Phenotypic and transcriptomic changes in the corneal epithelium following exposure to cigarette smoke

Jin, Mengyi; Wang, Yanzi; An, Xiaoya; Kang, Honghua; Wang, Yixin; Wang, Guoliang; Gao, Yang; Wu, Shi; Reinach, Peter S.; Liu, Zuguo; Xue, Yuhua; Li, Cheng

doi:10.1016/j.envpol.2021.117540

Cited by 6 publications

(4 citation statements)

References 72 publications

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“…This enhanced permeation capacity of the cornea for drug-loaded-LA-grafted-CH-SA nanoparticles compared to CH-SA nano-carriers and drug solution has been perceived in the period of 270 min. The corneal epithelium (outer layer of the cornea) has a naturally hydrophobic nature with the potential of water-repelling [39]. Enhanced corneal penetration of LA-grafted-CH-SA nanoparticles indicates the compatibility of carriers across corneal layers.…”

Section: Discussionmentioning

confidence: 99%

Lauric acid-grafted biopolymeric nanoparticles for efficient Melphalan delivery across the corneal layers for retinoblastoma: ex vivo and in vivo permeation study

Mohseni¹,

Shojaei²,

Naseripour³

et al. 2022

Adv. Nat. Sci: Nanosci. Nanotechnol.

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Intravitreal injection of chemotherapy drugs for intraocular retinoblastoma, for the prevalent pediatric malignancy treatment with the side effects of severe infection. This study tried to synthesize the novel approach to deliver Melphalan to the vitreous cavity through the cornea to increase drug efficacy with non-invasive effects. For this purpose, lauric acid-grafted chitosan-alginate and chitosan-alginate loaded by Melphalan nanoparticles were fabricated. Fourier-transform infrared spectroscopy (FTIR) and x-ray diffraction (XRD) were the basic characterizations of chitosan modification. Scanning electron microscopy (SEM) and dynamic light scattering (DLS) are the other assessments of synthesized nanoparticles. Drug encapsulation and in vitro release were studied using UV-spectroscopy, also ex vivo and in vivo corneal permeation was studied using Franz diffusion cell, fluorescence microscopy, and in vivo animal imaging. FTIR and XRD confirm the successful grafting of lauric acid and spherical shape with the average size of 50 nm and 80 nm observed for modified and chitosan-alginate nanoparticles respectively. In the release study, encapsulation efficiency of 98% with sustained drug release of 80% for 100 h with the Fickian drug diffusion mechanism was reported. Ex vivo corneal permeation exhibited an improved drug penetration rate in the sample of lauric acid-grafted-chitosan-alginate nanoparticles with the results of 5.8 times higher than chitosan-alginate nano-carriers and 15.5-fold compared to drug solution. Also, in vivo study shows that the designed nanoparticles containing Melphalan reached the vitreous cavity with high efficacy. Current investigations illustrate the novel Melphalan delivery with sustained behavior through the cornea with the high potential of corneal layer penetration for retinoblastoma management.

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Section: Discussionmentioning

confidence: 99%

Lauric acid-grafted biopolymeric nanoparticles for efficient Melphalan delivery across the corneal layers for retinoblastoma: ex vivo and in vivo permeation study

Mohseni¹,

Shojaei²,

Naseripour³

et al. 2022

Adv. Nat. Sci: Nanosci. Nanotechnol.

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“…The resulting antioxidant response and the increased mucin expression by conjunctival epithelial cells can be considered an adaptive response to oxidative stress triggered by DEP. Similarly, cigarette smoke extract disrupts the structural integrity of the superficial corneal epithelium damaging the intact epithelial barrier with enhanced oxidative stress response at the same time 108 . The role of oxidative stress has been established in several ocular surface diseases such as dry eyes, uveitis, allergic keratoconjunctivitis 109 .…”

Section: Factors Contributing To Ocular Barrier Dysregulation In Oamentioning

confidence: 99%

“…Similarly, cigarette smoke extract disrupts the structural integrity of the superficial corneal epithelium damaging the intact epithelial barrier with enhanced oxidative stress response at the same time. 108 The role of oxidative stress has been established in several ocular surface diseases such as dry eyes, uveitis, allergic keratoconjunctivitis. 109 Interestingly, higher levels of hydrogen peroxide, a marker of oxidative stress were observed in serum of SAC 110 and in serum and tears of active phase of VKC.…”

Section: Factors Contributing To Ocular Barrier Dysregulation In Oamentioning

confidence: 99%

Epithelial barrier dysfunction in ocular allergy

Singh

Diebold

Sahu

et al. 2021

Allergy

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The epithelial barrier is the first line of defense that forms a protective barrier against pathogens, pollutants, and allergens. Epithelial barrier dysfunction has been recently implicated in the development of allergic diseases such as asthma, atopic dermatitis, food allergy, and rhinitis. However, there is limited knowledge on epithelial barrier dysfunction in ocular allergy (OA). Since the ocular surface is directly exposed to the environment, it is important to understand the role of ocular epithelia and their dysfunction in OA. Impaired epithelial barrier enhances allergen uptake, which lead to activation of immune responses and development of chronic inflammation as seen in allergies. Abnormal expression of tight junction proteins that helps to maintain epithelial integrity has been reported in OA but sufficient data not available in chronic atopic (AKC) and vernal keratoconjunctivitis (VKC), the pathophysiology of which is not just complex, but also the current treatments are not completely effective. This review provides an overview of studies, which indicates the role of barrier dysfunction in OA, and highlights how ocular barrier dysfunction possibly contributes to the disease pathogenesis. The review also explores the potential of ocular epithelial barrier repair strategies as preventive and therapeutic approach.

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“… 3 The corneal epithelium, as the outermost physiological structure of the cornea, is particularly problematic. 4 – 6 Ophthalmologists used corneal fluorescein sodium staining to test the integrity of the corneal epithelium. 7 Different staining shapes, the number of stained dots, and the size of the staining area are often used to distinguish various illnesses or disease severity.…”

Section: Introductionmentioning

confidence: 99%

AES-CSFS: an automatic evaluation system for corneal sodium fluorescein staining based on deep learning

Wang

et al. 2023

Therapeutic Advances in Chronic Disease

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Background: Corneal fluorescein sodium staining is a valuable diagnostic method for various ocular surface diseases. However, the examination results are highly dependent on the subjective experience of ophthalmologists. Objectives: To develop an artificial intelligence system based on deep learning to provide an accurate quantitative assessment of sodium fluorescein staining score and the size of cornea epithelial patchy defect. Design: A prospective study. Methods: We proposed an artificial intelligence system for automatically evaluating corneal staining scores and accurately measuring patchy corneal epithelial defects based on corneal fluorescein sodium staining images. The design incorporates two segmentation models and a classification model to forecast and assess the stained images. Meanwhile, we compare the evaluation findings from the system with ophthalmologists with varying expertise. Results: For the segmentation task of cornea boundary and cornea epithelial patchy defect area, our proposed method can achieve the performance of dice similarity coefficient (DSC) is 0.98/0.97 and Hausdorff distance (HD) is 3.60/8.39, respectively, when compared with the manually labeled gold standard. This method significantly outperforms the four leading algorithms (Unet, Unet++, Swin-Unet, and TransUnet). For the classification task, our algorithm achieves the best performance in accuracy, recall, and F1-score, which are 91.2%, 78.6%, and 79.2%, respectively. The performance of our developed system exceeds seven different approaches (Inception, ShuffleNet, Xception, EfficientNet_B7, DenseNet, ResNet, and VIT) in classification tasks. In addition, three ophthalmologists were selected to rate corneal staining images. The results showed that the performance of our artificial intelligence system significantly outperformed the junior doctors. Conclusion: The system offers a promising automated assessment method for corneal fluorescein staining, decreasing incorrect evaluations caused by ophthalmologists’ subjective variance and limited knowledge.

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Phenotypic and transcriptomic changes in the corneal epithelium following exposure to cigarette smoke

Cited by 6 publications

References 72 publications

Lauric acid-grafted biopolymeric nanoparticles for efficient Melphalan delivery across the corneal layers for retinoblastoma: ex vivo and in vivo permeation study

Lauric acid-grafted biopolymeric nanoparticles for efficient Melphalan delivery across the corneal layers for retinoblastoma: ex vivo and in vivo permeation study

Epithelial barrier dysfunction in ocular allergy

AES-CSFS: an automatic evaluation system for corneal sodium fluorescein staining based on deep learning

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