Phenotypic variation and lability of the subunit composition of the hemocyanin of Uca pugilator

Callicott, Kenneth A.; Mangum, Charlotte P.

doi:10.1016/0022-0981(93)90102-t

Cited by 13 publications

(13 citation statements)

References 15 publications

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“…We have attempted to accurately represent the species investigated by choosing high-frequency phenotypes for those known to be polymorphic (Mangum, 1990(Mangum, , 1993aCallicott and Mangum, 1993; Mangum and Greaves, unpub. data; C. P. Mangum and K. T. White, unpub.…”

Section: Hc Subunit Compositionmentioning

confidence: 99%

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Subunit Composition and O2 Binding of the Crustacean Hemocyanins: Interspecific Relationships

Reese

Mangum

1994

The Biological Bulletin

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The monomeric subunit composition and oxygen binding properties of hemocyanins were examined in 9 taxonomic groups of 43 crustacean species and 1 hybrid. In native polyacrylamide electrophoresis the banding pattern was highly species specific, even in closely related congeners. In less closely related taxa, there was little apparent relationship between phylogenetic affinity and banding pattern. Within a taxonomic group, pH dependence was the most highly conserved and O2 affinity was the most diverse of the O2 binding properties investigated. In congeneric but not sibling species, O2 affinity was more highly correlated with an environmental variable such as temperature than with phylogenetic affinity. Only in very closely related groups found in similar environments were different O2 binding properties correlated with differences in particular electrophoretic bands.

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Section: Hc Subunit Compositionmentioning

confidence: 99%

“…Although the polypeptide composition of both artificial and natural 1 X 6-mers can be homogeneous, the native blood in a species contains more than one, and up to a dozen or more different, monomeric chains (reviewed by Markl, 1986, andMarkl andDecker, 1992; see also Callicott and Mangum, 1993;Mangum and Greaves, unpub. data).…”

Section: Introductionmentioning

confidence: 98%

Subunit Composition and O2 Binding of the Crustacean Hemocyanins: Interspecific Relationships

Reese

Mangum

1994

The Biological Bulletin

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“…However, a clear relation between phenotypes and environmental regimes has not yet been established, and physico/chemical factors like temperature and salinity do not totally account for the subunit composition variability. The results on U. pugilator reveal more pronounced differences at the edge of the geographic distribution, underlyining an environmental effect in a latitudinal gradient as a selective force for phenotypic differentiation in disjunct populations (Callicott and Mangum, 1993).…”

Section: Intra-specific Polymorphismmentioning

confidence: 82%

“…The clearest cases of within-species variation of Hc subunit composition are represented by the decapods Ocypodidae (Mangum, 1993b(Mangum, , 1996Callicott and Mangum, 1993;Mangum and Greaves, 1996) and amphipods (Hodgson and Spicer, 2001;Spicer and Hodgson, 2003a). In the genus Uca up to 17 polypeptide chains and 39 different subunit compositions in the oligomers have been detected among individuals of the same species, providing strong example of the extent of intra-specific phenotype heterogeneity (Mangum and Greaves, 1996).…”

Section: Intra-specific Polymorphismmentioning

confidence: 99%

The molecular heterogeneity of hemocyanin: Its role in the adaptive plasticity of Crustacea

Giomi

Beltramini

2007

Gene

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“…Numerous studies have been addressed at the intraspecific variability to analyze the correlation among functional and structural properties of Hc with the ontogenetic and ecological traits of animals (Sullivan et al 1974;Mangum and Rainer 1988;Callicott and Mangum 1993;Mangum and Greaves 1996;Durstewitz and Terwilliger 1997;Brown and Terwilliger 1998;Terwilliger and Dumler 2001). These studies have considered the molecular heterogeneity of Hc among populations as an essential feature for the adaptive processes suggesting that variations in the native subunit patterns generate complex hetero-hexamers and contribute to distinct oxygen-binding features (cooperativity, oxygen affinity, allosteric regulation, etc.).…”

Section: Introductionmentioning

confidence: 99%

Structural and functional heterogeneity of hemocyanin: intra- and inter-specific comparison in four species of portunid crabs (Crustacea: Portunidae)

et al. 2007

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This work is focused on hemocyanin (Hc) heterogeneity at population level and on the biochemical characterization of the different subunit patterns. Two different approaches have been used: we have characterized Hc subunit composition to describe the subunit polymorphism. Then, we have measured the Hc oxygen-binding affinity to disclose the physiological implications of such heterogeneity. In order to evaluate the intra-and inter-specific variability, different populations of Liocarcinus depurator, Liocarcinus marmoreus, Liocarcinus holsatus, Necora puber (Crustacea: Portunidae), from British Isles and Adriatic Sea, have been sampled. Results indicate that Hc polymorphism normally occurs at both intra-and interspecific levels and involves the protein subunit type.These evidences extend to the portunid Hc the concept of molecular heterogeneity within species that have been previously reported for other decapod and amphipod crustaceans. Besides, the results support the view that subunit composition of crustacean Hcs is almost species-specific, but also that closely related species share a common pattern. Furthermore, this heterogeneity corresponds to different stability of the native oligomers quaternary structure and different oxygen affinity. The results are discussed in relation with the environmental regimes that characterize the different sampling areas. In addition to Hc, the hemolymph collected in specimens from different sampling areas contained also a non-respiratory pseudo-Hc. This paper reports for the first time the occurrence of pseudo-Hc at the level of population.

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Phenotypic variation and lability of the subunit composition of the hemocyanin of Uca pugilator

Cited by 13 publications

References 15 publications

Subunit Composition and O2 Binding of the Crustacean Hemocyanins: Interspecific Relationships

Subunit Composition and O2 Binding of the Crustacean Hemocyanins: Interspecific Relationships

The molecular heterogeneity of hemocyanin: Its role in the adaptive plasticity of Crustacea

Structural and functional heterogeneity of hemocyanin: intra- and inter-specific comparison in four species of portunid crabs (Crustacea: Portunidae)

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