Phenotypic variability in human embryonic holoprosencephaly in the Kyoto Collection

Yamada, Shigehito; Uwabe, Chigako; Fujii, Shingo; Shiota, Kohei

doi:10.1002/bdra.20048

Cited by 87 publications

(112 citation statements)

References 48 publications

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“…Approximately 44,000 human embryos, comprising the ''Kyoto collection,'' are historical specimens collected and stored at the Congenital Anomaly Research Center of Kyoto University (Nishimura et al, 1968;Nishimura, 1975;Shiota, 1991;Yamada et al, 2004). In most cases, pregnancy was terminated during the first trimester of pregnancy for socioeconomic reasons under the Maternity Protection Law of Japan.…”

Section: Materials and Methods Human Embryo Specimensmentioning

confidence: 99%

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Hirose

Nakashima

Yamada

et al. 2011

The Anatomical Record

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Embryonic liver has a unique external morphology and quantitative morphometry, based on magnetic resonance imaging data of human embryos from the Kyoto Collection of Human Embryos. Liver morphogenesis is strongly affected by the adjacent organs and tissues. The left ventricle develops to the left medial-caudal side, which results in the formation of a depression at left medial region and a prominence bilaterally at the cranial surface of the liver between Carnegie Stage (CS)17 and CS19. An imprint of the stomach that formed at the dorsal left-medial region of the liver became more marked with development until CS23. A depression induced by the umbilicus formed at the ventral region of the liver between CS16 and CS19. An indentation caused by the right adrenal gland formed at the dorsal-caudal region of the liver surface from CS20. Morphometric analysis revealed that the volume of the liver increased exponentially from CS14 through CS23. The liver developed preferentially along the dorsoventral axis and right/left axis until CS17, along the craniocaudal axis between CS17 and CS19, and then in all directions after CS19. Several important developmental phenomena, such as differentiation of the diaphragm, the extension of the body axis of the embryo, and the physiologic herniation of the intestine into the umbilical cord, may affect morphometric data. These data contribute to a better understanding of liver development as well as the morphogenesis of adjacent organs, both temporally and spatially, and serve as a useful reference for fetal medicine and prenatal diagnosis. Anat Rec, 295:51-59, 2012. V V C 2011 Wiley Periodicals, Inc.

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Section: Materials and Methods Human Embryo Specimensmentioning

confidence: 99%

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Hirose

Nakashima

Yamada

et al. 2011

The Anatomical Record

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“…The specimens were in fixative for an estimated duration of 30 to over 40 years, making them unsuitable for immunohistochemistry or any molecular/cellular analysis. This collection represents a random sample of the total intrauterine population of Japan [13][14][15][16] . During accessioning into the Kyoto collection, the embryos were examined and staged according to the criteria of Carnegie Staging proposed by O'Rahilly 17 .…”

Section: Specimensmentioning

confidence: 99%

“…The complex morphogenetic changes occurring during human heart development were examined using magnetic resonance imaging (MRI) and episcopic fluorescence image capture (EFIC). This analysis included 52 human embryos and spanned 6 4 / 7 -9 3 / 7 weeks estimated gestational age (EGA), corresponding to Carnegie stages (CS) [13][14][15][16][17][18][19][20][21][22][23]. Serial two-dimensional image stacks and three-dimensional reconstructions allowed analysis of external morphology and internal structures of the heart.…”

Section: Introductionmentioning

confidence: 99%

Cardiovascular Development in the First Trimester

Dhanantwari¹,

Leatherbury²,

Lo³

2012

The Human Embryo

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“…and the embryo collection can be considered to be representative of the total intrauterine population in Japan. Further details of the Kyoto Collection of Human Embryos have been described in previous reports (Nishimura et al, 1968;Nishimura, 1975;Shiota, 1991;Yamada et al, 2004). The embryo collection now comprises over 44,000 specimens and approximately 20% are undamaged well-preserved embryos.…”

Section: Experimental Procedures Embryo Specimensmentioning

confidence: 99%

Graphic and movie illustrations of human prenatal development and their application to embryological education based on the human embryo specimens in the Kyoto collection

Yamada

Uwabe

Nakatsu-Komatsu

et al. 2005

Developmental Dynamics

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Morphogenesis in the developing embryo takes place in three dimensions, and in addition, the dimension of time is another important factor in development. Therefore, the presentation of sequential morphological changes occurring in the embryo (4D visualization) is essential for understanding the complex morphogenetic events and the underlying mechanisms. Until recently, 3D visualization of embryonic structures was possible only by reconstruction from serial histological sections, which was tedious and time-consuming. During the past two decades, 3D imaging techniques have made significant advances thanks to the progress in imaging and computer technologies, computer graphics, and other related techniques. Such novel tools have enabled precise visualization of the 3D topology of embryonic structures and to demonstrate spatiotemporal 4D sequences of organogenesis. Here, we describe a project in which staged human embryos are imaged by the magnetic resonance (MR) microscope, and 3D images of embryos and their organs at each developmental stage were reconstructed based on the MR data, with the aid of computer graphics techniques. On the basis of the 3D models of staged human embryos, we constructed a data set of 3D images of human embryos and made movies to illustrate the sequential process of human morphogenesis. Furthermore, a computer-based self-learning program of human embryology is being developed for educational purposes, using the photographs, histological sections, MR images, and 3D models of staged human embryos. Developmental Dynamics 235:468 -477, 2006.

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Phenotypic variability in human embryonic holoprosencephaly in the Kyoto Collection

Abstract: The early development of HPE in human embryos was systematically studied for the first time. The pathogenesis of craniofacial abnormalities, especially eye anomalies, in HPE was discussed in the light of recent studies with mutant laboratory animals.

Cited by 87 publications

References 48 publications

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Embryonic Liver Morphology and Morphometry by Magnetic Resonance Microscopic Imaging

Cardiovascular Development in the First Trimester

Graphic and movie illustrations of human prenatal development and their application to embryological education based on the human embryo specimens in the Kyoto collection

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