Phenotypic markers for forensic purposes

Freire-Aradas, Ana; Phillips, Christopher; Huidobro, Victoria Lareu; Carracedo, Ángel

doi:10.1002/9781119482062.ch29

Cited by 2 publications

(3 citation statements)

References 60 publications

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“…This is due to the extensive DNA fragmentation and loss that occurs during the bisulfite conversion process and is problematic for forensic type samples. Currently, some laboratories are testing low template input material to determine if the quantity of input DNA required for accurate and reliable methylation analysis can be reduced . However, recent studies have highlighted the influence of stochastic effects on the methylation results of low template samples .…”

Section: Massively Parallel Sequencingmentioning

confidence: 99%

“…Epigenomic‐based lifestyle predictions and the biogeographic ancestry of an individual have also been hypothesized as potential applications in the future . A comprehensive summary of all potential applications is not within the scope of this review but has been covered in other articles .…”

Section: Applications Of Forensic Dna Methylation Profilingmentioning

confidence: 99%

“…Multiplex amplifications are also feasible, although primer design can be challenging due to the nature of bisulfite treated DNA (described below). This capability overcomes a significant limitation of pyrosequencing, where PCR amplification and sequencing are typically performed as single reactions and multiplex primer design (required for both amplification and sequencing steps) is often complex .…”

Section: Massively Parallel Sequencingmentioning

confidence: 99%

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Evaluation of massively parallel sequencing for forensic DNA methylation profiling

et al. 2018

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Epigenetics is an emerging area of interest in forensic science. DNA methylation, a type of epigenetic modification, can be applied to chronological age estimation, identical twin differentiation and body fluid identification. However, there is not yet an agreed, established methodology for targeted detection and analysis of DNA methylation markers in forensic research. Recently, a massively parallel sequencing-based approach has been suggested. The use of massively parallel sequencing is well established in clinical epigenetics and is emerging as a new technology in the forensic field. This review investigates the potential benefits, limitations, and considerations of this technique for the analysis of DNA methylation in a forensic context. The importance of a robust protocol, regardless of the methodology used, which minimizes potential sources of bias is highlighted.

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Section: Massively Parallel Sequencingmentioning

confidence: 99%

Section: Applications Of Forensic Dna Methylation Profilingmentioning

confidence: 99%

Section: Massively Parallel Sequencingmentioning

confidence: 99%

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Evaluation of massively parallel sequencing for forensic DNA methylation profiling

et al. 2018

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The professional practice of forensic anthropology: Contemporary developments and cross‐disciplinary applications

Franklin

Marks

2021

WIREs Forensic Science

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The modern practice of forensic anthropology is focused on the quantification of skeletal variation based upon a qualitative prowess developed according to theory and method in the parent discipline of biological anthropology. Such morphological variances allow estimates of biological attributes, for example, age, sex, population affinity, stature, pathology of disease processes-among others, that collectively may resolve the identification of unknown forensic human remains.The latter is a basic human right under International Humanitarian Law (IHL), and International Human Rights Law (IHRL) afforded to descendants who go missing in armed conflicts and other violence-base situations. The specific traditional methods applied by the forensic anthropologist are well established in the published literature and routinely supported by global research that guides graduate programs designed to train the next generation of practitioners. Important in that cornerstone literature and graduate training regimen are aspects of our practice that are increasingly cross-disciplinary, especially those grounded in the molecular, chemical, and histological sciences. These upper-tier endeavors supplement and many times, validate, the traditional approaches as dictated by case context. This review focuses on evolving cross-disciplinary developments and novel research trends in the analysis of human remains that involve the specific expertise of the forensic anthropologist.

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Phenotypic markers for forensic purposes

Cited by 2 publications

References 60 publications

Evaluation of massively parallel sequencing for forensic DNA methylation profiling

Evaluation of massively parallel sequencing for forensic DNA methylation profiling

The professional practice of forensic anthropology: Contemporary developments and cross‐disciplinary applications

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