Phenotypic variation of sweet sorghum after carbon ion beam irradiation

Brief Overview of Sweet Sorghum Irradiated by Carbon Ion Beam

“…Analysis of the PCR amplification and sequence alignment of the Hd1 and Hd3a genes revealed that there was no difference between KFJT-CK and KFTJ-1 in the case of Hd1 gene. In contrast, an insert fragment GA was found in KFTJ-1 and the corresponding fragment was not found in KFJT-CK for the Hd3a gene (Dong et al, 2015). Generally, the majority of the induced mutations were deletions in the mutants due to carbon ion irradiation (Hirano et al, 2012).…”

“…After carbon ion irradiation, some abnormal mutants were firstly isolated at the seedling stage, such as growing point vanishing, tip curling, plant withering and etiolation ( Figure 1, Dong et al, 2015). In addition, higher mutation frequencies of 9.1%, 8.1% and 8.5%, were found for stalk thickening, sugar content and plant withering, respectively.…”

“…However, the current varieties of sweet sorghum cannot meet the rapid growth demand in China for bio-ethanol production owing to its low sugar content. To breed novel varieties to provide excellent raw materials for bio-ethanol production from sweet sorghum, more work has been done for mutation breeding with carbon ion beam irradiation in sweet sorghum since 2006 (Dong et al, 2007;Dong et al, 2015). In this paper, we briefly summarized the biological effects induced by carbon ions, characters of early-maturity mutant, and revealed corresponding mechanism from the point of view of morphological, physiological and molecular levels.…”

“…To further validated the mutagenic effects of the carbon ions, correlation analysis was carried out between the irradiation dose and the main agronomic traits according to indexes which were measured at the mature stage, including node number, plant height, stalk diameter, sugar content and single stem weight (Dong et al, 2009;Dong et al, 2015). The analysis demonstrated that the irradiation dose was negatively correlated with both the node number and the sugar content.…”

Plant Mutation Breeding with Heavy Ion Irradiation at IMP

“…Triad, unequal separation of chromosome and asychronization of chromosomes segregation were found in KFJT-1 meiosis, but the most of chromosomal aberrations induced by carbon ions were repaired, the aberration rate of KFJT-1 being only 4.5%. Dong et al, 2008 In the case of phenotypic effects induced by heavy ion beam irradiation, Saintpaulia ionahta (Zhou et al, 2006), sweet sorghum Dong et al, 2015) and Arabidopsis thaliana were studied at IMP, respectively. For Saintpaulia ionahta, in vitro leaf explants samples were irradiated with carbon ion beams with linear energy transfer (LET) values in the range of 31-151 keV/um at different doses.…”