Phenotypic characterization of Sodalis praecaptivus sp. nov., a close non-insect-associated member of the Sodalis-allied lineage of insect endosymbionts

Chari, Abhishek; Oakeson, Kelly F.; Enomoto, Shinichiro; Jackson, D. Grant; Fisher, Mark A.; Dale, Colin

doi:10.1099/ijs.0.000091

Cited by 49 publications

(55 citation statements)

References 20 publications

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“…Inclusion of additional galling psyllids in the future might shed more light on whether this ecological niche promoted a tighter association between hosts and Sendosymbionts. The Sodalis endosymbiont from C. maniformis 1 also exhibited an AT-bias, based on 23S rDNA, when compared to free-living S. praecaptivus (Chari et al, 2015). Interestingly, it was also more AT-biased than two Sodalis lineages that are in a transitional phase from facultative to obligate endosymbionts in the hosts G. morsitans and S. oryzae (Oakeson et al, 2014).…”

Section: Nucleotide Content and Evolutionary Rates Test Of S-endosymbmentioning

confidence: 98%

Codivergence of the primary bacterial endosymbiont of psyllids versus host switches and replacement of their secondary bacterial endosymbionts

Hall

Morrow

Fromont

et al. 2016

Environmental Microbiology

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Coevolution between insects and bacterial endosymbionts contributes to the success of many insect lineages. For the first time, we tested for phylogenetic codivergence across multiple taxonomic scales, from within genera to superfamily between 36 psyllid species of seven recognised families (Hemiptera: Psylloidea), their exclusive primary endosymbiont Carsonella and more diverse secondary endosymbionts (S-endosymbionts). Within Aphalaridae, we found that Carsonella and S-endosymbionts were fixed in one Glycaspis and 12 Cardiaspina populations. The dominant S-endosymbiont was Arsenophonus, while Sodalis was detected in one Cardiaspina species. We demonstrated vertical transmission for Carsonella and Arsenophonus in three Cardiaspina species. We found strong support for strict cospeciation and validated the informative content of Carsonella as extended host genome for inference of psyllid relationships. However, S-endosymbiont and host phylogenies were incongruent, and displayed signs of host switching and endosymbiont replacement. The high incidence of Arsenophonus in psyllids and other plant sap-feeding Hemiptera may be due to repeated host switching within this group. In two psyllid lineages, Arsenophonus and Sodalis genes exhibited accelerated evolutionary rates and AT-biases characteristic of long-term host associations. Together with strict vertical transmission and 100% prevalence within host populations, our results suggest an obligate, and not facultative, symbiosis between psyllids and some S-endosymbionts.

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Section: Nucleotide Content and Evolutionary Rates Test Of S-endosymbmentioning

confidence: 98%

Codivergence of the primary bacterial endosymbiont of psyllids versus host switches and replacement of their secondary bacterial endosymbionts

Hall

Morrow

Fromont

et al. 2016

Environmental Microbiology

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“…[Corrections added on November 23, 2016, after first online publication: Reference citations were corrected in Table 3: row 2, column 7, “Filamentous” was changed to “Filamentous”; row 2, column 8, “[…](Hemiptera: Aphididae)” was changed to “[…](Hemiptera: Aphididae)”. ]…”

Section: Stage 3: Obligate Intracellular Mutualist (Advanced Stage)—nmentioning

confidence: 99%

Dissecting genome reduction and trait loss in insect endosymbionts

Latorre

Manzano-Marı́n

2016

Annals of the New York Academy of Sciences

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Symbiosis has played a major role in eukaryotic evolution beyond the origin of the eukaryotic cell. Thus, organisms across the tree of life are associated with diverse microbial partners, conferring to the host new adaptive traits that enable it to explore new niches. This is the case for insects thriving on unbalanced diets, which harbor mutualistic intracellular microorganisms, mostly bacteria that supply them with the required nutrients. As a consequence of the lifestyle change, from free-living to host-associated mutualist, a bacterium undergoes many structural and metabolic changes, of which genome shrinkage is the most dramatic. The trend toward genome size reduction in endosymbiotic bacteria is associated with large-scale gene loss, reflecting the lack of an effective selection mechanism to maintain genes that are rendered superfluous by the constant and rich environment provided by the host. This genome-reduction syndrome is so strong that it has generated the smallest bacterial genomes found to date, whose gene contents are so limited that their status as cellular entities is questionable. The recent availability of data on several endosymbiotic bacteria is enabling us to form a comprehensive picture of the genome-reduction process and the phenotypic consequences for the dwindling symbiont.

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“…Sequences were assembled and analyzed with Ripseq (Isentio, Palo Alto, CA) and verified by using the GenBank nr database. Phylogenetic analysis of our patient's isolate based on 16S rRNA gene sequences (2,3) clearly shows that it belongs to the W. chitiniclastica clade and exhibits a much closer relationship to other Wohlfahrtiimonas and Ignatzschineria sp. type strains than either A. lwoffii or B. diminuta (Fig.…”

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confidence: 99%

First Report of Wohlfahrtiimonaschitiniclastica Isolation from a Patient with Cellulitis in the United States

et al. 2015

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We report the first documented isolation of Wohlfahrtiimonas chitiniclastica from a human in the United States. Initially misidentified as Acinetobacter lwoffii by Vitek-2, the isolate was subsequently identified as W. chitiniclastica by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing. While the clinical significance of the isolate in this case is unclear, it highlights the superior performance of MALDI-TOF MS for bacterial identification. CASE REPORTA 26-year-old morbidly obese male courier worker was admitted with worsening right leg swelling and draining ulcers. He had developed lymphedema of the right leg during the preceding year after undergoing surgical correction of a right lower extremity varus deformity. A few weeks prior to the current admission, he had noticed right leg ulcers draining yellowish green serosanguineous fluid. On admission, he was afebrile and his white blood cell count was 11,100/l. He was found to have right lower extremity cellulitis.A swab was collected from the right leg and sent to the microbiology laboratory for wound cultures. The sample was inoculated onto blood, chocolate, and MacConkey agars and incubated under aerobic conditions at 37°C. Gram staining revealed many Gram-negative bacilli and moderate Gram-positive cocci. Four different organisms were isolated from the swab after growth. Microbial identification of each isolate was performed by using routine biochemical tests, including automated identification by the Vitek 2 system (bioMérieux, Marcy l'Etoile, France). The organisms were identified as Proteus vulgaris, Klebsiella pneumoniae, Acinetobacter lwoffii, and Staphylococcus aureus. The isolates were subsequently analyzed in triplicate by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) (RUO version 3.1, Bruker Biotyper; Bruker Daltonics, Bremen, Germany). Of note, the isolate identified as A. lwoffii by the Vitek 2 system was identified by MALDI-TOF MS as Wohlfahrtiimonas chitiniclastica, with identification log (score) values of 2.253, 2.296, and 2.229. Log (score)

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Phenotypic characterization of Sodalis praecaptivus sp. nov., a close non-insect-associated member of the Sodalis-allied lineage of insect endosymbionts

Cited by 49 publications

References 20 publications

Codivergence of the primary bacterial endosymbiont of psyllids versus host switches and replacement of their secondary bacterial endosymbionts

Codivergence of the primary bacterial endosymbiont of psyllids versus host switches and replacement of their secondary bacterial endosymbionts

Dissecting genome reduction and trait loss in insect endosymbionts

First Report of Wohlfahrtiimonaschitiniclastica Isolation from a Patient with Cellulitis in the United States

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