Phenotypic description and prevalence of Fasciola species in Qena Governorate, Egypt with special reference to a new strain of Fasciola hepatica

Hussein, Abdel-Nasser A.; Khalifa, R.

doi:10.1016/j.jksus.2009.12.001

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…eggs. A similar finding of 28.6% was reported by Hussain & Khalifa (2010) in Qena, Egypt. Our finding is also similar to those reported in Debre Zeit, Ethiopia (28.6%) (Abdulhakim & Addis, 2012) and in Nigeria (25.8%) (Negele & Ibe, 2014).…”

Section: Discussionsupporting

confidence: 90%

“…Morphologically, adult flukes in this study were identified as F. hepatica and this was augmented by the presence of F. hepatica larval stages in its intermediate host, G. truncatula . Several investigations based on morphological criteria, morphometric and chemotaxonomic data showed similar findings (Lotfy & Hillyer, 2003; Periago et al, 2008; Hussain & Khalifa, 2010). In this current study, mitochondrial DNA-targeting duplex PCR was used to identify Fasciola spp.…”

Section: Discussionsupporting

confidence: 59%

“…However, G. truncatula naturally infected with F. gigantica was reported (Dar et al, 2005, 2010) and under experimental conditions various snail species were successfully infected with F. hepatica (Dar et al, 2010, 2013, 2014). It is worth mentioning that in Egypt F. hepatica originated from imported animals (Lotfy et al, 2002; Mas-Coma et al, 2005; Hussain & Khalifa, 2010) and R. natalensis is considered to be its potential intermediate host (Dar et al , 2010). In the current study, no Fasciola- infected R. natalensis were identified among the 465 snails examined.…”

Section: Discussionmentioning

confidence: 99%

“…Fasciola hepatica (Linnaeus, 1758) has a wider range than its tropical counterpart, Fasciola gigantica (Cobbold, 1856), but their geographical distribution overlaps in many African and Asian countries (Mas-Coma et al, 2005). In Egypt, both species are present (Lotfy & Hillyer, 2003; WHO, 2007; Hussain & Khalifa, 2010; Dar et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Fasciola hepaticainfections in cattle and the freshwater snailGalba truncatulafrom Dakhla Oasis, Egypt

Arafa

Hassan²,

Snousi³

et al. 2017

J. Helminthol.

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Infection by Fasciola species was investigated in seven districts of Dakhla Oasis, Egypt, through abattoir inspection of cattle livers for adult worms and sedimentation of faecal samples from local cattle to detect Fasciola eggs. In addition, lymnaeid snails collected from the study area were examined microscopically for developmental stages of Fasciola spp. Abattoir inspection revealed that 51 out of 458 cattle livers (11.1%) contained adult flukes, which were identified morphologically as Fasciola hepatica. Examination of the cattle faecal samples revealed that 142 out of 503 (28.2%) contained Fasciola eggs. The collected snails, identified as Galba truncatula and Radix natalensis, showed larval stages of Fasciola in 71 out of 731 (9.7%) G. truncatula, while R. natalensis showed no infection. Specific duplex polymerase chain reaction (PCR) targeting the mitochondrial cox1 gene of F. hepatica and Fasciola gigantica was carried out on DNA extracted from pooled infected snails and adult worms. The F. hepatica size amplicon (1031 bp) was obtained from both the infected G. truncatula and the adult worms isolated from cattle livers from different districts. The amplicon sequences were identical to the published sequences of F. hepatica mitochondrial cox1 gene. In conclusion, the zoonotic importance of Fasciola infection and appropriate hygienic measures must be taken into consideration in Dakhla Oasis, Egypt.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fasciola hepaticainfections in cattle and the freshwater snailGalba truncatulafrom Dakhla Oasis, Egypt

Arafa

Hassan²,

Snousi³

et al. 2017

J. Helminthol.

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“…2009Roknil et al, 2010). It is a major zoonosis that causes a great deal of economic loss throughout the tropical region of the world (Gorokhov and Sergiev, 2008) and is an emerging public health prob-lem in the Middle East (Alcaino and Apt, 1989;Hussein andKhalifa, 2010, Hussein et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genetically Confirmed Fasciola Hepatogigantica N.SP.

Khalifa

El-Hady

OMRAN

et al. 2013

Journal of the Egyptian Society of Parasitology

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The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

Abdel-Rahman¹,

Mohamed

Abdel‐Rahman

et al. 2014

J Parasit Dis

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In the present study, the carbohydrate structures associated with Fasciola gigantica adult worm were identified by indirect hemagglutination inhibition test. Glucose was found to be the main monosaccharide associated with the fluke. According to indirect hemagglutination inhibition results, purification of glycoprotein fractions from worm crude extract was carried out by affinity chromatography immobilized glucose agarose gel and Con-A lectin columns. The isolated glycoprotein fractions, FI and FII, were characterized by SDS-PAGE which revealed one band in FI of 26 kDa and another one band of 19.5 kDa in FII compared with 12 bands associated with whole worm extract. Both fractions were also characterized by isoelectric focusing technique which proved that both bands were acidic in nature with pIs 6.4 and 6.5 respectively. The comparative diagnostic evaluation of the two isolated glycoprotein fractions and crude extract of experimental fasciolosis in rabbits by ELISA revealed that FII was more potent in the diagnosis during prepatent (first week post infection) and patent periods (10 weeks post infection) than FI and crude extract. Moreover, infected rabbit sera at ten weeks post infection identified both bands; 26 and 19.5 kDa in western blot analysis confirming its immunodiagnostic activities which was proved previously by ELISA. FII proved potency in diagnosis of fasciolosis in 200 buffalo serum samples of different ages and sexes using ELISA which recorded 95 % positive and 5 % negative samples. Moreover, the detailed structural analyses of the most potent fraction, F11, using mass spectrum was made and elucidated chemical structure; O-glycan [Ser-(Arg-Ser-Arg-Ser-GlucNAc) 19 -GlucNAc]. The present result introduces GlucNAc rich fraction of F .gigantica that can be used successfully in the diagnosis of acute and chronic fasciolosis.

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Phenotypic description and prevalence of Fasciola species in Qena Governorate, Egypt with special reference to a new strain of Fasciola hepatica

Abstract: During the present study morphological description was done for the adult flukes and their eggs and information on the prevalence and worm burden per liver were given.

Cited by 6 publications

References 18 publications

Fasciola hepaticainfections in cattle and the freshwater snailGalba truncatulafrom Dakhla Oasis, Egypt

Fasciola hepaticainfections in cattle and the freshwater snailGalba truncatulafrom Dakhla Oasis, Egypt

Genetically Confirmed Fasciola Hepatogigantica N.SP.

The role of Ser-(Arg-Ser-Arg-Ser-GlucNAc)19-GlucNAc Fasciola gigantica glycoprotein in the diagnosis of prepatent fasciolosis in rabbits

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