Phenotypic characterization of human keratinocytes in coculture reveals differential effects of fibroblasts from benign fibrous histiocytoma (dermatofibroma) as compared to cells from its malignant form and to normal fibroblasts

Kideryová, L; Lacina, Lukáš; Dvořánková, Barbora; Štork, Jiří; Čada, Zdeněk; Szabó, Pavol; André, Sabine; Kaltner, Herbert; Gabius, Hans‐Joachim; Smetana, Karel

doi:10.1016/j.jdermsci.2009.03.009

Cited by 5 publications

(5 citation statements)

References 42 publications

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“…It was previously reported that fibroblasts can affect the phenotypic characterisation of keratinocytes in co-culture 22 23. However, epithelial cell sheets cultivated in the current study did not express K1 or K10, markers for suprabasal cells in the epidermis.…”

Section: Discussioncontrasting

confidence: 70%

A novel method of culturing human oral mucosal epithelial cell sheet using post-mitotic human dermal fibroblast feeder cells and modified keratinocyte culture medium for ocular surface reconstruction

Oie

Hayashi²,

Takagi³

et al. 2010

British Journal of Ophthalmology

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Section: Discussioncontrasting

confidence: 70%

A novel method of culturing human oral mucosal epithelial cell sheet using post-mitotic human dermal fibroblast feeder cells and modified keratinocyte culture medium for ocular surface reconstruction

Oie

Hayashi²,

Takagi³

et al. 2010

British Journal of Ophthalmology

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“…Furthermore, these small keratinocytes were observed earlier in fetal/neonatal epidermis of human and porcine origin, respectively ( 6 , 32 ). Fibroblasts isolated from epidermal carcinomas and dermatofibroma revealed a similar effect to AKs in the co-culture ( 17 , 19 , 33 ). Similarly, melanoma cells and neural crest stem cells isolated from hair follicles induced the presence of small cells at the periphery of AK colonies ( 34 ).…”

Section: Discussionmentioning

confidence: 78%

Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro

Mateu

Živicová

Krejčí

et al. 2016

International Journal of Molecular Medicine

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Clinical evidence suggests that healing is faster and almost scarless at an early neonatal age in comparison with that in adults. In this study, the phenotypes of neonatal and adult dermal fibroblasts and keratinocytes (nestin, smooth muscle actin, keratin types 8, 14 and 19, and fibronectin) were compared. Furthermore, functional assays (proliferation, migration, scratch wound closure) including mutual epithelial-mesenchymal interactions were also performed to complete the series of experiments. Positivity for nestin and α smooth muscle actin was higher in neonatal fibroblasts (NFs) when compared with their adult counterparts (adult fibroblasts; AFs). Although the proliferation of NFs and AFs was similar, they significantly differed in their migration potential. The keratinocyte experiments revealed small, poorly differentiated cells (positive for keratins 8, 14 and 19) in primary cultures isolated from neonatal tissues. Moreover, the neonatal keratinocytes exhibited significantly faster rates of healing the experimentally induced in vitro defects in comparison with adult cells. Notably, the epithelial/mesenchymal interaction studies showed that NFs in co-culture with adult keratinocytes significantly stimulated the adult epithelial cells to acquire the phenotype of small, non-confluent cells expressing markers of poor differentiation. These results indicate the important differences between neonatal and adult cells that may be associated with improved wound healing during the early neonatal period.

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“…However, the opposite effect of these cells was also noted, for example in cancer of pancreas [ 88 ]. We demonstrated that fibroblasts isolated from basal cell carcinoma of the skin, squamous cell carcinoma of the head and neck and dermatofibroma of the skin stimulate expression of keratins 8 and 19 as well as of marker of mesenchymal cells—vimentin in cocultured keratinocytes [ 89 , 90 , 91 ]. This co-expression of keratins and vimentin and expression of transcription factor Snail in nuclei of remarkable proportion of keratinocytes under the influence of CAFs indicate that keratinocytes are in epithelial–mesenchymal transition, process so important for cancer cell metastases formation [ 92 ].…”

Section: Cafs As the Master Cell Type In Tumor Stromamentioning

confidence: 99%

Cancer Microenvironment: What Can We Learn from the Stem Cell Niche

Lacina

Plzák

Kodet

et al. 2015

IJMS

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Epidermal stem cells (ESCs) are crucial for maintenance and self- renewal of skin epithelium and also for regular hair cycling. Their role in wound healing is also indispensable. ESCs reside in a defined outer root sheath portion of hair follicle—also known as the bulge region. ECS are also found between basal cells of the interfollicular epidermis or mucous membranes. The non-epithelial elements such as mesenchymal stem cell-like elements of dermis or surrounding adipose tissue can also contribute to this niche formation. Cancer stem cells (CSCs) participate in formation of common epithelial malignant diseases such as basal cell or squamous cell carcinoma. In this review article, we focus on the role of cancer microenvironment with emphasis on the effect of cancer-associated fibroblasts (CAFs). This model reflects various biological aspects of interaction between cancer cell and CAFs with multiple parallels to interaction of normal epidermal stem cells and their niche. The complexity of intercellular interactions within tumor stroma is depicted on example of malignant melanoma, where keratinocytes also contribute the microenvironmental landscape during early phase of tumor progression. Interactions seen in normal bulge region can therefore be an important source of information for proper understanding to melanoma. The therapeutic consequences of targeting of microenvironment in anticancer therapy and for improved wound healing are included to article.

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Phenotypic characterization of human keratinocytes in coculture reveals differential effects of fibroblasts from benign fibrous histiocytoma (dermatofibroma) as compared to cells from its malignant form and to normal fibroblasts

Cited by 5 publications

References 42 publications

A novel method of culturing human oral mucosal epithelial cell sheet using post-mitotic human dermal fibroblast feeder cells and modified keratinocyte culture medium for ocular surface reconstruction

A novel method of culturing human oral mucosal epithelial cell sheet using post-mitotic human dermal fibroblast feeder cells and modified keratinocyte culture medium for ocular surface reconstruction

Functional differences between neonatal and adult fibroblasts and keratinocytes: Donor age affects epithelial-mesenchymal crosstalk in vitro

Cancer Microenvironment: What Can We Learn from the Stem Cell Niche

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