Phenotypic and molecular diversity among landraces of snapmelon ( Cucumis melo var. momordica) adapted to the hot and humid tropics of eastern India

Dhillon, N.S.; Singh, Jugpreet; Fergany, Mohamed; Monforte, Antonio J.; Sureja, Amish K

doi:10.1017/s1479262109990050

Cited by 35 publications

(17 citation statements)

References 26 publications

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“…The momordica group has been reported to show high levels of genetic diversity [47-49]. In addition, high levels of variability, leading to discrepancies in their taxonomic classification, have been reported for dudaim and flexuosus , as accessions of these groups are sometimes grouped with agrestis types or interspersed with sweet cultivated types of the subspecies melo [9,11,32].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome sequencing for SNP discovery across Cucumis melo

et al. 2012

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BackgroundMelon (Cucumis melo L.) is a highly diverse species that is cultivated worldwide. Recent advances in massively parallel sequencing have begun to allow the study of nucleotide diversity in this species. The Sanger method combined with medium-throughput 454 technology were used in a previous study to analyze the genetic diversity of germplasm representing 3 botanical varieties, yielding a collection of about 40,000 SNPs distributed in 14,000 unigenes. However, the usefulness of this resource is limited as the sequenced genotypes do not represent the whole diversity of the species, which is divided into two subspecies with many botanical varieties variable in plant, flowering, and fruit traits, as well as in stress response. As a first step to extensively document levels and patterns of nucleotide variability across the species, we used the high-throughput SOLiD™ system to resequence the transcriptomes of a set of 67 genotypes that had previously been selected from a core collection representing the extant variation of the entire species.ResultsThe deep transcriptome resequencing of all of the genotypes, grouped into 8 pools (wild African agrestis, Asian agrestis and acidulus, exotic Far Eastern conomon, Indian momordica and Asian dudaim and flexuosus, commercial cantalupensis, subsp. melo Asian and European landraces, Spanish inodorus landraces, and Piel de Sapo breeding lines) yielded about 300 M reads. Short reads were mapped to the recently generated draft genome assembly of the DHL line Piel de Sapo (inodorus) x Songwhan Charmi (conomon) and to a new version of melon transcriptome. Regions with at least 6X coverage were used in SNV calling, generating a melon collection with 303,883 variants. These SNVs were dispersed across the entire C. melo genome, and distributed in 15,064 annotated genes. The number and variability of in silico SNVs differed considerably between pools. Our finding of higher genomic diversity in wild and exotic agrestis melons from India and Africa as compared to commercial cultivars, cultigens and landraces from Eastern Europe, Western Asia and the Mediterranean basin is consistent with the evolutionary history proposed for the species. Group-specific SNVs that will be useful in introgression programs were also detected. In a sample of 143 selected putative SNPs, we verified 93% of the polymorphisms in a panel of 78 genotypes.ConclusionsThis study provides the first comprehensive resequencing data for wild, exotic, and cultivated (landraces and commercial) melon transcriptomes, yielding the largest melon SNP collection available to date and representing a notable sample of the species diversity. This data provides a valuable resource for creating a catalog of allelic variants of melon genes and it will aid in future in-depth studies of population genetics, marker-assisted breeding, and gene identification aimed at developing improved varieties.

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Section: Resultsmentioning

confidence: 99%

Transcriptome sequencing for SNP discovery across Cucumis melo

et al. 2012

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“…trigonus are wild progenitors from which melon was domesticated. We previously reported that melon landraces from North, South and East India exhibit high genetic diversity (Dhillon et al 2007(Dhillon et al , 2009Fergany et al 2011). Further intense and wide collection of wild melon throughout India would, perhaps, help to understand domestication of melon.…”

Section: Discussionmentioning

confidence: 99%

Wild melon diversity in India (Punjab State)

Roy

Bal

Fergany

et al. 2011

Genet Resour Crop Evol

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We present here the first comprehensive genetic characterization of wild melon accessions from northern India. The genetic diversity among 43 wild melon accessions collected from the six agro-ecological regions of the Punjab State of India was assessed by measuring variation at 16 Simple Sequence Repeat (SSR) loci, morphological traits of plant habit and fruit morphological traits, two yield-associated traits, root nematode resistance and biochemical composition (ascorbic acid, carotenoids, titrable acidity). Variation among accessions was observed in plant habit and fruit traits and wild melon germplasm with high acidity and elevated carotenoid content and possessing resistance to Meloidogyne incognita was identified in the collection. A high level of genetic variability in wild melon germplasm was suggested by SSR analysis. Comparative analysis using SSRs of the genetic variability between wild melons from the north and other melons from the south and east regions of India and also reference accessions of cultivated melon from Spain, Japan, Korea, Maldives, Iraq and Israel, showed regional differentiation among Indian melon accessions and that Indian germplasm was not closely related to melon accessions from other parts of the world. A highly drought tolerant accession belonging to var. agrestis Naud. was also identified.

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“…Additionally, 13 Indian accessions (wild agrestis , Momordica, and Acidulus), previously explored by Dhillon et al . (), Fergany et al . () and Roy et al .…”

Section: Methodsmentioning

confidence: 95%

Brazilian melon landraces resistant to Podosphaera xanthii are unique germplasm resources

Nunes

Esteras

Ricarte

et al. 2017

Annals of Applied Biology

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Podosphaera xanthii is the most important causal agent of powdery mildew in melon, a crop ranked within the most economically important species worldwide. The best strategy to face this fungus disease, which causes important production losses, is the development of genetically resistant cultivars. Genetic breeding programmes require sources of resistance, and a few ones have been reported in melon, mostly in Momordica and Acidulus horticultural groups. However, the existence of many races that reduces the durability of the resistance makes necessary to find new resistant genotypes with different genetic backgrounds. In this work, Brazilian germplasm, together with a set of Indian landraces, and the COMAV's (Institute for the Conservation and Breeding of Agricultural Biodiversity) melon core collection, representing the whole variability of the species, were assessed for resistance against some common races in Spain and Brazil and genotyped with a 123-SNP (single nucleotide polymorphisms) genotyping platform to study the molecular relationships of the resistant accessions. In the first experiment, carried out in Valencia (Spain) in 2013, seventy-nine melon accessions were evaluated using artificial inoculation. Five accessions selected as resistant were also evaluated against races 1, 3, and 5 in Mossoró (Brazil, 2015) and against race 3.5 in Valencia (2016) under greenhouse conditions, and under four field conditions in Brazil. The accessions, AL-1, BA-3, CE-3, and RN-2, within the Brazilian collection, presented resistance against all the races of P. xanthii assayed in all conditions tested. AL-1, CE-3 and RN-2 were molecularly more similar to wild agrestis and Acidulus melons from Asia and Africa, while BA-3 grouped with Momordica types. Molecular analysis also confirmed that these new Brazilian sources of resistance differ from those previously reported, constituting interesting materials to encourage genetic breeding programmes, especially in Brazil and Spain.

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Phenotypic and molecular diversity among landraces of snapmelon ( Cucumis melo var. momordica) adapted to the hot and humid tropics of eastern India

Cited by 35 publications

References 26 publications

Transcriptome sequencing for SNP discovery across Cucumis melo

Transcriptome sequencing for SNP discovery across Cucumis melo

Wild melon diversity in India (Punjab State)

Brazilian melon landraces resistant to Podosphaera xanthii are unique germplasm resources

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