Phenotypic comparison between mesothelial and microvascular endothelial cell lineages using conventional endothelial cell markers, cytoskeletal protein markers and in vitro assays of angiogenic potential

Chung‐Welch, Nancy; Patton, Wayne F.; Yen-Patton, G.P. Ameia; Hechtman, Herbert B.; Shepro, David

doi:10.1111/j.1432-0436.1989.tb00606.x

Cited by 82 publications

(36 citation statements)

References 53 publications

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“…The cobblestone appearance of confluent endothelial cells is not unique to endothelial cells but is also a characteristic of cultured mesothelial cells (8). ChungWelch and colleagues reported that endothelial cells could be distinguished from mesothelial cells, which do not have the ability to form capillary-like tube structures when plated onto a Matrigel (34). The uptake of acetylated LDL has been used to distinguish endothelial cells from fibroblasts, smooth muscle cells, and pericytes (35).…”

Section: Discussionmentioning

confidence: 99%

Human Primary Lung Endothelial Cells in Culture

Comhair¹,

Xu²,

Mavrakis³

et al. 2012

Am J Respir Cell Mol Biol

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Pulmonary endothelial functions are critical to maintain the low pressure of the pulmonary circulation and effective diffusion capacity of the lung. To investigate pulmonary endothelial cell biology in healthy or diseased lungs, we developed methods to harvest and culture pure populations of primary pulmonary arterial endothelial cells and microvascular endothelial cells from human lung explanted at time of transplantation or from donor lungs not used in transplantation. The purity and characteristics of cultured endothelial cells is ascertained by morphologic criteria using phase contrast and electron microscopy; phenotypic expression profile for endothelial specific proteins such as endothelial nitric oxide synthase, platelet/endothelial cell adhesion molecule, and von Willbrand factor; and endothelial function assays such as Dilacetylated low-density lipoprotein uptake and tube formation. This detailed method provides researchers with the ability to establish cells for molecular, genetic, and biochemical investigation of human pulmonary vascular diseases.

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Section: Discussionmentioning

confidence: 99%

Human Primary Lung Endothelial Cells in Culture

Comhair¹,

Xu²,

Mavrakis³

et al. 2012

Am J Respir Cell Mol Biol

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“…Most importantly, the HMVECs formed capillary-like structures when seeded on Matrigel (Collaborative Research)-coated tissue culture plates. This characteristic alone uniquely identifies endothelial cells and thus distinguishes microvascular endothelial cells from mesothelial cells (15).…”

Section: Methodsmentioning

confidence: 99%

Human microvascular endothelial cells express receptors for platelet-derived growth factor.

Beitz

Kim

Calabresi

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

126

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“…The vascular endothelium is a heterogeneous group of cells, which differ functionally, not only between large and small blood vessels, but also between different organs and species [1,2]. Unequivocal markers for endothelial cells are difficult to obtain due to this heterogeneity.…”

Section: Introductionmentioning

confidence: 99%