Phenotypic and phylogenetic characterization of actinomycetes isolated from Lasius niger and Formica cunicularia ants

Zakalyukina, Yu. V.; Biryukov, Mikhail V.; Golichenkov, M. V.; Нетрусов, А.И.

doi:10.3103/s0096392517010072

Cited by 11 publications

(13 citation statements)

References 13 publications

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“…Therefore, the protective role of identified Actinobacteria in the host-myrmecophile relationship seems likely. Such activity has been described previously in studies on bacteria associated with other ant species, also Formica representatives [65,74,76,[82][83][84][85][86].…”

Section: Discussionsupporting

confidence: 74%

“…The high relative abundance of Actinobacteria and Proteobacteria could be explained through the prism of previous studies. Members of Actinobacteria are able to protect different insect species (e.g., ants and beetles) from pathogens by excretion of substances with antibiotic activity [76]. In our study, Pseudonocardia and Streptomyces have been found (Table S1).…”

Section: Discussionmentioning

confidence: 60%

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First Insight into Microbiome Profiles of Myrmecophilous Beetles and Their Host, Red Wood Ant Formica polyctena (Hymenoptera: Formicidae)—A Case Study

Kaczmarczyk-Ziemba

Zagaja

Wagner

et al. 2020

Insects

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Formica polyctena belongs to the red wood ant species group. Its nests provide a stable, food rich, and temperature and humidity controlled environment, utilized by a wide range of species, called myrmecophiles. Here, we used the high-throughput sequencing of the 16S rRNA gene on the Illumina platform for identification of the microbiome profiles of six selected myrmecophilous beetles (Dendrophilus pygmaeus, Leptacinus formicetorum, Monotoma angusticollis, Myrmechixenus subterraneus, Ptenidium formicetorum and Thiasophila angulata) and their host F. polyctena. Analyzed bacterial communities consisted of a total of 23 phyla, among which Proteobacteria, Actinobacteria, and Firmicutes were the most abundant. Two known endosymbionts—Wolbachia and Rickettsia—were found in the analyzed microbiome profiles and Wolbachia was dominant in bacterial communities associated with F. polyctena, M. subterraneus, L. formicetorum and P. formicetorum (>90% of reads). In turn, M. angusticollis was co-infected with both Wolbachia and Rickettsia, while in the microbiome of T. angulata, the dominance of Rickettsia has been observed. The relationships among the microbiome profiles were complex, and no relative abundance pattern common to all myrmecophilous beetles tested was observed. However, some subtle, species-specific patterns have been observed for bacterial communities associated with D. pygmaeus, M. angusticollis, and T. angulata.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 60%

First Insight into Microbiome Profiles of Myrmecophilous Beetles and Their Host, Red Wood Ant Formica polyctena (Hymenoptera: Formicidae)—A Case Study

Kaczmarczyk-Ziemba

Zagaja

Wagner

et al. 2020

Insects

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“…The extraction of genomic DNA of isolates was achieved using procedures described elsewhere [ 26 ]. For PCR amplification of the 16S rRNA gene, the primers 243F (5′-GGATGAGCCCGCGGCCTA-3′) and A3R (5′-CCAGCCCCACCTTCGAC-3′) were used.…”

Section: Methodsmentioning

confidence: 99%

Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus

et al. 2020

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Antibiotics produced by symbiotic microorganisms were previously shown to be of crucial importance for ecological communities, including ants. Previous works on ant–actinobacteria symbiosis are mainly focused on farming ants, which use antifungal microbial secondary metabolites to control pathogens in their fungal gardens. In this work, we studied microorganisms associated with carpenter ant Camponotus vagus. Pronounced antifungal activity of isolated actinobacteria strain A10 was found to be facilitated by biosynthesis of the antimycin A complex, consisting of small hydrophobic depsipeptides with high antimicrobial and cytotoxic activity. The actinomycete strain A10 was identified as Streptomyces albidoflavus. We studied the antagonistic activity of strain A10 against several entomopathogenic microorganisms. The antifungal activity of this strain potentially indicates a defensive symbiosis with the host ant, producing antimycins to protect carpenter ants against infections. The nature of this ant-microbe association however remains to be established.

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“…The main actinomycetes were representatives of the genus Streptomyces, but Micromonospora and Nocardia were also found. The biodiversity of actinomycetes associated with living ants is considerably lower than in their nests and the surrounding soil [24,25].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Microorganisms Isolated from Ant Nests of Lasius niger

Ефименко

Glukhova

Demiankova

et al. 2020

Life

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In this study, the microbial communities of two nests of black garden ants (Lasius niger) in the hollows of stem branches of old apple trees were found to have similar species compositions: each community contained representatives of three species from the Bacillaceae family and one species of actinomycetes from the genus Streptomyces. In total, four types of bacilli and two actinomycetes were isolated. Actinomycetes were identified as Streptomyces antibioticus-like and Streptomyces sp. None of the bacilli had antibiotic activity, whereas both streptomycetes produced antibiotics that inhibited the growth of Gram-positive bacteria in vitro, including isolates from their community. Antibiotic compounds of S. antibioticus-like strain INA 01148 (Institute of New Antibiotics) were identified as actinomycin D and its closest homologue, actinomycin A. Actinomycins presumably change the microbial community of the ant nest substrate as they act against Gram-positive bacteria and against fungi and Gram-negative bacteria. The antibiotic activity of the isolated Streptomyces sp. INA 01156 is of interest, since the substances produced by this strain inhibit the growth of drug-resistant bacteria, including methicillin-resistant Staphylococcus aureus INA 00761 (MRSA) and vancomycin-resistant strain Leuconostoc mesenteroides VKPM B-4177 (VR) (VKPM–National Collection of Industrial Microorganisms (Russian acronym)).

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Phenotypic and phylogenetic characterization of actinomycetes isolated from Lasius niger and Formica cunicularia ants

Cited by 11 publications

References 13 publications

First Insight into Microbiome Profiles of Myrmecophilous Beetles and Their Host, Red Wood Ant Formica polyctena (Hymenoptera: Formicidae)—A Case Study

First Insight into Microbiome Profiles of Myrmecophilous Beetles and Their Host, Red Wood Ant Formica polyctena (Hymenoptera: Formicidae)—A Case Study

Chemical Ecology of Streptomyces albidoflavus Strain A10 Associated with Carpenter Ant Camponotus vagus

Antimicrobial Activity of Microorganisms Isolated from Ant Nests of Lasius niger

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