Phenotypic Detection of Extended Spectrum Beta-Lactamase and Carbapenemases Produced by Klebsiella spp Isolated from Three Referrals Hospitals in Yaounde, Cameroon

Betbeui, Anicette Chafa; Kamga, Hortense Gonsu; Toukam, Michel; Mbakop, Calixte Didier; Lyonga, Emilia; Bilong, Serge; Koulla‐Shiro, Sinata

doi:10.9734/bmrj/2015/18250

Cited by 7 publications

(4 citation statements)

References 21 publications

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“…The interplay of the genetic disposition of K. pneumoniae makes it better organized for mobilizing resistant genes from other drug resistant bacteria in the environment or in animal/human microbial communities. This further explains why K. pneumoniae is always the predominant species as was the case in an earlier study (19). Such information is fundamental for shaping effective routine analysis of clinical specimens.…”

Section: Discussionsupporting

confidence: 58%

Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon

Lyonga Mbamyah,

Kumcho,

Michel

et al. 2022

RAS Microbiology and Infectious Diseases

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Background: Carbapenemases are the most potent beta-lactamases capable of hydrolysing all beta-lactam drugs. Data scarcity on laboratory evidence of carbapenemase producers obstructs infection prevention and effective control measures. This study aimed to detect and phenotypically characterize Klebsiella species resistant to carbapenems. Method: A total of eighty-seven (87) Klebsiella spp. isolates were collected from three Yaounde-based tertiary hospitals over a period of twelve months (February 2019 to January 2020). The isolates were identified on API 20E and antimicrobial susceptibility testing was done according to the Kirby Bauer disc diffusion method. Carbapenem resistance was detected based on reduction in inhibition zone diameter of meropenem less than 28mm and resistance phenotypes determined by the combination disc synergy test. Results: Four Klebsiella species were identified namely: Klebsiella pneumoniae 62/87 (71.3%), K. oxytoca 11/87 (12.6%), K. ozaenae 7/87 (8%), K. rhinoscleromatis 7/87 (8%). Klebsiella pneumoniae was most resistant to carbapenems with a considerably high number of multidrug resistant isolates 39/56 (69.6%). Patients from the 0-9 years age group were most represented 29/87 (33.3%). Carbapenemases belonging to Ambler classes A, B and D were detected as follows: KPC 2/87 (2.3%), MBL 2/87(2.3%), OXA-48 4/87 (4.6%). All four Klebsiella species were producers of KPC carbapenemase 12/87 (13.8%) and Extended Spectrum β-lactamase 68/87 (78.2%) enzymes. Conclusion: These results revealed that more than half of Klebsiella species analysed were multidrug resistant and about a quarter in hospitalized patients at the referral hospitals were mostly KPC carbapenemase producers. This raises concerns over the need to control and confine the spread of these isolates of clinical and epidemiological importance for effective infection prevention and control.

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Section: Discussionsupporting

confidence: 58%

Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon

Lyonga Mbamyah,

Kumcho,

Michel

et al. 2022

RAS Microbiology and Infectious Diseases

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“…This result is different from results reported by Betbeui and collaborators in 2015 from three referral hospitals in Yaounde. The study reported lower rates of resistance for the cephalosporins [16]. Thus there has been an increase over time in resistance to cephalosporins.…”

Section: Discussionmentioning

confidence: 78%

“…Thirty seven Klebsiella isolates were identified using the API 20E identification system. Among the species isolated Klebsiella pneumoniae was the most prevalent with 54.1%, followed by Klebsiella rhinoscleromatis 18.9%, Klebsiella ozaenae 16.2% and Klebsiella oxytoca, 10.8% as shown on Figure 1 below. Klebsiella species were most isolated from pus 24.3%, urinary catheter and urine with a frequency of 21.6% each and isolated least from venous catheter 5.4% as shown on Table 1.…”

Section: Resultsmentioning

confidence: 91%

High Prevalence of Multidrug Resistant <i>Klebsiella</i> Species Isolated from the Yaounde University Teaching Hospital, Cameroon

Lyonga¹,

Enyeji²,

Torimiro³

et al. 2021

OJMM

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Background and Purpose: Klebsiella species are amongst the most common causes of a variety of community-acquired and hospital-acquired infections (HAI), characterized by high morbidity and mortality rates. Most infections caused by Klebsiella species are usually treated using antibiotics. The aim of this study was to determine the antimicrobial resistance profile of Klebsiella species isolated from in-patients and out-patients at the Yaounde University Teaching Hospital. The data generated will go a long way to improve on the choice of an adequate empiric antibiotic treatment for infections caused by Klebsiella species. Methodology: A cross-sectional descriptive study was carried out over a period of 6 months, spanning from February 2019 to July 2019 with a sample size of 37 isolates, obtained from 6 different clinical specimens. Identification of isolates was done using API 20E identification system (Biomerieux SA, Lyon, France). Susceptibility to antibiotics was tested as described by Kirby-Bauer in 1956. Inhibition diameters were interpreted according to recommendations from the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2019). Results and Conclusion: Among the 37 Klebsiella isolates identified, Klebsiella pneumoniae was the most prevalent species isolated with a percentage of 54.1%, followed by Klebsiella rhinoscleromatis 18.9%, Klebsiella ozaenae 16.2% and Klebsiella oxytoca, 10.

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“…The low rate of CP-GNB clinical samples is similar to that reported by a study in Chad (2.5%), 4 but is less than in Cameroon (Central Africa; 11.11%) and Senegal (West Africa; 5.1%). 22,23 Conversely, it is more than that in Burkina Faso (West Africa; 0.9%). 24 This low rate in Gabon could be explained by the high cost of carbapenems, which reduces the risk of excessive use, and therefore promotes the appearance of resistance.…”

Section: Discussionmentioning

confidence: 96%

Prevalence and Phenotypic and Molecular Characterization of Carbapenemase-Producing Gram-Negative Bacteria in Gabon

Dikoumba

Onanga

Jean-Pierre

et al. 2023

The American Journal of Tropical Medicine and Hygiene

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Data collection and monitoring of carbapenemase-producing (CP) Gram-negative bacteria (GNB) are often limited. This study determined CP-GNB prevalence in Gabon and the genetic origins of the resistance genes. From January 2016 to March 2018, 869 clinically significant GNB isolates from inpatients and outpatients, and 19 fecal samples (inpatients) were analyzed in the main hospitals of Gabon. Fecal samples were screened using ChromID® CARBA SMART selective chromogenic medium biplates. Species were identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry. Antibiotic susceptibility was tested using the disk diffusion method on Müller–Hinton agar, and resistance genes were assessed by multiplex polymerase chain reaction and sequencing. Overall, 1.61% of clinical isolates (14 of 869) and 5.26% of fecal samples (1 of 19) were CP-GNB. The CP-GNB rate was higher among inpatients (2.98%) than outpatients (0.33%), in intensive care units (28.57%, 4 of 14), and in urine samples (35.71%, 5 of 14). The most common CP-GNB were Klebsiella pneumoniae (53.33%) and Acinetobacter baumannii (26.67%). blaOXA-48 was the predominant carbapenemase-encoding gene (40%), followed by blaNDM-5 (33.33%). The A. baumannii multilocus sequence types ST2 and ST78, Enterobacter cloacae ST78, Escherichia coli ST2, and K. pneumonia ST48 and ST147 were found. These data indicate that CP bacteria are present in clinical and carriage samples. Preventive measures are needed to avoid the spread of resistance genes.

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Phenotypic Detection of Extended Spectrum Beta-Lactamase and Carbapenemases Produced by Klebsiella spp Isolated from Three Referrals Hospitals in Yaounde, Cameroon

Abstract: Aims:The main objective of this study was to determine the resistance phenotype of β-lactamines by Klebsiella in three hospitals in Yaoundé. Study Design: A cross-sectional descriptive study.

Cited by 7 publications

References 21 publications

Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon

Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon

High Prevalence of Multidrug Resistant <i>Klebsiella</i> Species Isolated from the Yaounde University Teaching Hospital, Cameroon

Prevalence and Phenotypic and Molecular Characterization of Carbapenemase-Producing Gram-Negative Bacteria in Gabon

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Phenotypic Detection of Extended Spectrum Beta-Lactamase and Carbapenemases Produced by Klebsiella spp Isolated from Three Referrals Hospitals in Yaounde, Cameroon

Abstract: Aims:The main objective of this study was to determine the resistance phenotype of β-lactamines by Klebsiella in three hospitals in Yaoundé. Study Design: A cross-sectional descriptive study.

Cited by 7 publications

References 21 publications

Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon

Carbapenemase Producing Klebsiella species from Hospitalised Patients at Three Referral Hospitals in Yaounde; Cameroon

High Prevalence of Multidrug Resistant &lt;i&gt;Klebsiella&lt;/i&gt; Species Isolated from the Yaounde University Teaching Hospital, Cameroon

Prevalence and Phenotypic and Molecular Characterization of Carbapenemase-Producing Gram-Negative Bacteria in Gabon

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High Prevalence of Multidrug Resistant <i>Klebsiella</i> Species Isolated from the Yaounde University Teaching Hospital, Cameroon