Phenotypic characterization in natural population of Spot Blotch (Bipolaris sorokiniana) of Barley (Hordeum vulgare L.)

Abstract

A study was undertaken on phenotypic characterization of different isolates of Bipolaris sorokiniana isolated from the different varietal trials and nurseries of barley at the agricultural research farm, Institute of Agricultural sciences, Banaras Hindu University. The isolation was performed by transfer of single spore of the pathogen from diseased leaf in Potato Dextrose Agar media (PDA) under aseptic condition. The experiment revealed five groups of isolates on the basis of colony colour, morphology and number of spores. It was found that (42.2%) of the isolates were black in colour with profuse sporulation and suppressed type growth, (35.71%) of the isolates were greyish in colour and (21.42%) of the isolates possessed whitish colony with full plate growth and lower sporulation. The size of conidia ranged from 24 × 13µm to 36 × 14µm in size, while the number of spores per mm2 of colony varied from 20.36 to 162.77.