Phenotypic Assessment of Clinical Escherichia coli Isolates as an Indicator for Uropathogenic Potential

Abstract

Urinary tract infections are the second leading infectious disease worldwide, occurring in over half of the female population during their lifetime. Most infections are caused by uropathogenic Escherichia coli (UPEC) strains.

In vitroassays for clinical isolates of sequence type 131Escherichia colido not recapitulatein vivoinfectivity using a murine model of urinary tract infection

“…However, a similar study comparing UTI clinical isolates of several sequence types, found multiple isolates were nonmotile after 16 hours at 30°C with an equal number hypermobile as compared to CFT073; whereas, our study found most clinical isolates more motile than CFT073, with only 3/20 isolates less motile than the lab strain BW25113 or pathogenic CFT073 [14]. The same study found most clinical isolates tested produced less biofilm than CFT073 at 37°C; whereas, in our study we found CFT073 to be comparable to the ST131 clinical isolates yet lower than the lab strains for both static and shaking conditions [14]. The only ST131 isolate tested (HM6) also showed comparable biofilm production to CFT073 in the previous study [14].…”

“…The same study found most clinical isolates tested produced less biofilm than CFT073 at 37°C; whereas, in our study we found CFT073 to be comparable to the ST131 clinical isolates yet lower than the lab strains for both static and shaking conditions [14]. The only ST131 isolate tested (HM6) also showed comparable biofilm production to CFT073 in the previous study [14]. These differences could be due to the divergence of experimental parameters with different temperatures and assay times but are also reflective of the variable nature of clinical isolates in phenotypic testing as noted throughout this paper.…”

Facile Modification of Medical-Grade Silicone for Antimicrobial Effectiveness and Biocompatibility: A Potential Therapeutic Strategy against Bacterial Biofilms

“…When physiological balance is disturbed by disease, surgery, medication, a wound, a foreign body, immunodeficiency, or other circumstance, some commensal organisms coexisting with their human host become opportunistic pathogens. 1 Examples of commensal bacteria that are opportunistic pathogens include Streptococcus pneumoniae (S. pneumoniae), Staphylococcus aureus (S. aureus), uropathogenic Escherichia coli (E. coli, UPEC, motile from the intestine to the urethra), 2 and those from the environment such as Pseudomonas aeruginosa (P. aeruginosa). 3 Opportunistic pathogens readily adhere to hydrophobic silicone and form biofilms.…”

“…aureus), uropathogenic Escherichia coli (E. coli, UPEC, motile from the intestine to the urethra), and those from the environment such as Pseudomonas aeruginosa (P. aeruginosa) .…”

“…coli including motile uropathogenic E. coli (UPEC) usually originating from the intestine. , Endotracheal tubes (ETs) provide life-saving oxygen to the lungs from mechanical ventilation but become coated rapidly with biofilms from host oral and environmental pathogens. Thus, despite the life-saving benefits of intubation, direct access to the lungs often results in Ventilator-Associated Pneumonia (VAP) leading to increased morbidity and high mortality (33–50%) .…”