin, encoded by the RDX gene. Radixin which is associated with mechanotransduction process such as PIP2 is known to be important for hearing but its functional role remains obscure. To determine how radixin influences hearing sensitivity, we used a custom rapid imaging technique to directly visualize stereocilia motion while measuring the amplitude of the electrical potentials produced by sensory cells during acoustic stimulation. Experiments were performed in guinea pigs, where upon blocking radixin, a large decrease in sound-evoked electrical potentials occurred. Despite this decrease other important functional measures, such as electrically induced sensory cell motility and the sound-evoked deflections of stereocilia, showed a minor amplitude increase. This unique set of functional properties alterations demonstrate that radixin is necessary to ensure that the inner ear converts sound into electrical signals at acoustic rates. Radixin is therefore a necessary and important component of the cochlear amplifier, the energy-consuming process that boosts hearing sensitivity by up to 60 dB.

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supporting

confidence: 60%

Radixin modulates stereocilia function and contributes to cochlear amplification

Prasad

Vona

Diñeiro

et al. 2020

Preprint

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“…Endoplasmic reticulum (ER) stress is emerging as a modulator of different pathologies and as an important mechanism contributing to cancer cell death in response to therapeutic agents [15, 16]. In several instances, oxidative stress and the onset of ER stress occur together [17, 18].…”

Section: Resultsmentioning

confidence: 99%

Isoalantolactone induces apoptosis through ROS-mediated ER stress and inhibition of STAT3 in prostate cancer cells

Chen

Liu

et al. 2018

J Exp Clin Cancer Res

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BackgroundProstate cancer is one of the most commonly diagnosed cancers in men worldwide. Currently available therapies for metastatic prostate cancer are only marginally effective. Therefore, new therapeutic agents are urgently needed to improve patient outcome. Isoalantolactone (IATL), an active sesquiterpene naturally present in many vegetables and medicinal plants, is known to induce cell death and apoptosis in various cancer cell lines. Nevertheless, antitumor mechanisms initiated by IATL in cancer cells have not been fully defined.MethodsCell apoptosis and cellular ROS levels were analyzed by flow cytometry. Western blot and qRT-PCR were used to analyze the protein and mRNA levels of indicated molecules, respectively. Nude mice xenograft model was used to test the effects of IATL on prostate cancer cell growth in vivo.ResultsIn this study, we found that IATL dose-dependently inhibited cancer cell growth and induced apoptosis in PC-3 and DU145 cells. Mechanistically, our data found that IATL induced reactive oxygen species (ROS) production, resulting in the activation of endoplasmic reticulum stress pathway and eventually cell apoptosis in prostate cancer cells. IATL also decreased the protein expression levels of p-STAT3 and STAT3, and the effects of IATL were reversed by pretreatment with N-acetyl-L-cysteine (NAC). In vivo, we found that IATL inhibited the growth of prostate cancer xenografts without exhibiting toxicity. Treatment of mice bearing human prostate cancer xenografts with IATL was also associated with induction of ER stress and inhibtion of STAT3.ConclusionIn summary, our results unveil a previously unrecognized mechanism underlying the biological activity of IATL, and provide a novel anti-cancer candidate for the treatment of prostate cancer.

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“…The ROS generation assay was performed as previously described (32). The SGC7901 and BGC823 cells were exposed to various concentrations (0, 10, 20 and 30 µ M) of corilagin with or without NAC for 24 h, washed with PBS, and resuspended in culture medium (without serum) containing 10 µ M DCFH-DA.…”

Section: Methodsmentioning

confidence: 99%

Corilagin induces apoptosis, autophagy and ROS generation in gastric cancer cells in�vitro

Zhang

Tong

et al. 2018

Int J Mol Med

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Corilagin, a unique component of the tannin family, has been identified in several medicinal plants. In previous literature, corilagin exhibited a marked anticancer property in a variety of human cancer cells. However, the biological effects of corilagin on gastric cancer and the mechanisms involved remain to be fully elucidated. In the present study, it was reported that corilagin induced inhibition of cell growth in SGC7901 and BGC823 cells in a concentration-dependent manner. It was found that corilagin exhibited less toxicity towards normal GES-1 cells. Furthermore, the study showed that corilagin induced the apoptosis of gastric cancer cells mainly via activating caspase-8, -9, -3 and poly ADP-ribose polymerase proteins. Simultaneously, it was verified that corilagin triggered autophagy in gastric cancer cells and the inhibition of autophagy improved the activity of corilagin on cell growth suppression. In addition, corilagin significantly increased intracellular reactive oxygen species production, which is important in inhibiting the growth of gastric cancer cells. Finally, it was shown that necroptosis cannot be induced by corilagin-incubation in SGC7901 and BGC823 cell lines. Consequently, these findings indicate that corilagin may be developed as a potential therapeutic drug for gastric cancer.

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Phenylarsine oxide (PAO) induces apoptosis in HepG2 cellsviaROS-mediated mitochondria and ER-stress dependent signaling pathways

Cited by 35 publications

References 33 publications

Radixin modulates stereocilia function and contributes to cochlear amplification

Radixin modulates stereocilia function and contributes to cochlear amplification

Isoalantolactone induces apoptosis through ROS-mediated ER stress and inhibition of STAT3 in prostate cancer cells

Corilagin induces apoptosis, autophagy and ROS generation in gastric cancer cells in�vitro

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