Phenotyping of E. coli serotypes associated to oedema disease

Mattsson, Sigbrit; Wallgren, Per

doi:10.1186/1751-0147-50-13

Cited by 6 publications

(5 citation statements)

References 22 publications

(26 reference statements)

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“…This species also includes different virulence factors and serotypes associated with intestinal and extraintestinal diseases. The antigen O and H (O polysaccharide and flagellin, respectively) are the two major antigens of Gram-negative bacteria (Blanco et al 2003, Hussein 2007, Mattsson & Wallgren 2008. Since the early 1940s, the gold-standard technique for O and H characterization has been the agglutination test for E. coli serotyping, with 187 "O" and 53 "H" being characterized to date.…”

Section: Introductionmentioning

confidence: 99%

“…Since the early 1940s, the gold-standard technique for O and H characterization has been the agglutination test for E. coli serotyping, with 187 "O" and 53 "H" being characterized to date. Serology has been used to track strains in epide-miological studies and has allowed the characterization of pathogenic E. coli serotypes (Mattsson & Wallgren 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Several serotypes are associated with human illnesses and all of them are pathotypes associated with animals: O2: H5, 6, 7, 29; O8:H2, 19, 21; O20:H19; O22:H8; O25:H2; O26:H11, HNT; O45:H2; O91:H10, 21; O103:H2; O105:H18; O111:H8; O112ac:H19, 5; O113:H21; O118:H16; O119:H2, 6; O121:H19; O128:H2; O128ab:H2, 6; O145:H25, 28, O146:H21; O153:H25; O157:H7; O163:H19; O165:H25; O174:H2; 721; ONT:H2, 8, 11, 25, 28, 33, and 41 (Blanco et al 2003, Hussein 2007, Mattsson & Wallgren 2008. However, several difficulties have been observed in H antigen serotyping: (I) the expression of H-antigens can be dependent on various environmental signals; (II) the identification of H antigen is a time-consuming process and requires the use of 53 specific antisera; and (III) there are a high number of cross-reactions among E. coli strains (Blanco et al 2003, Hussein 2007, Mattsson & Wallgren 2008).…”

Section: Introductionmentioning

confidence: 99%

“…However, several difficulties have been observed in H antigen serotyping: (I) the expression of H-antigens can be dependent on various environmental signals; (II) the identification of H antigen is a time-consuming process and requires the use of 53 specific antisera; and (III) there are a high number of cross-reactions among E. coli strains (Blanco et al 2003, Hussein 2007, Mattsson & Wallgren 2008). This procedure is important, because identification of a particular H antigen saves time and reduces the number of antisera required to identify the O antigens in E. coli strains (Blanco et al 1992, Moreno et al 2006.…”

Section: Introductionmentioning

confidence: 99%

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Identification of new flagellin-encoding fliC genes in Escherichia coli isolated from domestic animals using RFLP-PCR and sequencing methods

et al. 2013

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Identification of Escherichia coli requires knowledge regarding the prevalent serotypes and virulence factors profiles allows the classification in pathogenic/non-pathogenic. However, some of these bacteria do not express flagellar antigen in vitro. In this case the PCR--restriction fragment length polymorphism (RFLP-PCR) and sequencing of the fliC may be suitable for the identification of antigens by replacing the traditional serology. We studied 17 samples of E. coli isolated from animals and presenting antigen H nontypeable (HNT). The H antigens were characterized by PCR-RFLP and sequencing of fliC gene. Three new flagellin genes were identified, for which specific antisera were obtained. The PCR-RFLP was shown to be faster than the serotyping H antigen in E. coli, provided information on some characteristics of these antigens and indicated the presence of new genes fliC.INDEX TERMS: Escherichia coli, H antigen, PCR-RFLP, sequencing, serotyping. Os antígenos H foram caracterizados por PCR-RFLP e sequenciamento do gene fliC. Três novos genes da flagelina foram identificados, para os quais anti-soros específicos foram obtidos. A técnica PCR-RFLP mostrou-se mais rápida que a sorotipagem do antígeno H em E. coli, fornecendo informações sobre algumas características desses antígenos e indicou a presença de novos genes fliC.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of new flagellin-encoding fliC genes in Escherichia coli isolated from domestic animals using RFLP-PCR and sequencing methods

et al. 2013

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“…ONT:H2,H8,H11,H25,H28,H33,H41, incluindo bovinos, suínos e caprinos (Blanco et al, 2003;Hussein, 2007;Mattsson & Wallgren, 2008). Considerando estas associações, a determinação do antígeno O pode ser guiada pela identificação dos antígenos H entre amostras patogênicas.…”

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