Phenotypic properties, interleukin 2 production, and developmental origin of a "mature" subpopulation of Lyt-2- L3T4- mouse thymocytes.

Abstract

Two-color flow microfluorometry using monoclonal antibodies to cell surface determinants has shown that a subpopulation of mouse Lyt-2-L3T4-thymocytes, comprising 18% of Lyt-2-L3T4-cells in adult C57BL/6 mice, appears in the thymus late during fetal development. These Lyt-2-L3T4-cells are characterized by lack of expression of determinants recognized by monoclonal antibody Jild, a phenotype characteristic of more "mature" functional T cells. This J11d-subpopulation of Lyt-2-L3T4-thymocytes has now been shown to produce significant quantities of interleukin 2 following mitogen stimulation and to express T-cell receptor molecules recognized by monoclonal antibodies KJ-16 and F23

Distribution of α4β7 and αEβ7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes

“…3). Previously, the markers HSA, IL-2R, TCR and CD44 have been shown to subdivide this population into thymocytes with and without pluripotent potential [25] and P7 may also prove useful in this area. Since P7 is co-expressed on TCR' thymocytes, the P7+ CD4-CD8-thymocytes may correspond to a previously defined lineage of CD3' CD4-CD8-thymocytes [26,27].…”

NKT cells: In the beginning…

“…By pure serendipity these mAb were both directed against the Vb8 domain that is dramatically over-represented among iNKT cells. Thus it rapidly became clear that the DN thymocyte subset had a TCR repertoire that was heavily biased towards Vb8 [1][2][3].…”

“…A third report followed shortly thereafter [3]. Around the same time an unusual invariant TCR Va14-Ja18 rearrangement was cloned from a panel of keyhole limpet hemocyaninspecific suppressor T cell hybridomas [4,5].…”

“…By pure serendipity these mAb were both directed against the Vb8 domain that is dramatically over-represented among iNKT cells. Thus it rapidly became clear that the DN thymocyte subset had a TCR repertoire that was heavily biased towards Vb8 [1][2][3].With hindsight it is interesting to note that this first glimpse of iNKT cells was interpreted incorrectly by both groups that made the initial discovery. Based on the dogma that DN thymocytes were immature, we speculated that the Vb8-biased subset might arise from preferential usage of certain Vb domains early in development [1] …”

B and T Cell antigen receptor repertoires in lupus/arthritis murine models

“…To address whether the Igk gene complex in lupus mice harbors abnormalities that might contribute to autoantibody formation, and to determine possible associations of murine lupus with a particular Igk haplotype, we subjected genomic DNA from all major lupus strains and their progenitors (as far as known) to RFLP analysis with our probe panel (Vkl,2,(4)(5)8,9,10,11,(12)(13)14,19,21,22,23,24,28, VkRF, and Jk/Ck). As detailed elsewhere [62], the Vk loci of autoimmune ~k HAPLO TYPE PROTO-TYPE STRAIN (11 24 A.N.…”