Phenotypic Characterisation of Carbapenemases Produced by Enterobacteria Isolated from Patients of the Medico-Social Centre of the National Social Insurance Fund of Maroua: Cameroon

Abstract

The aim of this study is to determine the types of carbapenemases moving around the city of Maroua with a view to contribute to the development of a control strategy against the enterobacteria that produce them. The investigation carried out on the biological samples showed that 5.97% of the sample contained carbapenem-resistant microorganisms. This includes 2.20% of urine samples, 0.94% of osteitis samples, 0.63% of wound pus samples, 1.26% of stool samples and 0.94% of blood samples. The microorganisms responsible for this resistance to carbapenems are 5.26% for each of species Arizona, Citrobacter braakii, Enterobacter gergoviae, P. vulgaris, and Serratia ficaria, 26.32% for the species E. gergoviae and P. mirabilis and 21.05% for the species S. odorifera 1. All these enterobacteria produce at least one carbapenemase, which 36.84% are of the KPC type, 10.53% of the OXA-48 or OXA-181 type and 52.63% of types that could not be determined by the algorithm proposed by Nordmann et al. used for this purpose. The types of carbapenemases determined in this revealed 11 substrates and inhibition profiles associated with their production. This highlighted the difficulty of applying an inhibition law in situ in the context of probabilistic antibiotic therapy.