Phenotypic Variation ofHelicobacter pyloriIsolates from Geographically Distinct Regions Detected by Lectin Typing

Abstract

A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cristigali, and Triticum vulgaris were used to type the strains. The lectins were chosen for their specificities for sugars commonly encountered in the lipopolysaccharide of H. pylori. The isolates were received from their parent institutions as pellets of biomass and were typed at one of three centers (in Ireland, Sweden, and Estonia). All 16 possible lectin reaction patterns were observed in the study, with the isolates with the predominant pattern exhibiting reactions with all the lectins in the panel. For European patients suffering from gastritis, an association was noted between lectin reaction pattern MH4 and atrophic chronic gastritis; isolates with lectin reaction pattern MH4 were isolated from patients with atrophic chronic gastritis, whereas isolates with this pattern were not isolated from patients with chronic gastritis (P ‫؍‬ 0.0006). In addition, statistically significant relationships were noted between the lectin reaction pattern and the associated pathology of isolates from the Swedish population. Isolates with patterns MH13 and MH16, which had low lectin reactivities, correlated with nonulcer disease (P ‫؍‬ 0.0025 and P ‫؍‬ 0.0002, respectively), and all four isolates from adenocarcinoma patients were characterized as possessing reaction pattern MH16. In contrast, isolates with lectin reaction patterns MH1 and MH10, which had high lectin reactivities, were associated with ulcer disease (P ‫؍‬ 0.046 and P ‫؍‬ 0.0022, respectively).

A comparison of glycan expression and adhesion of mouse-adapted strains and clinical isolates ofHelicobacter pylori

“…Consistent with previous studies by others (Hynes et al , 1999, 2002), the lipopolysaccharide lectin‐typing patterns in our clinical isolates were highly variable. This was also the case for the mouse‐adapted strains, with no lectin‐typing pattern being associated with the ability of H. pylori strains to infect mice.…”

“…Preparation of bacterial lipopolysaccharides was as described previously by others (Hynes et al, 2002). Briefly, H. pylori samples were resuspended in 5 mL sterile PBS and disrupted by sonication on ice (Branson Sonifier Cell Disruptor) by 30-s pulses 10 times, with 1 min between each pulse.…”

“…The bacterial lipopolysaccharide lectin-mediated agglutination assay was modified from a lectin-typing method used previously by others (Hynes et al, 2002). Ulex europaeus (UEA-I), Lotus tetragonolobus (LTA), Concanavalin A (ConA), Glycine max (SBA) and Triticum vulgaris (WGA) were obtained from Sigma.…”

A mannan binding lectin is involved in cell–cell attachment in a toxic strain of Microcystis aeruginosa

“…The ability of carbohydrate binding proteins to interact preferentially with specific cell types has also led to attempts to use them as drug carrier (Sharma et al ., 2004) and for blood typing (Khan et al ., 2002). Important further applications include the diagnostic use of lectins for the identification of microorganisms (Hynes et al ., 2002) and the use of lectins for the visualization of extracellular matrices to determine the carbohydrate composition of, e.g. biofilms (Neu et al ., 2001).…”

Analysis of geospecific markers for Helicobacter pylori variants in patients from Japan and Nigeria by triple-locus nucleotide sequence typing

“…Isolates of H. pylori, irrespective of geographical location, have identical phenotypes as defined by conventional bacteriological tests (Owen et al, 2001b) although there is evidence that strains can differ in some expressed features such as vacuolating cytotoxin activity (Forsyth et al, 1998), lectin reaction patterns (Hynes et al, 2002) and interleukin-8 induction in gastric epithelial cells (Owen et al, 2003). In contrast, strains of H. pylori from unrelated individuals exhibit an unusually high level of genomic diversity as indicated by various fingerprinting techniques (Owen et al, 2001b).…”