Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China

Yu, Xiaohong; Wan, Zhe; Zhang, Zhenying; Li, Fuqiu; Li, Ruoyu; Liu, Xiaoming

doi:10.1007/s11046-013-9668-6

Cited by 56 publications

(56 citation statements)

References 17 publications

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“…25,26 Our patient was engaged in rural activities that put him at risk of traumatic infection during handling of contaminated materials. 11,25,27 The strain of S. globosa isolated in this study had phenotypic similarities to clinical S. globosa strains reported previously, 11,12,28 although it was able to grow at 37°C, in contrast with the findings of Marimon and others. 5 Although S. globosa is thought to have several clinical manifestations, such as fixed cutaneous, lymphocutaneous, and disseminated cutaneous manifestations, 2,11 clinical reports involving S. globosa mostly have few clinical details.…”

Section: Discussionsupporting

confidence: 49%

“…11,25,27 The strain of S. globosa isolated in this study had phenotypic similarities to clinical S. globosa strains reported previously, 11,12,28 although it was able to grow at 37°C, in contrast with the findings of Marimon and others. 5 Although S. globosa is thought to have several clinical manifestations, such as fixed cutaneous, lymphocutaneous, and disseminated cutaneous manifestations, 2,11 clinical reports involving S. globosa mostly have few clinical details. 9,17 Sporothrix globosa species are considered to be low virulent or even avirulent by some researchers, 13,14 and the large sapronoses occurring in China reflects localized manifestations of the disease.…”

Section: Discussionsupporting

confidence: 49%

“…[5][6][7] The distribution of the species may vary, with S. brasiliensis considered to be geographically restricted, 8,9 and other species, like S. schenckii and S. globosa, being more widespread. [9][10][11][12] The differential virulence, 13,14 transmissibility, 9 and antifungal susceptibility characteristics 15,16 highlight the importance of accurate diagnosis for predicting the clinical outcome.…”

Section: Introductionmentioning

confidence: 99%

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Atypical Clinical Presentation of Sporotrichosis Caused by Sporothrix globosa Resistant to Itraconazole

Gompertz¹,

Rodrigues²,

Fernandes³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Sporotrichosis is a polymorphic disease of humans and animals, which is acquired via traumatic inoculation of Sporothrix propagules into cutaneous or subcutaneous tissue. The etiological agents are in a clinical complex, which includes Sporothrix brasiliensis, Sporothrix schenckii, Sporothrix globosa, and Sporothrix luriei, each of which has specific epidemiological and virulence characteristics. Classical manifestation in humans includes a fixed localized lesion at the site of trauma plus lymphocutaneous sporotrichosis with fungal spreading along the lymphatic channels. Atypical sporotrichosis is a challenge to diagnosis because it can mimic many other dermatological diseases. We report an unusual, itraconazoleresistant cutaneous lesion of sporotrichosis in a 66-year-old Brazilian man. Histopathological examination of the skin revealed vascular and fibroblastic proliferation with chronic granulomatous infiltrate composed of multinucleated giant cells. Sporothrix were isolated from the skin lesion, and phylogenetic analyses confirmed it to be sporotrichosis due to S. globosa, a widespread pathogen. Immunoblotting analysis showed several IgG-reactive molecules in autochthonous preparations of the whole cellular proteins (160, 80, 60, 55, 46, 38,35, and 30 kDa) and exoantigen (35 and 33 kDa). The patient was first unsuccessfully treated with daily itraconazole, and then successfully treated with potassium iodide.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionsupporting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Atypical Clinical Presentation of Sporotrichosis Caused by Sporothrix globosa Resistant to Itraconazole

Gompertz¹,

Rodrigues²,

Fernandes³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…The incidence of sporotrichosis is increasing in China, particularly in the northeastern region of the country (Zhang and Lin, 2008). Sporothrix globosa was identified as the causal agent of sporotrichosis in many of these cases (Tan et al, 2013;Yu et al, 2013;Liu et al, 2014;Zhou et al, 2014). Four species of the Sporothrix complex have been reported as causal agents of sporotrichosis: S. brasiliensis, S. globosa, S. schenckii s.…”

Section: Introductionmentioning

confidence: 99%

Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex

Zhao

Zhou

et al. 2015

J. Zhejiang Univ. Sci. B

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Based on recent molecular data, it has been suggested that Sporothrix globosa is the main causal agent of sporotrichosis in China. The objective of this study was to compare the morphology, growth characteristics, patterns of carbon source usage, and susceptibility to antifungal agents among Sporothrix strains. A total of 15 clinical strains confirmed to be S. globosa, from three different regions of China, and 11 ex-type strains from the CBS-KNAW biodiversity center were obtained. The elongated conidia of S. pallida, S. variecibatus, S. schenckii, and S. schenckii luriei were clearly different from the subglobose and globose conidia of S. globosa strains. S. schenckii is able to assimilate sucrose, raffinose, and ribitol. Susceptibility profiles of these Sporothrix species were evaluated by measuring minimum inhibitory concentrations (MICs). Fluconazole, itraconazole, terbinafine, and amphotericin B showed good activity against most S. globosa clinical isolates from China. Potassium iodide also showed a low MIC against S. pallida, while fluconazole showed a high MIC for S. mexicana, S. humicola, S. globosa, S. schenckii, and S. inflata; these strains might be considered tolerant. The species showed differences in susceptibility to antifungal drugs and should therefore be properly identified during diagnosis prior to designing therapeutic strategies.

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“…Different clinical forms of sporotrichosis are related to the condition of the host, route of infection, and virulence of the pathogen, and virulence may be associated with different Sporothrix species (Arrillaga-Moncrieff et al, 2009;Fernandes et al, 2013). Previous studies have shown that S. globosa is the causal agent of sporotrichosis in China (Tan et al, 2013;Yu et al, 2013;Liu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes

Zhou

2015

J. Zhejiang Univ. Sci. B

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Species included in the Sporothrix schenckii complex are temperature-dependent with dimorphic growth and cause sporotrichosis that is characterized by chronic and fatal lymphocutaneous lesions. The putative species included in the Sporothrix complex are S. brasiliensis, S. globosa, S. mexicana, S. pallida, S. schenckii, and S. lurei. S. globosa is the causal agent of sporotrichosis in China, and its pathogenicity appears to be closely related to the dimorphic transition, i.e. from the mycelial to the yeast phase, it adapts to changing environmental conditions. To determine the molecular mechanisms of the switching process that mediates the dimorphic transition of S. globosa, suppression subtractive hybridization (SSH) was used to prepare a complementary DNA (cDNA) subtraction library from the yeast and mycelial phases. Bioinformatics analysis was performed to profile the relationship between differently expressed genes and the dimorphic transition. Two genes that were expressed at higher levels by the yeast form were selected, and their differential expression levels were verified using a quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR). It is believed that these differently expressed genes are involved in the pathogenesis of S. globosa infection in China.

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Phenotypic and molecular identification of Sporothrix isolates of clinical origin in Northeast China

Cited by 56 publications

References 17 publications

Atypical Clinical Presentation of Sporotrichosis Caused by Sporothrix globosa Resistant to Itraconazole

Atypical Clinical Presentation of Sporotrichosis Caused by Sporothrix globosa Resistant to Itraconazole

Morphological and physiological comparison of taxa comprising the Sporothrix schenckii complex

Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes

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