Phenotypic Characterization and Heterogeneity among Modern Clinical Isolates of Acinetobacter baumannii

Valček, Adam; Philippe, Chantal; Whiteway, Clémence; Robino, Etienne; Nešporová, Kristina; Bové, Mona; Coenye, Tom; Pooter, Tim De; Coster, Wouter De; Stražišar, Mojca; Henst, Charles Van der

doi:10.1128/spectrum.03061-22

Cited by 15 publications

(34 citation statements)

References 71 publications

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“…This strain is capsulated, multidrug-resistant, and virulent. 33 Therefore, the strain was also chosen in this study for the further characterization of the Nb-bacterium interaction by fluorescence microscopy. After incubating sulfoCy3-labeled NbD4 with the bacteria, the labeled nanobody allowed screening for its binding to bacterial cells.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…33,62 These 6 clinical isolates have striking differences: they differ in capsule types and thicknesses, different antibiograms, and were isolated from Belgian hospitals in the last 8 years. 33,34 In addition, 2 frequently used lab strains, ATCC19606 and ATCC17978, were included as well. By fluorescence microscopy, the binding ability of peptide 3 was investigated on these A. baumannii strains.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…baumannii strain AB5075-VUB. This strain is capsulated, multidrug-resistant, and virulent . Therefore, the strain was also chosen in this study for the further characterization of the Nb-bacterium interaction by fluorescence microscopy.…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, a high diversity is found in clinical isolates, with a strikingly small core genome of 16.5%. 32,33 Moreover, 25% of the genome is unique in A. baumannii isolates. 34 This represents a challenge in the identification of broadly active compounds recognizing the majority of A. baumannii isolates.…”

Section: ■ Introductionmentioning

confidence: 99%

“…baumannii. Consequently, a high diversity is found in clinical isolates, with a strikingly small core genome of 16.5%. , Moreover, 25% of the genome is unique in A. baumannii isolates .…”

Section: Introductionmentioning

confidence: 99%

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Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria

et al. 2023

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Membrane interaction constitutes to be an essential parameter in the mode of action of entities such as proteins, as well as cell-penetrating and antimicrobial peptides, resulting in noninvasive or lytic activities depending on the membrane compositions and interactions. Recently, a nanobody able to interact with the top priority, multidrug-resistant bacterial pathogen Acinetobacter baumannii was discovered, although binding took place with fixed cells only. To potentially overcome this limitation, linear peptides corresponding to the complementarity-determining regions (CDR) were synthesized and fluorescently labeled. Microscopy data indicated clear membrane interactions of the CDR3 sequence with living A. baumannii cells, indicating both the importance of the CDR3 as part of the parent nanobody paratope and the improved binding ability and thus avoiding the need for permeabilization of the cells. In addition, cyclization of the peptide with an additionally introduced rigidifying 1,2,3-triazole bridge retains its binding ability while proteolytically protecting the peptide. Overall, this study resulted in the discovery of novel peptides binding a multidrug-resistant pathogen.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

“…baumannii. Consequently, a high diversity is found in clinical isolates, with a strikingly small core genome of 16.5%. , Moreover, 25% of the genome is unique in A. baumannii isolates .…”

Section: Introductionmentioning

confidence: 99%

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Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria

et al. 2023

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Innate immune mechanisms promote human response to Acinetobacter baumannii infection

Sabatini,

Lucidi,

Ciolfi

et al. 2024

Eur J Immunol

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Acinetobacter baumannii is an opportunistic Gram‐negative bacterium representing one of the leading causes of ventilator‐associated pneumonia. The development of pneumonia results from a complex interplay between pathogens and pulmonary innate mucosal immunity. Therefore, the knowledge of the host immune responses is pivotal for the development of effective therapeutics to treat A. baumannii infections. Previous studies were conducted using cell lines and animal models, but a comprehensive understanding of the interaction between A. baumannii and primary human immune cells is still lacking. To bridge this gap, we investigated the response of primary monocytes, macrophages, and dendritic cells to the A. baumannii‐type strain and an epidemic clinical isolate. We found that all immune cells trigger different responses when interacting with A. baumannii. In particular, macrophages and monocytes mediate bacterial clearance, whereas monocytes and dendritic cells activate a late response through the production of cytokines, chemokines, and the expression of co‐stimulatory molecules. The epidemic strain induces lower expression of interleukin‐10 and CD80 compared with the type strain, potentially constituting two immune evasion strategies.

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Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy

Coenye,

Ahonen,

Anderson

et al. 2024

Biofilm

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Phenotypic Characterization and Heterogeneity among Modern Clinical Isolates of Acinetobacter baumannii

Abstract: Acinetobacter baumannii is a bacterium prioritized by the CDC and WHO because of its increasing antibiotic resistance, leading to treatment failures. The hallmark of this pathogen is the high heterogeneity observed among isolates, due to a very dynamic genome.

Cited by 15 publications

References 71 publications

Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria

Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria

Innate immune mechanisms promote human response to Acinetobacter baumannii infection

Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy

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