Phenyl alanine & Tyrosine Amino acids Coated Magnetic Nanoparticles: Preparation and Toxicity study

Abstract

In this study we reported the synthesis of L-phenyl alanine (Phe) & L-tyrosine (Tyr) Natural Amino acids coated iron oxide magnetic nanoparticles under one-pot and in situ reaction. Functionalized iron oxide magnetic nanoparticles were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Vibrating Sample Magnetometer (VSM), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) techniques. Cellular toxicity of amino acids coated iron oxide magnetic nanoparticles was also investigated on HEK-293 cell lines. Additionally, a hemolysis test of as prepared magnetic nanoparticles were performed. It was found that the synthesized Phe and Tyr coated magnetic nanoparticles (F@Phe NPs and F@Tyr NPs) were spherical in shape with an average size less than 25 nm, also the saturation magnetization (Ms) of the F@Phe NPs and F@Tyr NPs were about 30.02 and 58.23 emu/g, respectively, which was lower than those of bare FeO. The TGA results show that apart from this weight loss, the coated sample shows a weight loss of 5.48, and 6.88% respectively corresponding to loss of Tyr, and Phe which is coated on the FeO nanoparticles. At a high concentration, less than 2.92 and 3.13% hemolytic activity were observed for F@Phe NPs and F@Tyr NPs, respectively. The F@Phe NPs and F@Tyr NPs show the possibility of using this nanoparticles in the development of in vitro and in vivo pharmaceutical and biomedical fields due to do not possess a toxic effect, good ζ-potential and related small and narrow size distribution.

Poly(ethylene-imine)-Functionalized Magnetite Nanoparticles Derivatized with Folic Acid: Heating and Targeting Properties

“…The results obtained after 24 h (A) and 48 h (B) are very similar. This is in accordance with most studies in the literature, where the cytotoxicity tests are typically extended to 48 h at most [26,[61][62][63][64]. An evaluation of the interest of MNP-4 in MFHT was carried out at a concentration of 150 μg/mL on HEK (low FR overexpression), HeLa, and HepG2 cells (high FR overexpression) in a magnetic field of 12 kA/m and a frequency of 175 kHz, which, as mentioned, are reasonable parameters that are comparable to those used in the medical AMF devices (100 kHz and maximum of 15 kA/m, according to Magforce AG specifications (https://www.magforce.com/home/ Accessed on: 13 May 2021), and to those reported in nario changed when a magnetic field was applied, the effect on cell viability and colony formation being dependent on the expression of FR.…”

“…The results obtained after 24 h (A) and 48 h (B) are very similar. This is in accordance with most studies in the literature, where the cytotoxicity tests are typically extended to 48 h at most [26,[61][62][63][64].…”

Tuning the drug multimodal release through a co-assembly strategy based on magnetic gels

“…The use of phenylalanine has been reported by Nosrati et al 26 to improve the non-toxic effect compared to bare nanoparticles. Besides, Wu et al 27 have recently described the use of nanoparticles functionalized with l -phenylalanine to provide specific targeting to the overexpressed human large neutral amino acid transporter SLC7A5 (LAT-1) in cancer cells.…”

“…24,25 The particles were coupled with phenylalanine and diphenylalanine as functional groups after previous addition of (3-aminopropyl)triethoxysilane (APTES), not only to assess the role of aromatic rings and cationic amine group as a novel strategy in co-assembly, but also to improve the biocompatibility. 26,27 Various techniques were used to confirm the functionalization, including UV-Vis spectroscopy, DLS and Raman spectroscopy. Further, the particles iron content, as well as the structural and magnetic properties were also assessed and characterized through spectroscopic techniques, STEM, XRD and SQUID.…”

In vitro inhibition of tumor growth by low-dose iron oxide nanoparticles activating macrophages

“…12,13 Chrysin-loaded L-phenyl alanines (Phe)-coated IONPs with nontoxicity and biocompatibility are appropriate for chrysin delivery or other hydrophobic therapeutic agents. 14 IONPs coated with bovine serum albumin to form core–shell nanoparticles, are not only an appropriate candidate for magnetic resonance imaging (MRI) techniques, but also improve the possibility of applications in cellular labeling, drug and diagnostic delivery. 15 Octapod IONPs are synthesized as high-performance T2 contrast agents for magnetic resonance imaging.…”