Phenotypic characterization and adhesive properties of vaginal Candida spp. strains provided by the CHU Farhat Hached (Sousse, Tunisia)

Noumi, Emira; Snoussi, Mejdi; Noumi, Inès; Saghrouni, F.; Aouni, Mahjoub; Valentı́n, Eulogio

doi:10.1016/j.riam.2014.06.006

Cited by 6 publications

(11 citation statements)

References 42 publications

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“…Several virulence factors were investigated from the fungal suspensions prepared at approximately 10 7 UFC/ml (according the method of Noumi et al (30). Briefly, 2mL of sterile YEPD broth was inoculated with a young colony obtained on SDA + chloramphenicol agar and incubated at 30°C.…”

Section: Research For Virulence Factorsmentioning

confidence: 99%

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Efficacy of Extracts of Cyanthillium Cinereum, Khaya senegalensis and Lippia multiflora on Candida Strains Isolated From Urine Samples in Benin (West Africa)

Fanou¹,

Klotoé²,

Dougnon³

et al. 2022

Front. Trop. Dis

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The search for new bioactive molecules with antifungal properties to combat resistance to classical antifungals represents a great challenge. This study aimed to explore the virulence factors and resistance profile of Candida species isolated from urine samples in Benin and the in vitro efficacy of organic extracts of Cyanthillium cinereum (L.) H.Rob., Lippia multiflora Moldenke and Khaya senegalensis (Desv.) A.Juss. on the growth of these Candida spp. The study focused on Candida strains isolated from urine samples collected from patients admitted to the bacteriological analysis laboratories of hospitals in Southern Benin. The sensitivity of these strains to classical antifungal agents was determined by the simple diffusion method. Their pathogenicity was investigated via several virulence factors (gelatinase, hemolysin, hydrophobicity, adhesin, biofilm and lecithinase). The in vitro efficacy of the aqueous, ethanolic and hydro-ethanolic extracts of the plants on Candida albicans ATCC 90028 and on six clinical strains was evaluated by the method of determination of the inhibition diameters. The results obtained showed that 51 different Candida strains were isolated from the collected urine samples with a respective predominance of Candida albicans (52.94%) and Candida glabrata (17.64%) species. All identified species were sensitive to amphotericin B and nystatin but 20% are resistant to fluconazole and present 15 different resistance profiles. Six different virulence factors were identified with a high frequency of hydrophobicity (96.08%) and adhesin (94.12%). Antifungal tests revealed that at 100 mg/mL the plant extracts were active on the tested strains with better activity for Cyanthilium cinereum and Khaya senegalensis. Cyanthilium cinereum, Khaya senegalensis and Lippia multiflora showed antifungal activity on virulent Candida strains suggesting the possibility to explore them further for the discovery of new antifungal molecules.

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Section: Research For Virulence Factorsmentioning

confidence: 99%

“…The last wash before staining was performed after the incubation time. The adhesion and biofilm formation powers of the strains were determined from the optical densities read and then interpreted according to the criteria specified by Noumi et al (30).…”

Section: Adhesin and Biofilm Activitiesmentioning

confidence: 99%

Efficacy of Extracts of Cyanthillium Cinereum, Khaya senegalensis and Lippia multiflora on Candida Strains Isolated From Urine Samples in Benin (West Africa)

Fanou¹,

Klotoé²,

Dougnon³

et al. 2022

Front. Trop. Dis

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“…At this level, a cell suspension is prepared according to the modi ed method of Noumi et al [12]. Indeed, it was performed a dilution of the fungal cell concentrate in PBS (centrifugation pellet + 500µL of PBS) and in RPMI 1640 medium to have the density of the suspension obtained adjusted to 0.38 at 520 nm corresponding to 10 7 UFC/ml (50µL of the PBS suspension).…”

Section: Assessment Of Adhesion Strength and Bio Lm Formationmentioning

confidence: 99%

Biological properties and antifungal activity of Acalypha wilkesiana, Mitracarpus hirtus, Senna alata on Candida strains isolated from hospital stool samples in Southern Benin

Klotoé

Fah

Fanou

et al. 2022

Preprint

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Candidiasis is one of the opportunistic diseases that occur following HIV/AIDS infection. Currently, the management of candidiasis is problematic due to the emergence of resistance to classical antifungal drugs. The search for alternative solutions for the discovery of new bioactive molecules is necessary. This study aims to explore the antifungal properties of Mitracarptus hirtus, Acalypha wikesian and Senna alata on Candida strains isolated from stools of people living with the acquired immunodeficiency virus and collected in some hospitals in Benin and then confirmed by molecular biology techniques at the Research Unit in Microbiology and Pharmacology of natural substances. The strains were tested for different resistance factors. The main phytochemical groups of the plants were searched by staining methods from the dry matter of the studied plants. The determination of total polyphenols and the evaluation of the antioxidant activity of the extracts by the DPPH radical scavenging test were carried out by spectrophotometric methods. The in vitro antifungal activity of the aqueous and hydroethanol extracts of these plants was then evaluated on the strains. The antifungal test data revealed that of the three plants tested, only Mitracarptus hirtus showed inhibitory activity on the growth of the reference strain of Candida albicans ATCC 90028. The hydroethanol extract of this plant was active on all clinical strains with a better effect at the concentration of 100 mg/ml. The data obtained also highlighted the richness in total polyphenols and the anti-free radical activity of the plant extracts studied. This study proved particularly the anti-candidus properties of the hydro-ethanolic extract of the leafy stem of Mitracarptus hirtus. Further studies will generate data on the in vivo efficacy and mechanism of action of the anti-candidosis effect of Mitracarptus hirtus.

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“…Other hydrolytic activities like proteinase, hemolytic factors, and DNase have been reported in C. krusei. 60,67,68 A study that included clinical isolates from Turkish patients diagnosed with candidiasis, found that about half of the C. krusei isolates formed biofilms, 22% showed coagulase activity and all the isolates were capable of hemolyzing red blood cells. 69 In C. albicans, most of the secreted proteolytic activity is associated with members of the SAP gene family.…”

Section: Recent Understanding Of the Host-pathogen Interaction Virulementioning

confidence: 99%

“…C. krusei is capable of forming biofilms on polyethylene, polyvinylchloride, and glass. 67,72 These fungal biofilms are particularly sensitive to fluconazole when generated on polystyrene surfaces. 73 This represents a promising observation that remains to be confirmed in vivo.…”

Section: Recent Understanding Of the Host-pathogen Interaction Virulementioning

confidence: 99%

<p>Current Aspects in the Biology, Pathogeny, and Treatment of <em>Candida krusei</em>, a Neglected Fungal Pathogen</p>

Gómez-Gaviria

Mora-Montes

2020

IDR

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Fungal infections represent a constant and growing menace to human health, because of the emergence of new species as causative agents of diseases and the increment of antifungal drug resistance. Candidiasis is one of the most common fungal infections in humans and is associated with a high mortality rate when the fungi infect deep-seated organs. Candida krusei belongs to the group of candidiasis etiological agents, and although it is not isolated as frequently as other Candida species, the infections caused by this organism are of special relevance in the clinical setting because of its intrinsic resistance to fluconazole. Here, we offer a thorough revision of the current literature dealing with this organism and the caused disease, focusing on its biological aspects, the host-fungus interaction, the diagnosis, and the infection treatment. Of particular relevance, we provide the most recent genomic information, including the gene prediction of some putative virulence factors, like proteases, adhesins, regulators of biofilm formation and dimorphism. Moreover, C. krusei veterinary aspects and the exploration of natural products with anti-C. krusei activity are also included.

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Phenotypic characterization and adhesive properties of vaginal Candida spp. strains provided by the CHU Farhat Hached (Sousse, Tunisia)

Cited by 6 publications

References 42 publications

Efficacy of Extracts of Cyanthillium Cinereum, Khaya senegalensis and Lippia multiflora on Candida Strains Isolated From Urine Samples in Benin (West Africa)

Efficacy of Extracts of Cyanthillium Cinereum, Khaya senegalensis and Lippia multiflora on Candida Strains Isolated From Urine Samples in Benin (West Africa)

Biological properties and antifungal activity of Acalypha wilkesiana, Mitracarpus hirtus, Senna alata on Candida strains isolated from hospital stool samples in Southern Benin

<p>Current Aspects in the Biology, Pathogeny, and Treatment of <em>Candida krusei</em>, a Neglected Fungal Pathogen</p>

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