Phenotypic Characterization of Aeromonas hydrophila Isolates in Fresh Water Fishes in FCT Using MicrobactTM GNB 24E Identification Kit

Mailafia, S; Nabilah, B.; Olabode, Olatunde Hamza

doi:10.4236/oalib.1107066

Cited by 9 publications

(9 citation statements)

References 8 publications

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“…These organisms are able to grow on sheep blood agar and produce β hemolysis. According to the product information from the manufacturer, the Microbact TM 24E system (Oxoid) can identify the following species, A. hydrophila, A. veronii bv sobria, A. veronii bvveronii, and A. caviae [26].…”

Section: Etiologymentioning

confidence: 99%

A review on aeromoniasis in poultry: A bacterial disease of zoonotic nature

El-Ghany

2023

J Infect Dev Ctries

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Aeromonas spp. are widely distributed in surface water, sewage, untreated and chlorinated, drinking water, as well as meats, fish, shellfish, poultry, and their products. A disease caused by Aeromonas spp. is designated as aeromoniasis. It can affect different aquatic animals, mammals, and birds in different geographic regions. Moreover, gastrointestinal and extra-intestinal disease conditions may be provoked in humans as a result of food poising with Aeromonas spp. Some Aeromonas spp. have been identified, however, Aeromonas hydrophila (A. hydrophila), A. caviae, and A. veronii bv sobria may be of public health significance. Aeromonas spp. are members of family Aeromonadaceae and genus Aeromonas. They are Gram-negative rod-shaped, facultative anaerobic, and oxidase and catalase-positive bacteria. The pathogenicity of Aeromonas in different hosts is mediated by several virulence factors such as endotoxins, cytotoxic enterotoxin, cytotoxins, hemolysins, adhesins, and extracellular enzymes such as proteases, amylases, lipases, ADP-ribosyltransferases, and DNases. Most avian species are susceptible to either natural or experimental infections with Aeromonas spp. Infection usually arises through feacal-oral route. Traveler’s diarrhea as well as other systemic and local infections are the clinical picture of food poisoning associated with aeromoniasis in humans. Despite Aeromonas spp. being sensitive to various antimicrobials, multiple drug resistance has been commonly reported worldwide. Accordingly, this review highlights aeromoniasis in poultry regarding Aeromonas virulence factors epidemiology, pathogenicity, zoonosis, and antimicrobial resistance.

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Section: Etiologymentioning

confidence: 99%

A review on aeromoniasis in poultry: A bacterial disease of zoonotic nature

El-Ghany

2023

J Infect Dev Ctries

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“…The plates were incubated at 37 ± 2 ˚C for 48 hours. After 48 hours, the adhesive seal was peeled and Nitrate, Kovacs, Voges -Proskauer reaction (VP) and tryptophan deaminase (TDA) reagents were added to wells 7, 8, 10 and 12 respectively and colour changes of the different tests were observed and recorded in the booklet which was transcribed into an 8 digit code and organisms were interpreted as stipulated by the manufacturers using the microbact software version Microbact TM 200 identification package V2.03 (Windows TM ) which is a computer identification software which immediately gave the probable identity of the Aeromonas species (Mailafia et al, 2021).…”

Section: Biochemical Identification Of Aeromonas Speciesmentioning

confidence: 99%

“…Disease at any stage of the fish culture is a significant setback that usually leads to great consequences on the economic viability of fish farms and on the yield of protein for human consumption (Tavares-Dias & Martins, 2017;Elsheshtawy, et al, 2019). Gram-negative bacteria like Aeromonas species are the most common causes of morbidity and mortality leading to great economic losses in the aquaculture industry (Mailafia et al, 2021;Pepi & Focardi, 2021). Aeromonas species are ubiquitous bacteria which are commonly isolated from freshwater ponds and different holding facilities (Adah et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

2023

JoSVAS

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Aeromonas species are associated with diseases on fish farms leading to mortality and economic losses. In order to determine the prevalence and diversity of Aeromonas species from water and Clarias gariepinus cultured in the different holding facilities; five hundred and seventy six Clarias gariepinus fish and one hundred and eight water samples were randomly sampled from fish farms in Kwara State. The samples were clinically and bacteriologically examined and confirmed using Oxiod rapid microbat identification test kits for Gram-negative bacteria, Microbact 24E (MB24E). The overall prevalence of Aeromonas species from water from the different cultured facilities revealed water sampled from earthen ponds had the highest prevalence of 66.67 % followed by concrete tanks with a prevalence of 41.66 % and the least prevalence of 30.56 % was recorded for water in plastic tanks. A similar pattern was recorded in Clarias gariepinus from the different holding facilities showing that earthen ponds had the highest prevalence of 40.10 % when compared with concrete tanks 28.65%, and to plastic tanks 20.83 %. Four varying diversities of Aeromonas species comprising of Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii biovar sobria, and Aeromonas veronii biovar veronii were isolated from both water and fish from the different holding facilities. From this study, cultured Clarias gariepinus were susceptible to Aeromonas species irrespective of the culture facilities. Therefore, there is need for proper management practices and adherence to biosecurity measures to prevent the outbreak and spread of diseases on the farms.

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“…Fish are subject to a wide range of infectious diseases brought on by parasites, bacteria, fungi, viruses, and other organisms that use them as a rich supply of nutrients. Bacteria are known to cause the greatest number of fatalities of any pathogenic agent, both in the wild and in culture [9]. Bacterial infection of fish constitutes a huge menace for aquaculture, leading to disastrous economic loss [10].…”

Section: Introductionmentioning

confidence: 99%

Study the optimal conditions of Aeromonas and Vibrio species isolated from Cyprinus carpio of some farms in Babil, Iraq.

basim hameed,

kadhim Emran,

Al-Khalaf

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Four environmental bacterial isolates, including Aeromonas hydrophila, Aremonas soberia, Aremonas veronii, and Vibirio cholerae, were isolated from living Carp in some farms in Babylon, Iraq, and examined for their responses to temperature, pH, and incubation period under laboratory conditions in this study. In addition, bacterial growth under test conditions was measured to obtain important parameters related to bacterial growth, lag time (λ), specific growth rate (μm), and maximum biomass (A) for comparison. V. cholerae did show the lowest mean growth at different temperatures, but was adapted to a much wider environmental pH from 4 to 9 for growth, while A. hydrophila was more adaptable to pH and temperature changes, yielding the highest mean biomass at pH 8 and temperature 40 °C. A. sobria was surprisingly tolerant to different incubation periods and grew almost equally well under the conditions of other treatments. All four bacterial isolates showed a wide spectrum of plasticity to environmental conditions, and they pose a potential threat to public health and animal health.

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Phenotypic Characterization of Aeromonas hydrophila Isolates in Fresh Water Fishes in FCT Using MicrobactTM GNB 24E Identification Kit

Cited by 9 publications

References 8 publications

A review on aeromoniasis in poultry: A bacterial disease of zoonotic nature

A review on aeromoniasis in poultry: A bacterial disease of zoonotic nature

Prevalence of Aeromonas species in Clarias gariepinus and water from different culture facilities in fish farms in Kwara State, Nigeria

Study the optimal conditions of Aeromonas and Vibrio species isolated from Cyprinus carpio of some farms in Babil, Iraq.

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