Pinus wallichiana, commonly known as the Himalayan blue pine, holds significant ecological and economic importance in the northwestern Himalayan region. Understanding the genetic traits and variability within its seedling population is essential for sustainable forest management and conservation efforts. This study aimed to quantify and assess the genetic traits of Pinus wallichiana seedlings within a nursery environment situated in the northwestern Himalayas. Our research involved the collection and analysis of data from a representative sample of Pinus wallichiana seedlings from different sites in Himachal Pradesh in 2019–2020. Results revealed a diverse genetic pool with notable heritability for key traits, highlighting the potential for selective breeding and genetic improvement programs. Furthermore, our findings provide valuable insights into the adaptation and resilience of Pinus wallichiana to changing environmental conditions, which is crucial for addressing the challenges posed by climate change. The quantification of genetic traits in this study not only enhances our understanding of the species but also offers practical applications for forest managers and policymakers in the region. This research contributes to the broader context of forest genetics and underscores the importance of genetic conservation efforts for the sustainable management of Pinus wallichiana in the northwestern Himalayas.