Phenotypic and transcriptional changes in Escherichia coli K12 in response to simulated microgravity on the EagleStat, a new 2D microgravity analog for bacterial studies.

Topolski, Collin; Divo, Eduardo; Li, Xiaoping; Hicks, Janelle; Chavez, Alba; Castillo, Hugo

doi:10.1016/j.lssr.2022.04.003

Cited by 6 publications

(5 citation statements)

References 43 publications

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“…The rotating cell culture system (RCCS) is a similar type, which uses Petri dish-like vessels. Recently, simpler designs for custom-made devices were validated to make the technology more accessible [ 48 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

Current Knowledge about the Impact of Microgravity on Gene Regulation

Corydon

Schulz

Richter

et al. 2023

Cells

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Microgravity (µg) has a massive impact on the health of space explorers. Microgravity changes the proliferation, differentiation, and growth of cells. As crewed spaceflights into deep space are being planned along with the commercialization of space travelling, researchers have focused on gene regulation in cells and organisms exposed to real (r-) and simulated (s-) µg. In particular, cancer and metastasis research benefits from the findings obtained under µg conditions. Gene regulation is a key factor in a cell or an organism’s ability to sustain life and respond to environmental changes. It is a universal process to control the amount, location, and timing in which genes are expressed. In this review, we provide an overview of µg-induced changes in the numerous mechanisms involved in gene regulation, including regulatory proteins, microRNAs, and the chemical modification of DNA. In particular, we discuss the current knowledge about the impact of microgravity on gene regulation in different types of bacteria, protists, fungi, animals, humans, and cells with a focus on the brain, eye, endothelium, immune system, cartilage, muscle, bone, and various cancers as well as recent findings in plants. Importantly, the obtained data clearly imply that µg experiments can support translational medicine on Earth.

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Section: Resultsmentioning

confidence: 99%

Current Knowledge about the Impact of Microgravity on Gene Regulation

Corydon

Schulz

Richter

et al. 2023

Cells

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“…In previous SMG studies, increased growth was observed in several Escherichia coli strains, Salmonella enterica, Salmonella enteriditis , Stenotrophomonas maltophilia and Vibrio natriegens during growth in LSMMG or spaceflight conditions 27 – 33 , whereas Rhodospirillum rubrum, Staphylococcus aureus and E. coli K12 did not differ in proliferation in LSMMG 15 , 34 , 35 . The aerobic growth rate of Lactobacillus acidophilus was increased in LSMMG but the final cell density did not, while anaerobically grown L. acidophilus displayed no differences in growth rate compared to NG 36 , 37 .…”

Section: Resultsmentioning

confidence: 72%

Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity

Verbeelen,

Fernandez,

Nguyen

et al. 2024

npj Microgravity

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Regenerative life support systems (RLSS) will play a vital role in achieving self-sufficiency during long-distance space travel. Urine conversion into a liquid nitrate-based fertilizer is a key process in most RLSS. This study describes the effects of simulated microgravity (SMG) on Comamonas testosteroni, Nitrosomonas europaea, Nitrobacter winogradskyi and a tripartite culture of the three, in the context of nitrogen recovery for the Micro-Ecological Life Support System Alternative (MELiSSA). Rotary cell culture systems (RCCS) and random positioning machines (RPM) were used as SMG analogues. The transcriptional responses of the cultures were elucidated. For CO2-producing C. testosteroni and the tripartite culture, a PermaLifeTM PL-70 cell culture bag mounted on an in-house 3D-printed holder was applied to eliminate air bubble formation during SMG cultivation. Gene expression changes indicated that the fluid dynamics in SMG caused nutrient and O2 limitation. Genes involved in urea hydrolysis and nitrification were minimally affected, while denitrification-related gene expression was increased. The findings highlight potential challenges for nitrogen recovery in space.

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“…This experiment uses the EagleStat ( 5 ), a 2D clinostat designed for bacterial cultures that runs both treatment (microgravity) and control (gravity) samples simultaneously using regular sterile 2-mL screw-cap plastic tubes as growth vessels ( Fig. 2A and B ).…”

Section: Methodsmentioning

confidence: 99%

“…To explore the phenomenon of microgravity, analogs (such as 2D clinostats) are well-studied systems that simulate microgravity effects on cells and bacteria ( 2 – 4 ) and are an opportunity for hands-on experimentation in undergraduate microbiology laboratories. The objective of this paper is to facilitate the understanding of the biological effects of microgravity on bacteria by measuring the differential development of biofilms on Escherichia coli K12 cultures grown under simulated microgravity conditions for 24 h. For this purpose, we propose a set of experiments that use a 2D clinostat ( 5 ) along with widely used techniques to count bacteria and measure biofilm biomass ( 6 , 7 ). Moreover, this experiment relates undergraduate students in space-related majors to the palpable effects of reduced gravity conditions on microorganisms and supports the development of microbiology skills and scientific analysis by engaging scientific curricula with modern and appealing concepts of space science in traditional microbiology laboratories.…”

Section: Introductionmentioning

confidence: 99%

Use of a microgravity analog to explore the effects of simulated microgravity on the development of Escherichia coli K12 biofilms

Hicks,

Topolski,

Chavez

et al. 2023

J Microbiol Biol Educ.

Self Cite

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The rapid development of space technologies and the increase of human presence in space has brought the discussion of the effects of microgravity on cells into the undergraduate classroom. This paper proposes an idea to simulate microgravity on a bacterial culture, suitable for an introductory microbiology laboratory. For this purpose, we show the use of a 2D clinostat designed for microbial studies, along with traditional microbiology techniques such as optical density, plate counts, and biofilm biomass measurement to test the effect of simulated microgravity on the growth of Escherichia coli K12. This exercise aims to facilitate further discussions on the effects of microgravity on bacteria growth and communication, as well as the use of technology to simulate space and predict physiological changes in cells.

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Phenotypic and transcriptional changes in Escherichia coli K12 in response to simulated microgravity on the EagleStat, a new 2D microgravity analog for bacterial studies.

Cited by 6 publications

References 43 publications

Current Knowledge about the Impact of Microgravity on Gene Regulation

Current Knowledge about the Impact of Microgravity on Gene Regulation

Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity

Use of a microgravity analog to explore the effects of simulated microgravity on the development of Escherichia coli K12 biofilms

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