Phenylalanine Ammonia-lyase, Tyrosine Ammonia-lyase, and Lignin in Wheat Inoculated with Erysiphe graminis f. sp. tritici

Green, Norman E.

doi:10.1094/phyto-65-1071

Cited by 52 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In other host-parasite interactions the onset of the induction of putative resistance reactions, e.g. synthesis of phytoalexins (1,14,20) and lignification (10) has been proposed to be critical in determining resistance. However, at present no function can be assigned to the RP's.…”

Section: Discussionmentioning

confidence: 99%

Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus

1985

View full text Add to dashboard Cite

Pairs of susceptible and resistant, near-isogenic cultivars ofHordeum vulgare which differ for the Mla, Mlk and Mlp genes for resistance toErysiphe graminis f. sp.hordei were inoculated with race 3 of this pathogen and patterns of protein synthesis associated with primary infection mapped using pulse-labelling with L-[(35)S]methionine and 2-dimensional electrophoresis. Extraction of proteins with buffer containing detergent revealed the enhanced synthesis of 5 and 8 polypeptides at 25 and 30 h respectively after inoculation of barley carrying the Mla gene (cvMla). The enhanced synthesis of these same polypeptides together with 11 additional polypeptides was observed at 48 h and 72 h after inoculation of barley carrying either the Mlp (cvMlp) or Mlk (cvMlk) genes. The labelling of several major constitutive polypeptides was suppressed in cvMla at 24 h after inoculation; the labelling of six of these polypeptides was also suppressed in both cvMlp and cvMlk but not until 48 and 72 h after inoculation. These results indicate that changes occur in the synthesis of some common polypeptides following infection of cultivars carrying different resistance genes but the timing and extent of these changes varies with the resistance gene in the host.

show abstract

Section: Discussionmentioning

confidence: 99%

Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus

1985

View full text Add to dashboard Cite

show abstract

“…Phenylalanine is suggested to associate with tyrosine. Both metabolites are important in plant defence against biotic stress in phenol synthesis and lignin accumulation which is catalysed by tyrosine ammonia lyase and PAL (Green et al, 1975). Concurrently with phenylalanine and tyrosine, tryptophan is derived from chorismate originates from the shikimate pathway (Wakasa & Ishihara, 2009).…”

Section: Soluble Protein and Amino Acid In Rice Lines Infected By R mentioning

confidence: 99%

Metabolite profiling of sheath blight disease resistance in rice: in the case of positive ion mode analysis by CE/TOF-MS

Suharti

Nose

Zheng

2016

Plant Production Science

View full text Add to dashboard Cite

Rice sheath blight is an important disease caused by Rhizoctonia solani. The resistant and susceptible rice lines (32R and 29S, respectively) showed different responses to R. solani infection in metabolite levels. The aim of this study was to characterize the metabolite levels in rice lines during R. solani infection using capillary electrophoresis equipped with time of flight mass spectrophotometry (CE/TOF-MS) in positive ion mode. Hundred metabolites were identified and classified into six clusters by hierarchical cluster using Mass Profiler Professional software. Changes in metabolite level at inoculated 32R and 29S were mapped on branches of tricarboxylic acid and glycolysis pathway. Volcano plot successfully filtered the metabolites based on fold change and p-value. The volcano plot result showed that 10 metabolites were up and down regulated in inoculated 32R relative to 29S. One metabolite, chlorogenic acid, showed a positive response in 32R. Meanwhile, pipecolic acid showed as the highest magnitude of fold change and p-value significance level in 29S. In addition, eight amino acids; glutamate, γ-aminobutyric acid, glycine, histidine, phenylalanine, serine, tryptophan, and tyrosine showed increase in 29S after R. solani inoculation.

show abstract

“…Activity of PAL was measured as described by Green et al (1975). The assay contained crude extract and 3 mM L-phenylalanine in 50 mM borate buffer, pH 8.8.…”

Section: Preparation Of Leaf Homogenates and Intercellular Washing Flmentioning

confidence: 99%

Early defence responses of cowpea (Vigna sinensis L.) induced by non-pathogenic rust fungi

Fink

Haug

Deising

et al. 1991

Planta

View full text Add to dashboard Cite

Abstract. In cowpea (Vigna s&ensis L.) leaves the pathogenic rust fungus Uromyces vignae and the non-pathogens U. appendiculatus and U. viciae-fabae developed similarly to give rise to more than 80% haustorial mother cells. Whereas U. vignae was able to sporulate, the nonpathogens were stopped either after formation of some haustoria (U. appendiculatus) or immediately after formation of haustorial mother cells (U. viciae-fabae).Approximately 30% of the cells in contact with haustorial mother cells of the two non-pathogens showed autofluorescence and deposition of phloroglucinol/HC1-positive material. The early defence reactions of V. sinensis include induction of phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and extracellular peroxidase (POD, EC 1.11.1.7) activity as early as 10h and 24h after inoculation, respectively. Probing Western blots with heterologous monospecific anti-PAL serum showed that pathogenesis-induced increases in enzyme activity are the result of de novo synthesis. Native polyacrylamide gel electrophoresis revealed the specific induction of two extracellular acidic POD forms in cowpea inoculated with the non-pathogens. Both PAL and apoplasmic POD activities were highest in U. viciae-fabae-, intermediate in U. appendiculatus-and low in U. vignae-inoculated or talcum-treated control plants. The timing of increasing PAL and extracellular POD activities in relation to deposition of lignin or lignin-like material in mesophyll cell walls indicates the involvement of lignification in the early defence of V. sinensis against biotrophic fungal parasites. Analysis of the substrate specificity of the inducible POD forms, separated and partially purified by chromatofocusing, showed that apoplasmic PODs preferentially oxidize the naturally occurring substrate coniferyl alcohol. These results support the assumption that POD-mediated lignification is involved in the expression of non-host resistance of cowpea to rust fungi. * To whom correspondence should be addressed Abbreviations: IWF = intercellular washing fluid; PAL = phenylalanine ammonia-lyase; p.i. = post inoculation ; POD = peroxidase; SDS-PAGE = sodium dodecyl sulfate-polyacrylamide gel electrophoresis

show abstract

Phenylalanine Ammonia-lyase, Tyrosine Ammonia-lyase, and Lignin in Wheat Inoculated with Erysiphe graminis f. sp. tritici

Cited by 52 publications

References 0 publications

Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus

Patterns of post-infectional protein synthesis in barley carrying different genes for resistance to the powdery mildew fungus

Metabolite profiling of sheath blight disease resistance in rice: in the case of positive ion mode analysis by CE/TOF-MS

Early defence responses of cowpea (Vigna sinensis L.) induced by non-pathogenic rust fungi

Contact Info

Product

Resources

About