Phenotypic and molecular screening of cassava (Manihot esculentum Crantz) genotypes for resistance to cassava mosaic disease

Asare, Paul A.; Galyuon, Isaac K. A.; Asare‐Bediako, Elvis; Sarfo, Justice Kwabena; Tetteh, J. P.

doi:10.5897/jgmv2014.0056

Cited by 14 publications

(2 citation statements)

References 40 publications

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“…The flanking markers of the three resistance genes to CMD were detected in all highly resistant varieties and the markers linked to CMD1 and CMD2 genes were detected in the resistant varieties. The similar results were reported by previous studies for the flanking markers CMD2 gene [34], [49], [51]. Although CMD3 confers very high levels of resistance to CMD with little or no expression of disease on the leaves [34], some CMD1 and CMD2-type plants become infected with CMGs and develop typical mosaic symptoms [52], [53] but this result depend on CMGs strains used as inoculum.…”

Section: Discussionsupporting

confidence: 89%

“…In addition, the presence of no symptom on the leaves of highly resistant or resistant varieties does not mean that they are not infected with the virus. Indeed, Asare et al (2014) [49], using CMGs strain-specific primers showed through PCR amplification that some varieties displayed no symptom on field were infected with ACMV. This suggests that these varieties are tolerant to ACMV infection whereas those with no PCR amplification band were resistant.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Ten Cassava Varieties for Resistance to Cassava Mosaic Disease in Burkina Faso

Soro¹,

Somé²,

Tiendrébéogo³

et al. 2021

ujar

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The whitefly-transmitted cassava mosaic disease (CMD) caused by cassava mosaic geminiviruses (CMGs) is the most important disease threatening the production of cassava (Manihot esculenta Crantz). Among the main measures for controlling CMD, the use of resistant varieties seems to be among the best methods. This study was conducted in 2017/2018 cassava growing season at three locations to evaluate the level of resistance to CMD of 7 elite cassava varieties widely used by farmers and 3 local cassava varieties cultivated in Burkina Faso. Both morphological and molecular markers were used to screen these varieties against CMGs infection. Morphological markers revealed 8 varieties as highly resistant (TMS 91/02312, TMS 92/0067, TMS 92/0325, TMS 92/0427, TMS 4(2)1425, TMS 94/0270, TMS 30572 and Boborola) whilst the two others (Nouhao and Santidougou) were resistant. The molecular markers linked to CMD1 and CMD2 genes were detected in all varieties. The molecular marker associated with CMD3 gene was detected only in the 8 highly resistant varieties. However, whitefly number per plant and disease pressure were low during this study. It was, therefore, concluded that a better assessment of resistance of cassava varieties to CMD in Burkina Faso could be obtained by combining agro-inoculation and molecular screening.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Evaluation of Ten Cassava Varieties for Resistance to Cassava Mosaic Disease in Burkina Faso

Soro¹,

Somé²,

Tiendrébéogo³

et al. 2021

ujar

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Mutation Breeding in Tropical Root and Tuber Crops

Koundinya¹,

Das²,

Hegde³

2023

Mutation Breeding for Sustainable Food Production and Climate Resilience

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Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria

et al. 2020

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African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) are among the major constraints to cassava productivity within tropical and sub-tropical regions, including Ebonyi State, Nigeria. Thus, virus indexing has become imperative to determine the status of cassava mosaic disease (CMD) in Ebonyi State, to implement appropriate preventive and control strategies. Seventy-eight cassava accessions obtained from different locations of Ebonyi State were phenotypically scored, using scales 1–5 depending on CMD symptomatic expressions, followed by multiplex-PCR and sequencing for validation. 11% of cassava accessions cultivated in Afikpo were resistant (RE) to ACMV compared to 8% of moderately-resistant (MR) accessions in Izzi and 55% of tolerant (TO) ACMV accessions in Ebonyi. 100% of cassava accessions in Onicha and 66% in Afikpo South were susceptible (SU) and highly susceptible (HS) to ACMV, respectively. With multiplex-PCR, 97.4% (ACMV) and 2.6% (EACMV) were positive. Dunn’s multiple comparison tests of CMD mean incidence demonstrated differences (P < 0.05), except between RE and MR, and TO and MR. More transitions (A/G, C/T) compared to transversions (A/T, G/T), were detected, with nonsynonymous mutations (Leucine/Isoleucine; Valine/Isoleucine; Arginine/Lysine; Methionine/Isoleucine), and good bit-scores (91.13–99.07% identites; e-values of 7.00e −148 –0.00e +00 ). Phylogeny resolved the sequences into five major groups. DNA sequencing validated the detected ACMV and EACMV species. This study revealed variants of ACMV and low adoption of RE and MR cassava accessions in the farmers’ fields. The findings will guide in getting disease-free and resistant varieties as planting materials to significantly mitigate the CMD spread in Ebonyi State, Nigeria. Supplementary information The online version of this article (10.1007/s11033-020-06039-5) contains supplementary material, which is available to authorized users.

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Phenotypic and molecular screening of cassava (Manihot esculentum Crantz) genotypes for resistance to cassava mosaic disease

Cited by 14 publications

References 40 publications

Evaluation of Ten Cassava Varieties for Resistance to Cassava Mosaic Disease in Burkina Faso

Evaluation of Ten Cassava Varieties for Resistance to Cassava Mosaic Disease in Burkina Faso

Mutation Breeding in Tropical Root and Tuber Crops

Phenotypic and molecular screenings for determination of cassava mosaic disease (CMD) status in farmers’ fields in Ebonyi State, Nigeria

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