Phenotypical and Functional Characterization of Double‐Negative (CD4<sup>‐</sup>CD8<sup>‐</sup>) αβ T‐Cell Receptor Positive Cells from an Immunodeficient Patient

Illum, Niels Ove; Ralfkiær, Elisabeth; Pallesen, Gorm; Geisler, Carsten

doi:10.1111/j.1365-3083.1991.tb01587.x

Cited by 12 publications

(6 citation statements)

References 43 publications

(17 reference statements)

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“…Note added in proof Since the submission ofthe manuscript for this article, Illum et al (35) have reported a patient with lymphadenopathy, splenomegaly, and humoral immunodeficiency, who exhibited an expansion of TCRa/13 CD4-CD8-T cells. The phenotypic and functional properties of the double negative T cells from this patient were similar to those of our two patients.…”

Section: Discussionmentioning

confidence: 99%

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease.

Sneller¹,

Straus²,

Jaffe³

et al. 1992

J. Clin. Invest.

284

175

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In mice, the two distinct autosomal recessive genes lpr and gid can induce a syndrome characterized by autoantibody formation and the progressive accumulation of an unusual CD4-CD8-T cell population in peripheral lymphoid tissue. This phenotype does not precisely mirror any human disease. In this report we describe two patients with a progressive lymphoproliferative disorder associated with autoimmunity. The peripheral blood and lymph nodes of these patients contained large numbers of an unusual CD4 -CD8 -T cell population. These CD4 -CD8 -T cells express surface markers characteristic of mature peripheral blood T cells (CD3, CD2, CD5), express the a /If form of the T cell receptor, and do not express surface markers characteristic of immature thymocytes (CD1) or NK cells (CD16, CD56). Functionally, these cells exhibited deficient proliferation and lymphokine production upon stimulation with mitogenic antibodies to CD3 or CD2. Both proliferation and lymphokine production could be augmented by co-stimulation with an antibody directed at the CD28 determinant. The clinical and immunological features of this syndrome resemble the lymphoproliferative/autoimmune disease seen in lpr and gid mice. (J. Clin. Invest. 1992.90:334-341.)

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Section: Discussionmentioning

confidence: 99%

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease.

Sneller¹,

Straus²,

Jaffe³

et al. 1992

J. Clin. Invest.

284

175

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“…Studies in human identified a population of DN T cells in a patient with immunodeficiency, lymphocytosis, lymphadenopathy, and hepatosplenomegaly. These cells showed an impaired proliferative response upon stimulation with mitogens or anti-CD3 plus anti-CD28 but were not tested for their immunosuppressive activity [67].…”

Section: Dn T Cells As the Saviours Of The Immune Systemmentioning

confidence: 99%

CD3+CD4−CD8− (double negative) T cells: Saviours or villains of the immune response?

D’Acquisto¹,

Crompton²

2011

Biochemical Pharmacology

155

132

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To cite this version:Fulvio D'Acquisto, Tessa Crompton. CD3CD4CD8 (double negative) T cells: saviours or villains of the immune response?. Biochemical Pharmacology, Elsevier, 2011, 82 (4) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 29A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64

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“…They represent a small subpopulation of peripheral blood lymphocytes (PBL) in most healthy adults but are increased in some patients with systemic and cutaneous autoimmune disorders (17)(18)(19), in children suffering from certain genetic immunodeficiency syndromes (20,21), in some individuals infected with T-cell leukemia virus type I or human immunodeficiency virus (22,23), and in some apparently healthy individuals (24) (37). mAbs were anti-Leu-4 (CD3), anti-Leu-3 (CD4), anti-Leu-2a (CD8), anti-Leu-15 (CD11b), anti-Leu-llc (CD16), antiinterleukin-2 receptor (CD25, p55), anti-Leu-19 (CD56), and antiLeu-7 (CD57), anti-TCR-1 (TCR a/(3), anti-TCRy/8-1 (TCRCy), anti-Leu-8 (L-selectin), and anti-HLA-DR, all purchased from Becton Dickinson, and 2H4 (CD45RA) from Coulter (Raritan, NJ) and UCHL1 (CD45RO) from Dako.…”

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confidence: 99%

Human T-cell receptor (TCR) alpha/beta + CD4-CD8- T cells express oligoclonal TCRs, share junctional motifs across TCR V beta-gene families, and phenotypically resemble memory T cells.

Brooks

Balk

Aupeix

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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Most human T cells express the TCR a/Il and either CD4 or CD8 molecules (single positive, SP); however, small numbers lack CD4 and CD8. In inbred mice, a/,B CD4-CD8-(double negative, DN) T cells preferentially express certain 13 variable region (Vp) marrow a//3 T cells also lack CD4 and CD8 and express a skewed TCR repertoire with preferential usage of Vp2 and limited junctional diversity (12). These findings have suggested that a/fl DN T cells may be subject to thymic and/or extrathymic selection pressures distinct from those generating a/fl SP T cells. Therefore, the physiologic potential of a/fl DN T cells as well as the ligands they recognize may also be unique. This has been supported by the isolation of human a/fl DN T cell clones, which lyse target cells through recognition ofthe nonpolymorphic MHC-like CD1 molecules (13).In humans, a/fl DN T cells have been identified in peripheral blood, thymus, and skin (14-16). They represent a small subpopulation of peripheral blood lymphocytes (PBL) in most healthy adults but are increased in some patients with systemic and cutaneous autoimmune disorders (17)(18)(19)

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Phenotypical and Functional Characterization of Double‐Negative (CD4^‐CD8^‐) αβ T‐Cell Receptor Positive Cells from an Immunodeficient Patient

Cited by 12 publications

References 43 publications

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease.

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease.

CD3+CD4−CD8− (double negative) T cells: Saviours or villains of the immune response?

Human T-cell receptor (TCR) alpha/beta + CD4-CD8- T cells express oligoclonal TCRs, share junctional motifs across TCR V beta-gene families, and phenotypically resemble memory T cells.

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Phenotypical and Functional Characterization of Double‐Negative (CD4‐CD8‐) αβ T‐Cell Receptor Positive Cells from an Immunodeficient Patient

Cited by 12 publications

References 43 publications

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease.

A novel lymphoproliferative/autoimmune syndrome resembling murine lpr/gld disease.

CD3+CD4−CD8− (double negative) T cells: Saviours or villains of the immune response?

Human T-cell receptor (TCR) alpha/beta + CD4-CD8- T cells express oligoclonal TCRs, share junctional motifs across TCR V beta-gene families, and phenotypically resemble memory T cells.

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Phenotypical and Functional Characterization of Double‐Negative (CD4^‐CD8^‐) αβ T‐Cell Receptor Positive Cells from an Immunodeficient Patient