Phenotyping of clinically significant blood group antigens among the South Indian donor population

Subramaniyan, Rajeswari

doi:10.1016/j.htct.2021.11.012

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…The R1R1 phenotype was the most frequent among Rh(D) positive donors and Rr among the Rh(D) negative donors. This is similar to other studies from India [9][10][11][12][13][14].…”

Section: Resultssupporting

confidence: 92%

“…Detail Kell, Duffy and Kidd phenotype frequencies among the donors is illustrated in Table-5. In the present study, the ABO blood group antigen frequencies showed the prevalence as O > B > A > AB which was similar to other studies from South India [8,9] but in contrast to some Indian studies where B blood group was reported more prevalent [10,11]. It is due to the multiethnic population of our country and as a result studies in different region of India reported varied prevalence of ABO blood group.…”

Section: Resultssupporting

confidence: 89%

“…It is due to the multiethnic population of our country and as a result studies in different region of India reported varied prevalence of ABO blood group. In India, the frequency of D negative antigen varies from 5% to 10% [8][9][10][11][12]. The frequency of Rh(D) positive in our study was 92%, whereas only 8% were Rh(D) negative.…”

Section: Resultsmentioning

confidence: 49%

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Clinically significant minor blood group antigens amongst South Indian donor population

John,

Achankunju,

Suma

et al. 2023

IMC Journal of Medical Science

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Background and objectives: Distribution of blood group antigen varies among different races. It is important to know the distribution of these antigens so as to provide a donor database that aid in providing compatible blood units for patients with multiple alloantibodies. The present study was conducted to determine the distribution of clinically significant minor blood group antigens amongst the South Indian blood donors. Materials and methods: Blood samples were collected from healthy regular repeat voluntary blood donors of same ethnicity attending a tertiary care hospital in South Kerala. Clinically significant blood antigens of the ABO, Rh (D, C, c, E, and e), Kell, Duffy and Kidd blood group systems were determined. The ABO and Rh(D) grouping were performed by tube technique using monoclonal antisera. Column agglutination technique was used to phenotype Rh, Kell, Duffy and Kidd antigens. Results: Total 200 healthy repeat voluntary blood donors were enrolled in the study. Out of 200 donors, 92% were RhD positive. Among the Rh antigens, the e antigen was positive in 97.8 % and 100% among the Rh(D) positive and Rh(D) negative donors respectively. No E antigen was detected in RhD negative donors. Total 6 and 2 Rh phenotypes were observed among the Rh(D) positive and negative donors respectively. R1R1 and Rr were the most frequent phenotypes among the RhD positive and negative donors (47.28% and 93.75%) respectively. Among the Kell blood group antigens, K and Kpb antigens were present in 100% of our donors while in Duffy and Kidd system Fya and Jka were most predominant (89% and 87%) respectively. Conclusions: The findings of the present study would be helpful in developing in-house panel cells. Moreover, a rare donor registry of donors typed negative for a high-frequency antigen can be formulated. IMC J Med Sci. 2024; 18(1):004. DOI: https://doi.org/10.55010/imcjms.18.004 *Correspondence: Soonam John, Department of Transfusion Medicine, Government Medical College, Parippally, Kollam, Kerala,India. Email: johnsoonam@gmail.com

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“…The R1R1 phenotype was the most frequent among Rh(D) positive donors and Rr among the Rh(D) negative donors. This is similar to other studies from India [9][10][11][12][13][14].…”

Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 89%

See 1 more Smart Citation

Clinically significant minor blood group antigens amongst South Indian donor population

John,

Achankunju,

Suma

et al. 2023

IMC Journal of Medical Science

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Section: Discussionsupporting

confidence: 93%

“…Lastly, the null Jk(a-b-) phenotype was not detected in Ethiopian blood donors. These finding was consistent with studies conducted in the United Arab Emirates, North and South Indian donor population (17,19,20) It was challenging to compare the results of different studies because of the heterogeneity of the populations' involved, varied screening protocols, and differences in the techniques used for antigen and antibody identification. Besides, the distribution of the blood group antigens among different racial-ethnic groups shows different findings.…”

Section: Acknowledgmentssupporting

confidence: 86%

Assessment of Minor Blood Group System Antigens and Their Phenotype among Voluntary Blood Donors in Ethiopian Blood and Tissue Bank Service, Addis Ababa, Ethiopia

Jenbere,

Urgessa,

Tibebu

2023

Ethiop J Health Sci

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Background: Red blood cell antigens are numerous in structural and functional diversity; some are proteins while others are carbohydrates. The international society of blood transfusion currently recognized 43 blood group systems containing 349 red cell antigens. It also acknowledged 9 blood group systems (ABO, Rhesus, Kell, Duffy, Kidd, MNS, P, Lewis, and Lutheran) that are clinically significant and associated with hemolytic transfusion reactions as well as hemolytic disease of fetuses and newborns. The objective of this study was to assess the distribution of minor blood group antigens and their phenotype among voluntary blood donors in Ethiopian blood and tissue bank service in Addis Ababa. Method: A cross-sectional study was conducted from January to March 2022 among 260 volunteer blood donors to determine minor blood group antigens and their phenotype at EBTBS, Addis Ababa, Ethiopia. Tests were performed using Galileo Neo Immucor, which is fully automated Immunohematology analyzer. Result: A total of 260 blood donors were screened of which 153 (59%) were males. The antigen frequencies of minor blood group systems were: Fy(a), Fy(b), Jk(a), Jk(b), k, S, s were 33.5%, 43.5%, 97.7%, 40.4%, 100%, 45%, 90%, respectively. Regarding phenotype distribution, the most common phenotypes were: Duffy Fy (a-b+) 36.9%, MNS S-s+ 55% and Kidd Jk (a+b-) 59.6%. Conclusion: This study highlights the frequencies of Fy(a), Fy(b), Jk(a), Jk(b), k, S and s blood group antigens and their phenotypes in volunteer blood donors at EBTBS, Addis Ababa. For the management of alloimmunization cases in transfused patients, knowledge of these minor blood group antigens is relevant.

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Homologous blood transfusion and doping: Where are we now?

Marchand,

Ericsson

2024

Drug Testing and Analysis

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Homologous blood transfusion (HBT) is used for doping in endurance sports since the 1960s. The blood comes from a compatible donor, that is, someone with a compatible ABO and rhesus blood group. Despite been prohibited by the IOC in 1985, no detection method was available until 2003. Then came the idea to use red blood cells (RBC) minor blood groups antigens that constitute an “identity” card of someone's RBC to detect the presence of a second RBC population. The method validated for doping control samples uses flow cytometry after incubation of isolated RBC with eight to 12 primary antibodies against specific minor blood groups antigens. The presence of double populations of RBC is revealed by a major and a minor peak in a fluorescence histogram. The sensitivity was estimated sufficient to detect HBT for a few weeks. Despite the complexity and cost of the method, right after its application in 2004, several cases of HBT were identified but the number of cases dropped rapidly over the years. In the 2010s, other ways to detect HBT were developed and evaluated: indirect detection using the Athlete Biological Passport approach, and a few years later forensic DNA analysis to establish the presence of two different DNA in a blood sample after HBT. Despite the high specificity of the latter, the sensitivity was recently questioned in vivo. Nowadays, the flow cytometry method remains the method of choice for HBT detection and recent investigations helped to simplify the method and increase its specificity and sensitivity.

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Phenotyping of clinically significant blood group antigens among the South Indian donor population

Cited by 4 publications

References 14 publications

Clinically significant minor blood group antigens amongst South Indian donor population

Clinically significant minor blood group antigens amongst South Indian donor population

Assessment of Minor Blood Group System Antigens and Their Phenotype among Voluntary Blood Donors in Ethiopian Blood and Tissue Bank Service, Addis Ababa, Ethiopia

Homologous blood transfusion and doping: Where are we now?

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