Phenotypic characterization of auxotrophic mutant of nontyphoidal Salmonella and determination of its cytotoxicity, tumor inhibiting cytokine gene expression in cell line models
Abstract:An auxotrophic mutant of non-typhoidal Salmonella (NTS) strain was phenotypically characterized in this study. The characterization was based on phenotype, morphology, motility, bio lm forming ability, growth kinetics, etc. The phenotypic results from the above experiments determined that the mutant showed variation in phenotypic characters from that of wild-type strain. Subsequently, mutant and wild-type NTS were subjected to epithelial cell invasion and intracellular replication assays. The real time PCR ana… Show more
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