Phenotypic dissection of cord blood immunoregulatory T-cell subsets by using a two-color immunofluorescence study

Gerli, Roberto; Bertotto, A; Spinozzi, Fabrizio; Cernetti, C; Grignani, F; Rambotti, P

doi:10.1016/0090-1229(86)90187-x

Cited by 37 publications

(15 citation statements)

References 28 publications

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Section: Cell Isolation Phenotypic Analysis and Total Rna Extractionmentioning

confidence: 99%

“…This procedure has been reported in detail previously (16). Negative controls and tests to prove the specificity of the isotypespecific Abs were performed for each experiment as previously described (16). In some experiments, FITC-labeled CD30 ϩ T cells were isolated by cell sorting (FACSCalibur, Becton Dickinson) for intracytoplasmic cytokine evaluation.…”

Section: Cell Isolation Phenotypic Analysis and Total Rna Extractionmentioning

confidence: 99%

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CD30+ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role

Gerli

Pitzalis

Bistoni

et al. 2000

The Journal of Immunology

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High serum levels of soluble CD30 (sCD30) have been reported to better predict the response to second line therapy in rheumatoid arthritis (RA). It is believed that sCD30 is released by CD30+ T cells present in the RA synovium. However, both the mechanism of recruitment to the joint and the functional role of this T cell subset in the pathogenesis of the disease remain unknown. This study confirmed higher levels of sCD30 in the serum and synovial fluid (SF) of RA patients compared with normal controls. However, analysis of mRNA and cell surface CD30 expression showed that CD30+ T cells are detectable in the SF, but not in the synovial membrane. In contrast, T cells expressing the CD30 transcript, but not the surface molecule, were found in the peripheral blood of both RA and normal controls. CD30 surface expression was up-regulated by adhesion and migration through endothelium in vitro and in a delayed-type hypersensitivity model in vivo. Although the great majority of fresh or cloned CD30+ T cells from SF produced both IFN-γ and IL-4, CD30 expression strictly correlated with IL-4 synthesis in synovial T cell clones. In addition, CD30+ T cell clones also produced high amounts of the anti-inflammatory cytokine IL-10. On this basis, we would like to propose that synovial CD30+ cells may play a role in the control of the inflammatory response. Serum sCD30 may reflect such cell activity and, therefore, explain the previously demonstrated correlation between high sCD30 serum levels and positive response to therapy.

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Section: Cell Isolation Phenotypic Analysis and Total Rna Extractionmentioning

confidence: 99%

Section: Cell Isolation Phenotypic Analysis and Total Rna Extractionmentioning

confidence: 99%

CD30+ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role

Gerli

Pitzalis

Bistoni

et al. 2000

The Journal of Immunology

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“…To test the capacity of the anti-Tl 12 MAb to induce the expression of the T113 membrane antigen, it was added alone to three anti-CD2-unresponsive pSS and three control PBMC samples. The results showed that the anti-TI 12 MAb induced an equal expression intensity of TI 13 on a similar number of T cells in both pSS and control PBMC samples (data not shown).…”

Section: Resultsmentioning

confidence: 73%

“…PBMC were isolated from heparinized peripheral blood by density gradient centrifugation on Ficoll-Hypaque (Lymphoprep; Nycomed AS, Oslo, Norway) and resuspended in RPMI-1640 supplemented with 10% FCS, 4 mM L-glutamine, 100 U/ml penicillin, and 100 gg/ml streptomycin (complete medium) (Gibco Laboratories, Grand Island, NY). PBMC were separated into sheep red blood cell rosette-enriched (E+) and rosette-depleted (E-) subpopulations as detailed elsewhere (12). E--cell suspensions were then irradiated at 3,000 rad (E-) and used as a source of accessory cells (AC standard curve, constructed by using serial dilutions of rIL-2 of known activity, was used to calculate the concentration of IL-2, which was expressed as U/ml in each culture supernatant.…”

Section: Methodsmentioning

confidence: 99%

Basis for defective proliferation of peripheral blood T cells to anti-CD2 antibodies in primary Sjögren's syndrome.

Gerli¹,

Bertotto²,

Agea³

et al. 1990

J. Clin. Invest.

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“…The analysis of human cord blood lympho cytes using different monoclonal antibodies showed significant differences in the marker distribution on T helper, T suppressor and B lymphocytes between newborns and adults [15][16][17][18], In this study we focused on the NK and cytotoxic cell phenotype in neonatal blood. Using two-color flow cytometric analy sis, we found a significant decrease in the pro portions of precursors and effectors of allocytotoxic T cells (CD 8+CD llb-) in neonatal blood, but no difference was noted for the absolute numbers of these cells.…”

Section: Discussionmentioning

confidence: 99%

Two-Color Flow Cytometric Analysis of Natural Killer and Cytotoxic T-Lymphocyte Subsets in Peripheral Blood of Normal Human Neonates

Slukvin

Chernishov²

1992

Neonatology

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It has been demonstrated that normal human newborns had significantly lower percentages of CD8+, CD11b+, CD 16+ and CD 56+, compared to adults, while no differences had been noted for the absolute numbers of these cells. Both the proportions and absolute numbers of CD 5 7+ lymphocytes and cytotoxic T cells that mediate non-MHC-restricted cytotoxicity (CD3+CD56+) were markedly reduced in neonatal blood. Mean proportions but not absolute numbers of precursors and effectors of allocytotoxic T cells (CD8+CD11b––) significantly decreased in human neonates. Dual-labeling experiments demonstrated that newborns’ natural killer cells had a phenotype different from that of adults. In newborns, there were much higher levels of CD1 1b+ cells, much lower levels of CD57+ cells and similar levels of CD8+ lymphocytes among CD 16+ lymphocytes. It seems that a phenotype immaturity of natural killer cells may contribute to the diminished cytotoxic potential of neonatal lymphocytes.

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Phenotypic dissection of cord blood immunoregulatory T-cell subsets by using a two-color immunofluorescence study

Cited by 37 publications

References 28 publications

CD30+ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role

CD30+ T Cells in Rheumatoid Synovitis: Mechanisms of Recruitment and Functional Role

Basis for defective proliferation of peripheral blood T cells to anti-CD2 antibodies in primary Sjögren's syndrome.

Two-Color Flow Cytometric Analysis of Natural Killer and Cytotoxic T-Lymphocyte Subsets in Peripheral Blood of Normal Human Neonates

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