Phenotypic and transcriptomic responses of two Nilaparvata lugens populations to the Mudgo rice containing Bph1

Wan, Pin-Jun; Zhou, Ruonan; Nanda, Satyabrata; He, Jia-Chun; Yuan, San-Yue; Wang, Weixia; Li, Fengxiang; Fu, Qiang

doi:10.1038/s41598-019-50632-z

Cited by 11 publications

(23 citation statements)

References 57 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a previous study, the result of higher trehalose levels in BPHs feeding on resistant rice than on BPHs feeding on the susceptible varieties has also been documented (Liu et al, 2017). In another study, an TN1‐BPHs population feeding on Mudgo rice containing Bph1 displayed less phloem sap ingestion and decreased trehalose and glucose abundance (Wan et al, 2019). In addition to glucose and trehalose, the glycogen level was also lower in BPHs feeding on ZJZ17, ZZY8, TYHZ, and JLYHZ than in those feeding on TN1 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Brown planthopper infestations alter sugar metabolism in the rice plant as well as brown planthopper

Liu

et al. 2021

Physiol. Entomol.

View full text Add to dashboard Cite

Carbohydrate plays an important role in rice resistance or tolerance to pests, and also affects the continuous feeding of brown planthoppers (BPHs) on rice. However, the change in the sugar metabolism of BPH and rice plant after BPH infestation is not well studied. In this study, the rice variety Taichung Native 1 (TN1) was taken as a control group, and four kinds of common rice varieties in Zhejiang province (China), Zhong Jiazao 17 (ZJZ17), Zhong Zheyou 8 (ZZY8), Tian Youhuazhan (TYHZ), and Jing Liangyouhuazhan (JLYHZ), were taken as experimental group. The results showed that the BPHs best preferred the TN1 rice variety. BPHs in fifth instar feeding on the other four rice varieties had lower levels of trehalose, glucose, and glycogen, and the difference in sugar levels further resulted in differences in trehalose‐6‐phosphate synthase and trehalase expression and trehalase activity. In addition, after BPHs infestation, the glucose content in the sheath at 30 days decreased, while it increased in the sheath at 60 days. Moreover, the trehalose content in the sheath at 30 and 60 days decreased. The starch in the leaves at 60 days also decreased. This study demonstrated that both BPHs and rice plant showed changes in carbohydrate metabolism after BPHs feeding on rice, but these changes varied with rice varieties and rice growth stage. These results provide a basis for a better understanding the physiological interaction between BPH and rice plant.

show abstract

Section: Discussionmentioning

confidence: 99%

Brown planthopper infestations alter sugar metabolism in the rice plant as well as brown planthopper

Liu

et al. 2021

Physiol. Entomol.

View full text Add to dashboard Cite

show abstract

“…Among them, AS has been especially studied in brown planthopper (BPH), Nilaparvata lugens , because this phloem feeder insect pest imposes severe damage to rice—mainly during the reproductive stage [ 152 ]. The ‘hopper burn’ of rice caused by this insect pest often leads to wilting and subsequent plant death, thus devastating the rice yield or even causing the complete yield loss [ 158 ]. Studies suggest that AS may potentially help BPH to feed on rice plants, and possibly contribute to counter diverse defenses against BPH feeding that rice has evolved.…”

Section: Biotic Stressmentioning

confidence: 99%

“…Furthermore, besides their impaired mycelial growth, conidiation, and virulence, the Mogrp1 mutant fungi are unable to cause lesions on rice leaves [ 169 ]. Similarly, the silencing of SR gene NlSRp54 greatly decreases the survival of virulent BPH on resistant Mudgo rice, implicating an essential role of AS in the BPH-rice interaction and possibly in the rice resistance breakdown [ 158 ]. Another splicing factor ‘female determinant factor’ (NlFmd) modulates sex determination in BPH by regulating female-specific AS of the most conserved gene in sex determination cascade doublesex ( Nldsx ), and is, thus, important for female development [ 170 ].…”

Section: Biotic Stressmentioning

confidence: 99%

Stress-Induced Changes in Alternative Splicing Landscape in Rice: Functional Significance of Splice Isoforms in Stress Tolerance

Ganie

Reddy

2021

Biology

View full text Add to dashboard Cite

Improvements in yield and quality of rice are crucial for global food security. However, global rice production is substantially hindered by various biotic and abiotic stresses. Making further improvements in rice yield is a major challenge to the rice research community, which can be accomplished through developing abiotic stress-resilient rice varieties and engineering durable agrochemical-independent pathogen resistance in high-yielding elite rice varieties. This, in turn, needs increased understanding of the mechanisms by which stresses affect rice growth and development. Alternative splicing (AS), a post-transcriptional gene regulatory mechanism, allows rapid changes in the transcriptome and can generate novel regulatory mechanisms to confer plasticity to plant growth and development. Mounting evidence indicates that AS has a prominent role in regulating rice growth and development under stress conditions. Several regulatory and structural genes and splicing factors of rice undergo different types of stress-induced AS events, and the functional significance of some of them in stress tolerance has been defined. Both rice and its pathogens use this complex regulatory mechanism to devise strategies against each other. This review covers the current understanding and evidence for the involvement of AS in biotic and abiotic stress-responsive genes, and its relevance to rice growth and development. Furthermore, we discuss implications of AS for the virulence of different rice pathogens and highlight the areas of further research and potential future avenues to develop climate-smart and disease-resistant rice varieties.

show abstract

“…Qingliu, an indica rice variety, is resistant to C. medinalis [ 24 ]. The indica rice cultivar TN1 is highly susceptible to C. medinalis and N. lugens and was used as a susceptibility check [ 26 , 72 ]. Rice seeds of Qingliu and TN1 were sterilized for 20 mins, rinsed in sterile water and sown on water-moistened filter paper in Petri dishes.…”

Section: Methodsmentioning

confidence: 99%

“…A SSST was used to evaluate the resistance of Qingliu to N. lugens [ 74 ]. Four varieties were used as BPH-resistant and BPH-susceptible checks in the SSST and the following modified seedbox screening test (MSST): Mudgo and H105 were BPH-moderately resistant checks [ 75 ]; TN1 and TNG67 were BPH-susceptible checks [ 72 , 76 ], in addition to Baiqiaowan (a leaffolder-resistant check) [ 24 ]. A total of twenty-four seeds of each rice variety were sown in lines.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomics identifies key defense mechanisms in rice resistant to both leaf-feeding and phloem feeding herbivores

Cheah

Fang

et al. 2021

BMC Plant Biol

View full text Add to dashboard Cite

Background Outbreaks of insect pests in paddy fields cause heavy losses in global rice yield annually, a threat projected to be aggravated by ongoing climate warming. Although significant progress has been made in the screening and cloning of insect resistance genes in rice germplasm and their introgression into modern cultivars, improved rice resistance is only effective against either chewing or phloem-feeding insects. Results In this study, the results from standard and modified seedbox screening, settlement preference and honeydew excretion tests consistently showed that Qingliu, a previously known leaffolder-resistant rice variety, is also moderately resistant to brown planthopper (BPH). High-throughput RNA sequencing showed a higher number of differentially expressed genes (DEGs) at the infestation site, with 2720 DEGs in leaves vs 181 DEGs in sheaths for leaffolder herbivory and 450 DEGs in sheaths vs 212 DEGs in leaves for BPH infestation. The leaf-specific transcriptome revealed that Qingliu responds to leaffolder feeding by activating jasmonic acid biosynthesis genes and genes regulating the shikimate and phenylpropanoid pathways that are essential for the biosynthesis of salicylic acid, melatonin, flavonoids and lignin defensive compounds. The sheath-specific transcriptome revealed that Qingliu responds to BPH infestation by inducing salicylic acid-responsive genes and those controlling cellular signaling cascades. Taken together these genes could play a role in triggering defense mechanisms such as cell wall modifications and cuticular wax formation. Conclusions This study highlighted the key defensive responses of a rarely observed rice variety Qingliu that has resistance to attacks by two different feeding guilds of herbivores. The leaffolders are leaf-feeder while the BPHs are phloem feeders, consequently Qingliu is considered to have dual resistance. Although the defense responses of Qingliu to both insect pest types appear largely dissimilar, the phenylpropanoid pathway (or more specifically phenylalanine ammonia-lyase genes) could be a convergent upstream pathway. However, this possibility requires further studies. This information is valuable for breeding programs aiming to generate broad spectrum insect resistance in rice cultivars.

show abstract

Phenotypic and transcriptomic responses of two Nilaparvata lugens populations to the Mudgo rice containing Bph1

Cited by 11 publications

References 57 publications

Brown planthopper infestations alter sugar metabolism in the rice plant as well as brown planthopper

Brown planthopper infestations alter sugar metabolism in the rice plant as well as brown planthopper

Stress-Induced Changes in Alternative Splicing Landscape in Rice: Functional Significance of Splice Isoforms in Stress Tolerance

Transcriptomics identifies key defense mechanisms in rice resistant to both leaf-feeding and phloem feeding herbivores

Contact Info

Product

Resources

About