Phenotypic and Molecular Study of <i>mecA</i> Gene in MRSA Isolated from Clinical Cases in Misan Province/Iraq

Aziz, Zahid S.; Hassan, Marwa A.

doi:10.5958/0976-5506.2019.00350.4

Cited by 4 publications

(2 citation statements)

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“…This result was in agreement with Madhavan et al 3 who revealed that prevalence of MRSA was 72% and 28% was MSSA. Our results were lower than that was observed by Deniz et al 30 and Aziz & Hassan, 31 who revealed that presence of mecA gene in all S. aureus was 98%, 89.9% respectively.…”

Section: Discussioncontrasting

confidence: 84%

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Awad

ElAziz

adwey

et al. 2022

Egyptian Journal of Medical Microbiology

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Background: Methicillin Resistant Staphylococcus aureus (MRSA) is a serious pathogen in hospitals leading to failure of treatment. MRSA colonization in pediatric patients increases the risk of severe invasive infections, while in the hospital and once they leave. Objectives: We aimed to study the molecular characters of MRSA and its prevalence among pediatric patients and healthcare workers (HCWs) in National Liver Institute (NLI), also the risk factors and mupirocin efficiency in MRSA decolonization were evaluated. Methodology: Nasal and hand swab specimens were obtained from patients and HCWs in pediatric department of NLI. MRSA detection was achieved by phenotypic methods such as cefoxitin diffusion test, VITEK2 compact system and chromogenic media, then confirmed by PCR. Susceptibility of S. aureus to different antibiotics was done by VITEK2 compact system. All isolated MRSA were subjected to SCCmec typing by multiplex PCR. Results: MRSA prevalence among HCWs was 18.9%, and among ward patients, PICU and NICU was 24.2 %, 16.7 % and 36.4 % respectively. Skin infections and less hygiene training were significant risk factors for colonization with MRSA among HCWs. Regarding antibiogram, high resistance rate was detected among MRSA isolates. Sensitivity and specificity of cefoxitin disc diffusion and VITEK2 were 97.8% and 93.3%, while that of CHROM agar was 91.3% and 80%. According to SCCmec typing, the most predominant SCCmec genotypes in the studied subjects was type III followed by type I then type IV, and the least prevalent was type V in both patients and HCWs. So, hospital acquired MRSA (36 /46) was more than community acquired MRSA strains (10/46 samples). When apply intranasal mupirocin ointment 2% regimen, a high successful decolonization rate was achieved. Conclusion: The most predominant type of SCCmec gene was type III followed by type I, which is linked to hospital acquired strains. Implementation of urgent antibiotic policy and strict infection control programs, in addition to mupirocin decolonization measures will decrease the transmission and spread of these multi drug resistant pathogens.

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Section: Discussioncontrasting

confidence: 84%

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Awad

ElAziz

adwey

et al. 2022

Egyptian Journal of Medical Microbiology

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“…Hence, the advanced detection of the highly infective and drug resistance genes in S. aureus is essential in tracking the MDR and hypervirulent strains related to high levels of morbidness/fatality. [7][8][9] Therefore, this study aimed to analyse the frequency of the mecA methicillin resistance gene and to determine antibiotic resistance patterns and phylogeneticity in S. aureus strains isolated from clinical specimens in patients hospitalised at PIMS Islamabad.…”

Section: Introductionmentioning

confidence: 99%

Molecular Diversification and Frequency of mecA Gene in the MRSA Infections of Hospitalized Patients at Islamabad, Pakistan

Rasheed

Yasmine²,

Gul³

et al. 2022

PAFMJ

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Objective: To determine the molecular diversification and frequency of mecA Gene in MRSA infections of hospitalised patients at Islamabad, Pakistan. Study design: Cross-sectional study. Place and Duration: Pakistan Institute of Medical Science, Islamabad, Pakistan, from Sep 2017 to Sep 2018. Methodology: To explore the occurrence of pathogenic S. aureus in the hospitalised patients of Islamabad, 500 samples were collected. All the isolates were further characterised by gram staining, catalase test and DNAse media analysis validating the presence of S.aureus. The overall frequency of S.aureus was 19.5% in all the isolates. Six highly distinguished colonies were further evaluated through colony PCR by 16S r RNA analysis, and results revealed 99.9% homology of isolates with S.aureus. The degree of resistance was further evaluated for 12 isolates based on the previous MRSA resistance pattern. The extraction and concentration of DNA were done by the CTAB and Nanodrop methods. Then primers were designed for the presence of the mecA gene of S. aureus. Results: Results showed 51 (52.7%) MSSA and 45 (47.4%) MRSA among all the isolates. Colony PCR by 16S r RNA analysis results revealed 97 (99.9%) homology of isolates with S.aureus. PCR of all the twelve extracted DNAs of isolates showed the frequency of the mecA gene in all the isolates. Conclusion: The study is the first report the presence of resistance mecA gene in the hospitalised patients of Islamabad. It has depicted a resistant gene's presence and showed the rising trend of resistance in pathogenic S. aureus.

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Drug Resistance Mechanism in Staphylococcus aureus

Musini

Kandula

Giri

2021

Innovations in Biotechnology for a Sustainable Future

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Phenotypic and Molecular Study of mecA Gene in MRSA Isolated from Clinical Cases in Misan Province/Iraq

Cited by 4 publications

References 0 publications

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Molecular characteristics and carriage rate of Methicillin- Resistant Staphylococcus aureus (MRSA) in pediatric hepatology unit

Molecular Diversification and Frequency of mecA Gene in the MRSA Infections of Hospitalized Patients at Islamabad, Pakistan

Drug Resistance Mechanism in Staphylococcus aureus

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