Phenotypic characterization of rhizobia isolated from chickpea (Cicer arietinum) growing in Moroccan soils

Abstract

Phenotypic characteristics of fifty-six rhizobia strains isolated from root nodules of two chickpea (Cicer arietinum L.) cultivars, growing in soils collected from different areas of Morocco, were studied. Tolerance to salinity, high temperatures, acid and alkaline pHs, heavy metals and to antibiotics as well as symbiotic and cultural characteristics allowed the description of a wide physiological diversity among tested isolates. Numerical analysis of the phenotypic characteristics showed that, below the boundary level of 82% average similarity, isolates fell into at least five distinct groups. A number of potential isolates have been identified for inoculation trials. They were effective and able to grow at pH ranging from 5 to 8, tolerated salt concentration (1-1.5%) and grew at a maximum temperature between 30 and 35 o C. biodiversity / Cicer arietinum / phenotypic characterization / Rhizobium Résumé-Caractérisation phénotypique des rhizobia isolés du pois chiche (Cicer arietinum) cultivé sur les sols marocains. Cinquante six souches de rhizobium nodulant deux cultivars de pois chiche (Cicer arietinum L.) et isolées de différentes régions du Maroc, ont été caractérisées sur le plan phénotypique. L'analyse de leur tolérance à la salinité, aux températures élevées, aux pH acides et alcalins, aux antibiotiques, aux métaux lourds ainsi que leurs caractéristiques symbiotiques et culturales ont permis de mettre en évidence une large diversité physiologique au sein de ces populations de rhizobium nodulant le pois chiche. L'analyse numérique de ces caractéristiques phénotypiques a montré qu'à un niveau de 82 % de similarité, ces souches bactériennes se sont réparties en cinq groupes distincts. Des souches intéressantes pour les essais d'inoculation ont été identifiées. Elles sont efficientes et capables de tolérer des pHs allant de 5 à 8, des concentrations en NaCl de 1 à 1,5 % et des températures maximales comprises entre 30 à 35 o C. biodiversité / caractérisation phénotypique / pois chiche / Rhizobium Communicated by Jean-Jacques Drevon (Montpellier, France)

Symbiotic and Phenotypic Characteristics of Rhizobia Nodulaing Cowpea (Vigna Unguiculata L. Walp) Grown in Arid Region of Libya (Fezzan)

“…Monosaccharides and sugar alcohols (D-glucose, D-fructose, galactose, sorbitol and inositol) were poorly utilized by most test isolates, whereas, disaccharides (sucrose and maltose) were assimilated by most of them. Thus, they are in line with what reported by others for chickpea rhizobia [47]. Few isolates in different groups were used starch as a carbon source, but with exception of separate isolates and one isolate in group 3, all other groups were failed to use cellulose.…”

Phenotypic characterization and symbiotic effectiveness test of chickpea (Cicer arietinum L.) rhizobia isolated from Dejen and Aneded Districts, East Gojjam Zone, Amahara Region, Ethiopia

“…Isolates grown on the Streptomycin showed very less tolerance with respect to concentration. Several studies reported the existence of broad variation among chickpea rhizobia with respect to the fate of their intrinsic antibiotics resistance (Maâtallah et al, 2002;Kucuk and Kıvanc, 2008). The isolates' sensitivity to antibiotics may be due to inability to resist exposed toxicity with less adaptation in natural environments (Singh and Bamania, 2012).…”

Characterization of Phosphate Solubilizing Faba Bean (<i>Vicia faba</i> L.) Nodulating Rhizobia Isolated from Acidic Soils of Wollega, Ethiopia

“…Tolerance of the isolates to extreme temperature, pH, and salt was checked according to Maatallah et al (2002). Intrinsic antibiotics resistance (IAR) of isolates to Kanamycin sulfate, Streptomycin sulfate, Chloramphenicol, Ampicillin, Neomycin sulfate and Erythromycin with concentration of (μg/ml ) 2.5, 5, and 10 was tested.…”