Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis

Santana, Jordana Batista; Almeida, Tarcísio Vila Verde Santana de; Lopes, Diego Mota; Page, Brady; Oliveira, Sérgio C.; Souza, Irismá; Ribeiro, Luis Eduardo; Gutiérrez, Néstor Adrián Guerrero; Carvalho, Edgar M.; Cardoso, Luciana Santos

doi:10.3389/fimmu.2021.605235

Cited by 6 publications

(5 citation statements)

References 44 publications

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“…It is known that T lymphocytes are essential for immune responses against Schistosoma species. 31 In the case of schistosomiasis, the granulomatous inflammation and the liver pathology are critically regulated by the Th1, Th2, Th17, and regulatory T (Treg) responses. 32,33 We detected changes in T-lymphocyte immune regulation in the spleen of mice injected with Sj16-KO eggs by flow cytometry.…”

Section: Knockout Of Sj16 In S Japonicum Eggs Increased T-lymphocyte ...mentioning

confidence: 99%

CRISPR/Cas9‐mediated gene knockout of Sj16 in Schistosoma japonicum eggs upregulates the host‐to‐egg immune response

et al. 2022

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Schistosomiasis is an important, neglected tropical disease. Schistosoma japonicum can evade host attacks by regulating the host's immunity, causing continuous infection. However, interactions between the host's immune system and S. japonicum are unclear. Our previous research found that the Sj16 protein isolated from S. japonicum has an anti-inflammatory effect in the host. However, the role of Sj16 in the regulation of host immunity in S. japonicum infection is not clear.Here, we applied the CRISPR/Cas9 technique to knockout Sj16 in S. japonicum eggs and investigated the effect of Sj16 in regulating host immunity. We found egg viability decreased after Sj16 knockout. In addition, we found granulomatous inflammation increased, the T-cell immune response enhanced and the immune microenvironment changed in mice model injected with Sj16-knockout eggs by tail vein. These findings suggested that S. japonicum could regulate host immunity through Sj16 to evade the host immune attack and cause continuous infection. In addition, we confirmed the application of CRISPR/Cas9-mediated gene reprogramming for functional genomics in S. japonicum.

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Section: Knockout Of Sj16 In S Japonicum Eggs Increased T-lymphocyte ...mentioning

confidence: 99%

CRISPR/Cas9‐mediated gene knockout of Sj16 in Schistosoma japonicum eggs upregulates the host‐to‐egg immune response

et al. 2022

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“…Schistosome infection leads to significant changes in immune function in patients, with the Th1 response dominating in the acute phase, but gradually shifting to a Th2 response as the schistosomes mature over time 16,18,22,37 . And it in turn gradually diminishes with the formation of chronic granulomas 16,22 .…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Peripheral Lymphocyte Subsets in Patients with Different Stages of Schistosomiasis Japonica

Ming

Zhou

et al. 2023

Preprint

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Background Immune cells are important for the development of schistosomiasis japonica and are also critical for the treatment of schistosomiasis. The immune cells in the peripheral blood help assess the immune state. The peripheral lymphocytes in schistosomiasis mansoni were well studied, however immune cells in patients with different stages of Schistosomiasis Japonica are not well analyzed. Here we performed a preliminary study to explore characteristics of peripheral lymphocyte subsets in patients with different stages of Schistosomiasis Japonica. Methods 135 patients with S. japonicum infection and 25 healthy volunteers were included in this study, including 84 patients with chronic S. japonicum infection and 51 patients with advanced S. japonicum infection. Flow cytometry analysis was performed to evaluate peripheral lymphocytes including T cells, B cells and NK cells. Blood routine and liver function test data were analyzed. Ultrasound examination was used to access liver fibrosis according to the World Health Organization standard about ultrasound in schistosomiasis. Results Demographic data analysis suggested there was no difference in age and gender in patients with S. japonicum infection and health control group. Liver function tests showed that patients with advanced schistosomiasis had a higher incidence of liver function abnormality and blood lipid than those with chronic schistosomiasis. Blood routine results reflected that hemoglobin, red blood cells, platelets, as well as lymphocytes in the advanced group were significantly less than that in the chronic group. Furthermore, flow cytometry analysis indicated that the percentage of CD4 T cells was lower in the advanced group, but the percentage of CD19 B cells was higher in the advanced group. In addition, the number of CD3 T cells, CD3 CD4 T cells, CD3 CD8 T cells, and NK cells was less in the advanced group when compared with those in the chronic group. In addition, there was a correlation between the decrease in CD4 T cells and more severe fibrosis on ultrasound images. Conclusion Our results indicated that the immune state in the peripheral is different in different stages of S. japonicum infection. Lymphocyte subset analysis have potential to facilitate differential diagnosis of different stages of schistosomiasis japonica and even to be a prognostic factor.

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“…Schistosome infection leads to significant changes in immune function in patients, with the Th1 response dominating in the acute phase, but gradually shifting to a Th2 response as the schistosomes mature over time. 21,23,27,42 And, it in turn gradually diminishes with the formation of chronic granulomas. 21,27 Cytokines have received more attention in many studies, but direct immune cells have been less frequently mentioned.…”

Section: Comparison Of Peripheral Lymphocyte Subsets Between Patients...mentioning

confidence: 99%

Characteristics of peripheral lymphocyte subsets in patients with different stages of schistosomiasis japonica

Zhou

Jiang

et al. 2023

Parasite Immunology

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Immune cells are important for the development of schistosomiasis japonica and are also critical for the treatment of schistosomiasis. The immune cells in the peripheral blood help assess the immune state. The peripheral lymphocytes in schistosomiasis mansoni were well studied; however, immune cells in patients with different stages of schistosomiasis japonica are not well analysed. Here, we performed a preliminary study to explore characteristics of peripheral lymphocyte subsets in patients with different stages of schistosomiasis japonica. 135 patients with Schistosoma japonicum infection and 25 healthy volunteers were included in this study, including 84 patients with chronic S. japonicum infection and 51 patients with advanced S. japonicum infection. Flow cytometry analysis was performed to evaluate peripheral lymphocytes including T cells, B cells, and natural killer (NK) cells. Blood routine and liver function test data were analysed. Ultrasound examination was used to access liver fibrosis according to the World Health Organization standard about ultrasound in schistosomiasis. Demographic data analysis suggested there was no difference in age and gender in patients with S. japonicum infection and health control group. Liver function tests showed that patients with advanced schistosomiasis had a higher incidence of liver function abnormality and blood lipid than those with chronic schistosomiasis. Blood routine results reflected that haemoglobin, red blood cells, platelets, as well as lymphocytes in the advanced group were significantly less than that in the chronic group. Furthermore, flow cytometry analysis indicated that the percentage of CD4+ T cells was lower in the advanced group, but the percentage of CD19+ B cells was higher in the advanced group. In addition, the number of CD3+ T cells, CD3+ CD4+ T cells, CD3+ CD8+ T cells, and NK cells was less in the advanced group when compared with those in the chronic group. In addition, there was a correlation between the decrease in CD4+ T cells and more severe fibrosis on ultrasound images. Our results indicated that the immune state in the peripheral is different in different stages of S. japonicum infection. Lymphocyte subset analysis has potential to facilitate differential diagnosis of different stages of schistosomiasis japonica and even to be a prognostic factor.

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Phenotypic Characterization of CD4+ T Lymphocytes in Periportal Fibrosis Secondary to Schistosomiasis

Cited by 6 publications

References 44 publications

CRISPR/Cas9‐mediated gene knockout of Sj16 in Schistosoma japonicum eggs upregulates the host‐to‐egg immune response

CRISPR/Cas9‐mediated gene knockout of Sj16 in Schistosoma japonicum eggs upregulates the host‐to‐egg immune response

Characteristics of Peripheral Lymphocyte Subsets in Patients with Different Stages of Schistosomiasis Japonica

Characteristics of peripheral lymphocyte subsets in patients with different stages of schistosomiasis japonica

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