Phenotyping of sensory and sympathetic ganglion neurons of a galanin-overexpressing mouse—Possible implications for pain processing

Brumovsky, Pablo Rodolfo; Hygge-Blakeman, Karin; Villar, Marcelo J.; Watanabe, Masahiko; Wiesenfeld‐Hallin, Zsuzsanna; Hökfelt, Tomas

doi:10.1016/j.jchemneu.2006.02.001

Cited by 28 publications

(19 citation statements)

References 102 publications

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“…For the assessment of neuronal size, a grid (30 mm Â 30 mm) attached to the ocular of the microscope was used. Galanin-IR neuronal profiles similar in size to those observed in control ganglia (<30 mm in diameter) were grouped as small-sized, whereas neuronal profiles exhibiting diameters >30 mm were defined as medium to large-sized (Brumovsky et al, 2006).…”

Section: Quantificationmentioning

confidence: 99%

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Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve

Coronel

Brumovsky

Hökfelt

et al. 2008

Journal of Chemical Neuroanatomy

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Section: Quantificationmentioning

confidence: 99%

“…In fact, three galanin receptors have been cloned so far: GalR 1 , -R 2 and -R 3 (Branchek et al, 2000). In normal conditions, both GalR 1 and -R 2 have been detected in DRGs (see Kerekes et al, 2003) and spinal cord (see Waters and Krause, 2000;Brumovsky et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve

Coronel

Brumovsky

Hökfelt

et al. 2008

Journal of Chemical Neuroanatomy

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“…Gal overexpressed mice exhibited a strong Gal-like immunoreactivity in most SCG neuron profi les. The overexpression of the peptide in SCG neurons was paralleled by increased mRNA levels (Brumovsky et al, 2006). Gal is induced in autonomic neurons after peripheral nerve lesion.…”

Section: Introductionmentioning

confidence: 87%

Effects of Alpha 1- and Alpha 2-Adrenoreceptor Stimulation on Galanin mRNA Expression in Primary Cultured Superior Cervical Ganglion Neurons

Xing¹,

Chen²,

Liu³

et al. 2011

Biomolecules and Therapeutics

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Abstractan inhibitory modulator of cholinergic and noradrenergic neurotransmission (Miller et al., 1999;Van Hoomissen et al., 2004;Landry et al., 2005;Hobson et al., 2006). Gal is co-localized with norepinephrine (NE) in several regions of the brain, such as locus coeruleus, bed nucleus of the stria terminalis, amygdala, cortex and hippocampus (Hokfelt et al., 1998;Kozicz, 2001;Xu et al., 2001;Holmes et al., 2002;Barrera et al., 2006;Ogren et al., 2006;Simpson et al., 2006). Gal hyperpolarizes noradrenergic locus coeruleus neurons at high concentrations (10 -6 -10 -7 mol/L) and enhances NE-induced hyperpolarization at low concentrations (10 -9 mol/L) (Hokfelt et al., 1998). It has been shown that Gal expression was regulated by NE in the magnocellular neurons of supraoptic nucleus in an ex vivo acute model of rat hypothalamic slices (Melnikova et al., 2006) and in cultured dorsal root ganglion (DRG) neuronsOriginal Article

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“…It affects pain threshold and has developmental and trophic effects (Jimenez-Andrade et al 2005;Lu et al 2005;Brumovsky et al 2006;Shi et al 2006). Galanin is thought to play only a minor role in nociception under normal conditions.…”

Section: Introductionmentioning

confidence: 99%

“…There is evidence that upregulated galanin has trophic actions as well as influencing pain processing (Landry et al 2005). Galanin acts at three Gprotein-coupled receptors, galanin receptors (GalR1, GalR2, and GalR3; Shi et al 2006;Brumovsky et al 2006). It has been suggested that GalR1 and/or GalR3 activation induced analgesia at the level of spinal cord (Liu and Hokfelt 2002;Jimenez-Andrade et al 2004), while activation of GalR2 plays a pronociceptive role and enhances capsaicin-induced nociception in the periphery (Kerekes et al 2003;JimenezAndrade et al 2004JimenezAndrade et al , 2005JimenezAndrade et al , 2006.…”

Section: Introductionmentioning

confidence: 99%

Regulation of galanin and galanin receptor 2 expression by capsaicin in primary cultured dorsal root ganglion neurons

Liu

2008

In Vitro Cell.Dev.Biol.-Animal

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Galanin is a 29-amino-acid neuropeptide expressed in dorsal root ganglion (DRG) neurons which is thought to play a role in modulation of nociception in neuropathic states. Activation of galanin receptor 2 (GalR2) plays a pronociceptive role and enhances capsaicin-induced nociception in the periphery. GalR2 and vanilloid receptor 1 (VR1) are co-expressed in DRG neurons. Capsaicin evokes acute pain via activation of VR1 expressed in primary sensory neurons. It is not known to what extent galanin and its receptor GalR2 expression is regulated by capsaicin in DRG neurons. Effects of acute (4 h) or chronic (4 d) treatment with capsaicin at different concentrations (0.01, 0.1, 1 micromol/L) on galanin and GalR2 expression in primary cultured DRG neurons were investigated in the present study. Our results showed that acute exposure of high concentration capsaicin (1 micromol/L) increased galanin expression, whereas chronic exposure of low concentration capsaicin (0.01, 0.1 micromol/L) promoted galanin expression. Only chronic exposure of 0.1 micromol/L concentration capsaicin could elevate GalR2 expression, whereas capsaicin did not have this effect at any other conditions in this experiment. These results indicated that certain concentrations or exposure time of capsaicin stimulation may be relevant to upregulation of galanin and its receptor GalR2 expression in DRG cultures suggesting a response to peripheral neuronal stimulation. And also, capsaicin-induced GalR2 expression may be also modulated by capsaicin-induced galanin expression. The possible significance of the neurotransmission of nociceptive information involved in galanin or GalR2 expression caused by capsaicin is still to be clarified.

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Phenotyping of sensory and sympathetic ganglion neurons of a galanin-overexpressing mouse—Possible implications for pain processing

Cited by 28 publications

References 102 publications

Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve

Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve

Effects of Alpha 1- and Alpha 2-Adrenoreceptor Stimulation on Galanin mRNA Expression in Primary Cultured Superior Cervical Ganglion Neurons

Regulation of galanin and galanin receptor 2 expression by capsaicin in primary cultured dorsal root ganglion neurons

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