Phenotypic, structural, and ultrastructural analysis of triple-negative breast cancer cell lines and breast cancer stem cell subpopulation

Moreira, Milene Pereira; Brayner, Fábio André; Alves, Luíz Carlos; Cassali, Geovanni Dantas; Silva, Luciana Maria

doi:10.1007/s00249-019-01393-0

Cited by 7 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to biochemical heterogeneity observed within CSCs (Bocci, Gearhart-Serna et al 2019, Moreira, Brayner et al 2019), CSCs can be biophysically strikingly different too In a recent study, Chen et al, showed the association of biophysical properties of CSCs and their ability to invade, migrate, and initiate tumors, using the SUM149 inflammatory breast cancer (IBC) stem cells (Chen, Allen et al 2019). In this study, the authors sorted SUM149 CSCs based on the expression of aldehyde dehydrogenase enzyme (ALDH) and assayed biophysical properties of ALDH+ and ALDH-cells in terms of deformability, adhesion strength and contractility.…”

Section: Biophysical Characterization Of Cscs and Their Subsetsmentioning

confidence: 62%

Cancer Stem Cell Plasticity – A Deadly Deal

Archana¹,

Kritika²,

Murali³

et al. 2019

Preprint

View full text Add to dashboard Cite

Intratumoral heterogeneity is a major ongoing challenge in the effective therapeutic targeting of cancer. Accumulating evidence suggests that a fraction of cells within a tumor termed Cancer Stem Cells (CSCs) are primarily responsible for this diversity resulting in therapeutic resistance and metastasis. Adding to this complexity, recent studies have shown that there can be different subpopulations of CSCs with varying biochemical and biophysical traits resulting in varied dissemination and drug-resistance potential. Moreover, cancer cells can exhibit a high level of plasticity or the ability to dynamically switch between CSC and non-CSC states or among different subsets of CSCs. The molecular mechanisms underlying such plasticity has been under extensive investigation and the trans-differentiation process of Epithelial to Mesenchymal transition (EMT) has been identified as a major contributing factor. Besides genetic and epigenetic factors, CSC plasticity is also shaped by non-cell-autonomous effects such as the tumor microenvironment. In this review, we discuss the recent developments in understanding CSC plasticity in tumor progression at biochemical and biophysical levels, and the latest in silico approaches being taken for characterizing cancer cell plasticity with implications in improving existing therapeutic approaches.

show abstract

Section: Biophysical Characterization Of Cscs and Their Subsetsmentioning

confidence: 62%

Cancer Stem Cell Plasticity – A Deadly Deal

Archana¹,

Kritika²,

Murali³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The strongest evidence of this sponging effect comes from the first discovery of the binding of CDR1as to an miRNA that contains sequences matching 74 miR-7 seeds and is tightly bound by the AGO protein (miRNA-binding protein), which strongly inhibits the activity of miR-7 in nerve tissue and leads to an increase in the miR-7 targeting level, thus affecting the development of tumors ( 31 , 39 ). CircRNAs regulate miRNA expression in the cytoplasm by acting as miRNA sponges and absorbing miRNAs or other targets to regulate tumor metabolism ( 7 , 40 , 41 ). The adsorption of miRNA by circRNA results in the formation of the circRNA/miRNA/mRNA regulatory axis by reducing miRNA expression and releasing mRNA from functional inhibition ( 42 ) ( Figure 1A ).…”

Section: The Functions Of Circrnasmentioning

confidence: 99%

“…TNBC is responsible for 15%–20% of all breast cancers and remains the most challenging subtype of breast cancer. For a specific molecular target therapy in the prognosis of patients with TNBC, there is seldom clinically meaningful improvement; therefore, TNBC is one of the worst prognosis among breast cancer subtypes of all types due to the high propensity for progression, difficulty of identifying effective targets, and lack of specific targeted therapy, leading to a greater recurrence and metastasis potential and shorter overall survival ( 7 , 8 ). The heterogeneity of breast cancer causes great confusion in diagnosis and treatment and affects the survival rate, treatment response, and tumor growth rate ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

The Biogenesis and Functions of circRNAs and Their Roles in Breast Cancer

et al. 2021

View full text Add to dashboard Cite

Recent statistics show that breast cancer is among the most frequent cancers in clinical practice. It is also the second-leading cause of cancer-related deaths among women worldwide. CircRNAs are a new class of endogenous regulatory RNA molecules whose 5’ end and 3’ end are connected together to form a covalently closed single-stranded loop by back-splicing. CircRNAs present the advantages of disease-specific expression and excellent expression stability, and they can modulate gene expression at posttranscriptional and transcriptional levels. CircRNAs are abnormally expressed in multiple cancers, such as breast cancer, and drive the initiation and progression of cancer. In this review, we describe current knowledge about the functions of circRNAs and generalize their roles in various aspects of breast cancer, including cell proliferation, cell cycle, apoptosis, invasion and metastasis, autophagy, angiogenesis, drug resistance, and tumor immunity, and their prognostic and diagnostic value. This may add to a better understanding of the functions and roles of circRNAs in breast cancer, which may become new diagnostic and predictive biomarkers of breast cancer.

show abstract

“…Conventional chemotherapy preferentially targets proliferating cells which can leave behind resistant stem-like cells, which can cause recurrence [ 9 ]. Within the TNBC cell lines BT-549 and Hs 578T, there are three different subpopulations [ 10 ]: (1) the CD44 + CD24 − cells characterise the breast cancer stem mesenchymal-like phenotype (cancer stem cells, CSC), (2) the CD44 − CD24 + cells are associated with epithelial cells and are largely present in distant metastases [ 11 ], and (3) the CD44 + CD24 + cells with mesenchymal and epithelial features are plastic and able to self-renew [ 12 ]. Due to the different signalling pathways in epithelial and mesenchymal cancer stem populations, they respond differently to treatments.…”

Section: Introductionmentioning

confidence: 99%

Thieno[2,3-b]Pyridine Derivative Targets Epithelial, Mesenchymal and Hybrid CD15s+ Breast Cancer Cells

et al. 2021

View full text Add to dashboard Cite

The adhesion of cancer cells to vascular endothelium is a critical process in hematogenous metastasis and might be similar to the recruitment of leukocytes at the site of inflammation. It is mediated by E-selectin and its ligands, of which the most stereospecific is a glycoconjugate sialyl Lewis x (CD15s), which may be expressed as an oligosaccharide branch of the CD44 glycoprotein, as well as a self-contained glycosphingolipid. It is also known that increased sialylation of glycoconjugates is a feature of malignant cells. The aim of the study was to analyse the effect of a novel thieno[2,3-b]pyridine, compound 1, in MDA-MB-231 triple-negative breast cancer cells (TNBCs) upon CD15s and CD44 expression in different cell subpopulations using flow cytometry. CD15s expression was compared between mesenchymal-like cancer stem cells (CSC, CD44+CD24−), epithelial cells without CD44 (CD44−CD24+ and CD44−CD24−), and CD44+CD24+ cells that exhibit mesenchymal and epithelial features. In addition, expression of CD44 in CD15s+CSC and CD15s−CSC was determined. Compound 1 significantly decreased the percentage of CD15s+CSC, CD15s+CD44+CD24+, and CD15s+CD44− subpopulations, as well as the expression of CD15s in CD44+CD24+ and CD44− cells, and therefore shows potential as a treatment for TNBC.

show abstract

Phenotypic, structural, and ultrastructural analysis of triple-negative breast cancer cell lines and breast cancer stem cell subpopulation

Cited by 7 publications

References 48 publications

Cancer Stem Cell Plasticity – A Deadly Deal

Cancer Stem Cell Plasticity – A Deadly Deal

The Biogenesis and Functions of circRNAs and Their Roles in Breast Cancer

Thieno[2,3-b]Pyridine Derivative Targets Epithelial, Mesenchymal and Hybrid CD15s+ Breast Cancer Cells

Contact Info

Product

Resources

About

Phenotypic, structural, and ultrastructural analysis of triple-negative breast cancer cell lines and breast cancer stem cell subpopulation

Cited by 7 publications

References 48 publications

Cancer Stem Cell Plasticity &ndash; A Deadly Deal

Cancer Stem Cell Plasticity &ndash; A Deadly Deal

The Biogenesis and Functions of circRNAs and Their Roles in Breast Cancer

Thieno[2,3-b]Pyridine Derivative Targets Epithelial, Mesenchymal and Hybrid CD15s+ Breast Cancer Cells

Contact Info

Product

Resources

About

Cancer Stem Cell Plasticity – A Deadly Deal

Cancer Stem Cell Plasticity – A Deadly Deal