Phenotyping using semi-automated BIOLOG and conventional PCR for identification of Bacillus isolated from biofilm of sink drainage pipes

El‐Liethy, Mohamed Azab; Hemdan, Bahaa A.; El‐Taweel, Gamila E.

doi:10.1016/j.chnaes.2018.01.011

Cited by 17 publications

(11 citation statements)

References 26 publications

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“…The microplate was incubated at 37 °C for 24h. The reading was carried out automatically by the computerized MicroStation™ system (Biolog Inc, USA) with the fingerprint data which was previously fed into the software (OmniLog® Data Collection) and used to identify the bacteria from their phenotypic patterns in the GEN III MicroPlate [35, 36, 37].…”

Section: Methodsmentioning

confidence: 99%

Metagenomics analysis of bacterial structure communities within natural biofilm

Hemdan

El‐Liethy

ElMahdy

et al. 2019

Heliyon

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The bacterial profiles of natural household biofilm have not been widely investigated. The majorities of these bacterial lineages are not cultivable. Thus, this study aims (i) to enumerate some potential bacterial lineages using culture based method within biofilm samples and confirmed using Biolog GEN III and polymerase chain reaction (PCR). (ii) To investigate the bacterial profiles of communities in two biofilm samples using next generation sequencing (NGS). Forty biofilm samples were cultured and colonies of each selected prevailing potential lineages (E. coli, Salmonella entrica, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria monocytogenes) were selected for confirmation. From obtained results, the counts of the tested bacterial lineages in kitchen biofilm samples were greater than those in bathroom samples. Precision of PCR was higher than Biolog GEN III to confirm the bacterial isolates. Using NGS analysis, the results revealed that a total of 110,554 operational taxonomic units (OTUs) were obtained for two biofilm samples, representing kitchen and bathroom biofilm samples. The numbers of phyla in the kitchen biofilm sample (35 OTUs) was higher than that in bathroom sample (18 OTUs). A total of 435 genera were observed in the bathroom biofilm sample compared to only 256 in the kitchen sample. Evidences have shown that the empirical gadgets for biofilm investigation are becoming convenient and affordable. Many distinct bacterial lineages observed in biofilm are one of the most significant issues that threaten human health and lead to disease outbreaks.

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Section: Methodsmentioning

confidence: 99%

Metagenomics analysis of bacterial structure communities within natural biofilm

Hemdan

El‐Liethy

ElMahdy

et al. 2019

Heliyon

Self Cite

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“…However, polymeric materials such as PVC and PE were preferred in drinking water networks due to their easier use concerning other iron‐based materials (Hemdan & Elliethy, 2016). Additionally, total heterotrophic counts (HPCs) and targeted media for microorganisms are still frequently used for regulating the growth of bacteria in drinking water, for instance by national government sanitation facilities inspectors and the industrial water sector, based on existing regulations (El‐Liethy, Hemdan, & El‐Taweel, 2018; Liu et al., 2017). In the present study, the growth rate and counts of E. coli biofilm, which was developed on six different pipe materials, was explored on two growth media including, plate count agar (PCA) and Rapid Hicoliform agar (RHA) every 15 days intervals.…”

Section: Resultsmentioning

confidence: 99%

The destruction of Escherichia coli adhered to pipe surfaces in a model drinking water distribution system via various antibiofilm agents

Hemdan

El‐Liethy

El‐Taweel

2020

Water Environment Research

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The aim of the study is to estimate the effectiveness of three antibiofilm agents against Escherichia coli biofilm that formed in six different types of pipelines. A laboratoryscale water system was built for this work to allow for the creation of biofilm in the pipelines studied. The level of the growth rate of E. coli biofilm cells was monitored over 90 days on those tested pipe materials. The results of bacterial cell densities displayed that the highest biofilm growth was observed in the biofilm formed on the iron (Fe) pipe. In contrast, the biofilm formation rate was significantly lower on copper (Cu) pipe compared to other materials. Three antibiofilm agents, including chlorine, silver ions (Ag +), and silver nanoparticles (AgNPs), were employed to eradicate the biofilm cells. E. coli counts indicated that AgNPs are more efficient in destructing any formed biofilm cells on all tested materials. At the same time, the chlorine was only useful in the case of biofilm developed on plastic and Cu. However, the antibiofilm efficiency of Ag + performs similarly to chlorine against E. coli biofilm cells. Ultimately, AgNPs are considred the most powerful antibiofilm agent among the other agents toward the biofilm cells in their maturation stage, which offers an encouraging way for the long-term functioning of water systems. © 2020 Water Environment Federation • Practitioner points • The growth rate of E. coli biofilm cells was investigated on different materials. • The count of biofilm cells developed on iron pipes was higher than other materials. • The E. coli biofilm on iron pipe could resist chlorine and AgNPs to a large extent. • The developed biofilm on copper pipe was more sensitive to chlorine, Ag +. and AgNPs. • The biofilm cells could be easily eradicated from plastic-based materials with all tested disinfectants.

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“…The inoculated MicroPlate incubated at 37 o C for the identification of bacterial strains. The identification procedures achieved by comparing with the Biolog's microbial identification systems software in a semi-automated way [15].…”

Section: Characterization Of the Bioactive Bacterial Strains (Biowish)mentioning

confidence: 99%

Assessment of biological augmentation technology of hazardous pollutants existing in drainage water in Bahr El-Baqar drain, Egypt

et al. 2019

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T HE objectives of the present study were to improve the quality of drainage water for reuse through the reduction of COD, BOD, TSS, TN, TP, and ammonia, also the removal of odor and detoxification of toxic compounds. The designed system for the treatment of drainage wastewater collected from Bahr El-Baqar drains was performed. The bioactive bacterial strains were applied in this system and followed by the electrocoagulation process. The phyicochemical and biological parameters were estimated for raw and treated drainage wastewater and compare the quality of treated drainage water according to law 1948. The results acquired pointed out the level of physicochemical items, and the numbers of bacterial indicators in Bahr El-Baqar drainage wastewater have been decreased after biological augmentation treatment after 6, 12 and 24 h. As well, The pH was around 7.2 and 7.4 after biological treatment (Table 3

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Phenotyping using semi-automated BIOLOG and conventional PCR for identification of Bacillus isolated from biofilm of sink drainage pipes

Cited by 17 publications

References 26 publications

Metagenomics analysis of bacterial structure communities within natural biofilm

Metagenomics analysis of bacterial structure communities within natural biofilm

The destruction of Escherichia coli adhered to pipe surfaces in a model drinking water distribution system via various antibiofilm agents

Assessment of biological augmentation technology of hazardous pollutants existing in drainage water in Bahr El-Baqar drain, Egypt

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