Phenotypic and proteomic characteristics of sorghum (Sorghum bicolor) albino lethal mutant sbe6-a1

Zhu, Linghua; Wang, Daoping; Sun, Jun; Mu, Yongying; Pu, Weijun; Bo, Ma; Ren, Fuli; Yan, Wenxiu; Zhang, Zhiguo; Li, Guiying; Li, Yubin; Pan, Yinghong

doi:10.1016/j.plaphy.2019.04.001

Cited by 12 publications

(11 citation statements)

References 47 publications

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“…Although not linked to a specific in vivo function during heat stress, a very interesting observation is that the MTI/CP proteins are upregulated as part of the ‘mitochondrial disfunction stimulon’ (Van Aken et al , 2009; De Clercq et al , 2013). This increase in MTI/CP sHSPs also is seen in mutants defective in mitochondrial function, in both Arabidopsis and other plant species (Shedge et al , 2010; Kim et al , 2012; Zhu et al , 2019).…”

Section: Analysis Of Shsp Function During Heat Stress In Vivomentioning

confidence: 85%

Plant small heat shock proteins – evolutionary and functional diversity

2020

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Small heat shock proteins (sHSPs) are an ubiquitous protein family found in archaea, bacteria and eukaryotes. In plants, as in other organisms, sHSPs are upregulated by stress and are proposed to act as molecular chaperones to protect other proteins from stressinduced damage. sHSPs share an 'a-crystallin domain' with a b-sandwich structure and a diverse N-terminal domain. Although sHSPs are 12-25 kDa polypeptides, most assemble into oligomers with ≥ 12 subunits. Plant sHSPs are particularly diverse and numerous; some species have as many as 40 sHSPs. In angiosperms this diversity comprises ≥ 11 sHSP classes encoding proteins targeted to the cytosol, nucleus, endoplasmic reticulum, chloroplasts, mitochondria and peroxisomes. The sHSPs underwent a lineage-specific gene expansion, diversifying early in land plant evolution, potentially in response to stress in the terrestrial environment, and expanded again in seed plants and again in angiosperms. Understanding the structure and evolution of plant sHSPs has progressed, and a model for their chaperone activity has been proposed. However, how the chaperone model applies to diverse sHSPs and what processes sHSPs protect are far from understood. As more plant genomes and transcriptomes become available, it will be possible to explore theories of the evolutionary pressures driving sHSP diversification.

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Section: Analysis Of Shsp Function During Heat Stress In Vivomentioning

confidence: 85%

Plant small heat shock proteins – evolutionary and functional diversity

2020

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“…Proteins were extracted according to Zhu et al [53]. Maize leaves of each sample (100 mg, three biological repeats and three technical repetitions) were used for protein extraction.…”

Section: Protein Extraction and Digestionmentioning

confidence: 99%

“…Polypeptide fragments were separated and analyzed using an Easy nLC 1000 coupled to a Q-Exactive Plus (Thermo Fisher Scientific). Parameter setting of mass spectrometer were the same to Zhu et al [53]. The experiments with each sample were repeated at least three times, and then 54 mass spectrometry raw files with high reliability were obtained (Supplementary data: 54 mass spectrometry raw files, Partner repository with the dataset identifier PXD015819.…”

Section: Mass Spectrometric Analysismentioning

confidence: 99%

Comparative proteomic analysis reveals that the Heterosis of two maize hybrids is related to enhancement of stress response and photosynthesis respectively

Wang

Xiu

et al. 2021

BMC Plant Biol

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Background Heterosis refers to superior traits exhibiting in a hybrid when compared with both parents. Generally, the hybridization between parents can change the expression pattern of some proteins such as non-additive proteins (NAPs) which might lead to heterosis. ‘Zhongdan808’ (ZD808) and ‘Zhongdan909’ (ZD909) are excellent maize hybrids in China, however, the heterosis mechanism of them are not clear. Proteomics has been wildly used in many filed, and comparative proteomic analysis of hybrid and its parents is helpful for understanding the mechanism of heterosis in the two maize hybrids. Results Over 2000 protein groups were quantitatively identified from second seedling leaves of two hybrids and their parents by label-free quantification. Statistical analysis of total identified proteins, differentially accumulated proteins (DAPs) and NAPs of the two hybrids revealed that both of them were more similar to their female parents. In addition, most of DAPs were up-regulated and most of NAPs were high parent abundance or above-high parent abundance in ZD808, while in ZD909, most of DAPs were down-regulated and most of NAPs were low parent abundance or below-low parent abundance. Pathway enrichment analysis showed that more of stress response-related NAPs in ZD808 were high parent abundance or above-high parent abundance, and most of PS related NAPs in ZD909 were high parent abundance or above-high parent abundance. Finally, four stress response-related proteins and eight proteins related to PS were verified by PRM, ten of them had significant differences between hybrid and midparent value. Conclusions Even though every one of the two hybrids were more similar to its female parent at proteome level, the biological basis of heterosis is different in the two maize hybrids. In comparison with their parents, the excellent agronomic traits of hybrid ZD808 is mainly correlated with the high expression levels of some proteins related to stress responses and metabolic functions, while traits of ZD909 is mainly correlated with high expressed proteins related to photosynthesis. Our proteomics results support previous physiological and morphological research and have provided useful information in understanding the reason of valuable agronomic traits.

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“…Total proteins were extracted from the kernels under WW and WS treatments at 10 dap (10 days after treatment) and 25 dap (25 days after treatment) with three biological replicates in accordance, and the methods described by Zhu et al [63] with minor modifications. Approximately 500 mg maize kernels were ground in liquid nitrogen, then dissolved with the lysis buffer and centrifuged at 25,000 g at 4 °C for 15 min.…”

Section: Protein Extractionmentioning

confidence: 99%

Proteomics reveals the effects of drought stress on the kernel development and starch formation of waxy maize

Guo

et al. 2021

BMC Plant Biol

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Background Kernel development and starch formation are the primary determinants of maize yield and quality, which are considerably influenced by drought stress. To clarify the response of maize kernel to drought stress, we established well-watered (WW) and water-stressed (WS) conditions at 1–30 days after pollination (dap) on waxy maize (Zea mays L. sinensis Kulesh). Results Kernel development, starch accumulation, and activities of starch biosynthetic enzymes were significantly reduced by drought stress. The morphology of starch granules changed, whereas the grain filling rate was accelerated. A comparative proteomics approach was applied to analyze the proteome change in kernels under two treatments at 10 dap and 25 dap. Under the WS conditions, 487 and 465 differentially accumulated proteins (DAPs) were identified at 10 dap and 25 dap, respectively. Drought induced the downregulation of proteins involved in the oxidation–reduction process and oxidoreductase, peroxidase, catalase, glutamine synthetase, abscisic acid stress ripening 1, and lipoxygenase, which might be an important reason for the effect of drought stress on kernel development. Notably, several proteins involved in waxy maize endosperm and starch biosynthesis were upregulated at early-kernel stage under WS conditions, which might have accelerated endosperm development and starch synthesis. Additionally, 17 and 11 common DAPs were sustained in the upregulated and downregulated DAP groups, respectively, at 10 dap and 25 dap. Among these 28 proteins, four maize homologs (i.e., A0A1D6H543, B4FTP0, B6SLJ0, and A0A1D6H5J5) were considered as candidate proteins that affected kernel development and drought stress response by comparing with the rice genome. Conclusions The proteomic changes caused by drought were highly correlated with kernel development and starch accumulation, which were closely related to the final yield and quality of waxy maize. Our results provided a foundation for the enhanced understanding of kernel development and starch formation in response to drought stress in waxy maize.

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Phenotypic and proteomic characteristics of sorghum (Sorghum bicolor) albino lethal mutant sbe6-a1

Cited by 12 publications

References 47 publications

Plant small heat shock proteins – evolutionary and functional diversity

Plant small heat shock proteins – evolutionary and functional diversity

Comparative proteomic analysis reveals that the Heterosis of two maize hybrids is related to enhancement of stress response and photosynthesis respectively

Proteomics reveals the effects of drought stress on the kernel development and starch formation of waxy maize

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