Phenotypic Variability of Lipids of Escherichia Coli Grown in Chemostat Culture

Calcott, Peter H.; Petty, Rita S.

doi:10.1111/j.1574-6941.1980.tb01569.x

Cited by 6 publications

(7 citation statements)

References 12 publications

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“…In contrast, little or no change in lipid content of carbon-limited cultures was found for E. coli (Damoglou & Dawes, 1968;Calcott & Petty, 1980) or P . aeruginosa (Gilbert & Brown, 1978).…”

Section: Effects Of Growth Ratementioning

confidence: 73%

“…Also, the DPG:PG ratio rose markedly with increasing growth rate for E. coli (Calcott & Petty, 1980), and slightly for P. aeruginosa (Gilbert & Brown, 1978), but fell for B. cepacia. The accumulation of DPG by Gramnegative bacteria is often observed as the growth rate declines and cells enter the stationary phase in batch culture (Wilkinson, 1988).…”

Section: Effects Of Growth Ratementioning

confidence: 99%

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Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

1998

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Burkho/deria cepacia produces an unusual range of polar lipids, which includes two forms each of phosphatidylethanolamine (PE) and ornithine amide lipid (OL), differing in the presence or absence of 2-hydroxy fatty acids. By using chemostat cultures in chemically defined media, variations in the lipid content and the proportions of individual lipids have been studied as a function of (a) growth temperature, (b) growth rate and (c) growth-limiting nutrient (carbon, magnesium, phosphorus or oxygen). Total cellular lipid in carbon-limited cultures was lowest at high growth temperatures and low growth rates. Increases in growth temperature over the range 2540 O C led t o increases in the proportions of molecular species of PE and OL containing 2-hydroxy acids, without changing the PE:OL ratio. Growth temperature did not alter the balance between neutral and acidic lipids, but the contribution of phosphatidylglycerol to the latter increased with rising growth temperature and growth rate. Pigmentation of cells and the presence of flagella were also temperature-dependent. Change in growth rate also affected the PE :OL ratio and the extent to which monoenoic acids were replaced by their cyclopropane derivatives. Whereas similar lipid profiles were found for carbon-, magnesiumand oxygen-limited cultures, ornithine amides were the only polar lipids detected in phosphorus-limited cells.

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Section: Effects Of Growth Ratementioning

confidence: 73%

Section: Effects Of Growth Ratementioning

confidence: 99%

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

1998

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“…A higher proportion of trans acids was noted in Ms. trichosporium by Makula [18] than in our analysis. Variations in the methodology or more probably culture conditions [31,32] may account for this trend.…”

Section: Discussionmentioning

confidence: 99%

Phospholipid and lipopolysaccharide normal and hydroxy fatty acids as potential signatures for methane-oxidizing bacteria

Nichols¹,

A²,

Antworth³

et al. 1985

FEMS Microbiology Letters

131

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The extractable ester‐linked and the lipopolysaccharide (LPS) normal and hydroxy fatty acids of the methylotrophic bacteria Methylosinus trichosporium 0B3B, Methylobacterium organophilum XX, grown on methane and methanol, Mb. organophilum RG and Methylomonas sp. were analysed by capillary gas chromotography‐mass spectrometry (GC‐MS). Precise monounsaturated double bond position and geometry was determined by GC‐MS analysis of the derivatized fatty acids. The three species were readily distinguished based on the extractable fatty acid and LPS hydroxy acid profiles. Type I and Type II methylotrophs can be separated based on the presence of 16‐carbon and 18‐carbon monoenoic fatty acids in the two groups of organisms, respectively. Relatively novel components, 18: 1ω8c, 18: 1ω8t, 18: 1ω7t and 18: 1ω6c were present in Ms. trichosporium, and 16: 1ω8c, 16: 1ω8t, 16: 1ω7t, 16: 1ω5c and 16: 1ω5t were detected in Methylomonas sp. These specific lipids may be used, together with other components, as signatures for these methylotrophic bacteria in manipulated laboratory and environmental samples.

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“…Variations in membrane composition can be brought about by changes in the genetic makeup (1,18,21,39,46) or by nutritional factors (13,16,21,24,25,47), which can result in dramatic differences in sensitivity of these organisms to a variety of stresses (13,18,24,46). This might help to explain the variability in sensitivity and resistance of gram-negative bacteria in stressful environments such as water bodies, chlorinetreated water, frozen food, and other environmental samples where organisms with injured walls can be detected (7,9,19,44,50).…”

Section: Resultsmentioning

confidence: 99%

Effect of defined lipopolysaccharide core defects on resistance of Salmonella typhimurium to freezing and thawing and other stresses

Bennett¹,

Seaver²,

Calcott³

1981

Appl Environ Microbiol

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A family of mutants of Salmonella typhimurium with altered lipopolysaccharide (LPS) core chain lengths were assessed for sensitivity to freeze-thaw and other stresses. Deep rough strains with decreased chain length in the LPS core were more susceptible to novobiocin, polymyxin B, bacitracin, and sodium lauryl sulfate during growth, to ethylenediaminetetraacetic acid and sodium lauryl sulfate in resting suspension, and to slow and rapid freeze-thaw in water and saline, and these strains exhibited more outer membrane damage than the wild type or less rough strains. Variations in the LPS chain length did not dramatically affect the sensitivity of the strains to tetracycline, neomycin, or NaCl in growth conditions or the degree of freeze-thaw-induced cytoplasmic membrane damage. The deeper rough isogenic strains incorporated larger quantities of less-stable LPS and less protein into the outer membrane than did the wild type or less rough mutants, indicating that the mutations affected outer membrane synthesis or organization or both. Nikaido's model of the role of LPS and protein in determining the resistance of gram-negative bacteria to low-molecular-weight hydrophobic antibiotics is discussed in relation to the stress of freeze-thaw. The stress of freezing and thawing causes considerable damage to microbes, including loss of viability, membrane damage, deoxyribonucleic acid damage, ultrastructural changes, and loss of respiratory, active uptake, nutrient retention, and protein synthesis activities (3

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Phenotypic Variability of Lipids of Escherichia Coli Grown in Chemostat Culture

Cited by 6 publications

References 12 publications

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

Phospholipid and lipopolysaccharide normal and hydroxy fatty acids as potential signatures for methane-oxidizing bacteria

Effect of defined lipopolysaccharide core defects on resistance of Salmonella typhimurium to freezing and thawing and other stresses

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