Phenotyping of Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole

Abstract

Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant growth regulator through the anti-gibberellin activity. And notably, the reduction of Brassica napus L. growth can be attributed to the inhibition of gibberellin biosynthesis at the stage of conversion of ent-kaurene to entkaurenoic acid. We describe here an in vitro experiment studying the relationship between epoxiconazole in culture medium (0 mg L⁻¹, 0.120 mg L⁻¹ and 0.200 mg L⁻¹) and the phenotyping (root and shoot growth) of three varieties of winter rapeseeds (Brassica napus L. var. Catalina, var. ES Astrid and var. Toccata). Plantlets fungicide content was quantified using the QuEChERS extraction method following by an automated UHPLC-MS/MS analysis. Results showed that the shoots and roots growth of Brassica napus L. plantlets was significantly inhibited by epoxiconazole at 0.120 mg L⁻¹ independently of the variety. The concentration of 0.200 mg L⁻¹ leaded to necrosis and anthocyanosis symptoms and can be considered as lethal for in vitro growing explants. The huge epoxiconazole absorption by rapeseed plantlets clearly showed a dose-dependent relationship and was closely similar for the three varieties.

Exogenous tryptophan application improves cadmium tolerance and inhibits cadmium upward transport in broccoli (Brassica oleracea var. italica)

“…IGS is a sulfur-containing secondary metabolite, whose biosynthesis requires GSH as a sulfur donor. It has been frequently reported that the biosynthesis of IGS was responsive to Cd stress ( Jakovljević et al, 2013 ; Durenne et al, 2017 ), although the physiological significance of this response is not clear. Our study found that Trp inhibited Cd-induced IGS biosynthesis.…”

A Systematic Review on Effects of Nitrogen Fertilizer Levels on Cabbage (Brassica oleracea var. capitata L.) Production in Ethiopia

“…Organic manure and biofertilizers improve soil health and crop quality, as evidenced by a study on red cabbage cultivation assessing nutrient efficiency, productivity, and soil sustainability [ 15 ]. In addition, a field experiment was conducted to evaluate the growth, productivity, and economics of cabbage in the grid zone as influenced by different levels of zinc and sulfur reported that growth parameters such as plant height, plant spread, number of leaves per plant at all crop growth stages, yield attributing characters, length and diameter of head and weight per head, cabbage yield per hectare net return, and benefit cost ratio increased with increasing levels of sulfur from 20 to 80 kg/ha and zinc from 2 to 8 kg/ha and the crop responded only up to 60 kg S/ ha and 6 kg Zn/ha [ 61 , 62 ]. During a 2017 experiment in Ghana's Kintampo North Municipality, evaluating cabbage response to various soil amendments from August to November, poultry manure matched NPK (15-15-15) and outperformed other treatments across all measured parameters, while cow dung and goat manure also exhibited promising results [ 63 , 64 ].…”

PENGARUH BEBERAPA JENIS DAN DOSIS FUNGISIDA TERHADAP PENYAKIT HAWAR DAUN (Helminthosporium sp.) DAN KEANEKARAGAMAN JAMUR ENDOFIT PADA DAUN TANAMAN JAGUNG (Zea mays L.)

“…Epoksikonazol adalah fungisida triazole yang berspektrum luas. Epoxiconazole mampu mengganggu sintesis membran sel jamur sebagai penghambat biosintesis ergosterol serta Bahan aktif pyraclostrobin mampu mengurangi infeksi endofit dibandingkan dengan kontrol (Durenne et al, 2018). Bahan aktif difenokonazol mampu menurunkan frekuensi Fusarium.…”